Development of SERS substrate using phage-based magnetic template for triplex assay in sepsis diagnosis

Anh H. Nguyen, Yesol Shin, Sang Jun Sim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Development of a new substrate for surface-enhanced Raman scattering (SERS) is one area of interest for the improvement of SERS performance. Herein, we introduce a new method for developing new mesoporous SERS substrates using M13 phages that display cysteine-rich peptides on the pVIII major units, which is an alternative for thiol donor using chemical modifications. Together with the SERS substrate development, and the use of the SERS technique for sepsis diagnostics is a new approach in clinical settings. The substrates were characterized and magnetized with magnetic immuno colloids made of gold-coated magnetic nanoparticles and specific antibodies. Conventionally, the SERS-tags are prepared by using gold nanoparticles and are modified with Raman dyes to immobilize specific antibodies to capture the biomarkers in the serum samples. However, in this method the SERS-tags are bound to the mesoporous substrate via antibody/antigen interactions to form clusters or layer-by-layer assemblies of SERS-tags for Raman signal enhancement. The SERS spectra showed distinct peaks for tags corresponding to three typical sepsis-specific biomarkers for diagnostics with the limit of detection values of 27 pM, 103 pM, and 78 pM for C-reactive protein (CRP), procalcitonin (PCT), and soluble triggering receptor expressed on myeloid cells-1 (sTREM-1), respectively. With such an approach, SERS can be used for clinical purposes and can be improved by phage display modification rather than chemical alternatives.

Original languageEnglish
Pages (from-to)522-528
Number of pages7
JournalBiosensors and Bioelectronics
Volume85
DOIs
Publication statusPublished - 2016 Nov 15

Fingerprint

Bacteriophages
Raman Spectrum Analysis
Raman scattering
Assays
Sepsis
Substrates
Antibodies
Biomarkers
Gold
Nanoparticles
Bacteriophage M13
Gold Colloid
Chemical modification
Calcitonin
Colloids
Myeloid Cells
Antigens
Sulfhydryl Compounds
C-Reactive Protein
Peptides

Keywords

  • Biosensor
  • Mesoporous materials
  • Multiplex detection
  • Plasmonic nanoparticles
  • Sepsis
  • SERS-tags

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Medicine(all)
  • Biomedical Engineering
  • Electrochemistry

Cite this

Development of SERS substrate using phage-based magnetic template for triplex assay in sepsis diagnosis. / Nguyen, Anh H.; Shin, Yesol; Sim, Sang Jun.

In: Biosensors and Bioelectronics, Vol. 85, 15.11.2016, p. 522-528.

Research output: Contribution to journalArticle

@article{c8a0bee199cd467393c4806cb986ca34,
title = "Development of SERS substrate using phage-based magnetic template for triplex assay in sepsis diagnosis",
abstract = "Development of a new substrate for surface-enhanced Raman scattering (SERS) is one area of interest for the improvement of SERS performance. Herein, we introduce a new method for developing new mesoporous SERS substrates using M13 phages that display cysteine-rich peptides on the pVIII major units, which is an alternative for thiol donor using chemical modifications. Together with the SERS substrate development, and the use of the SERS technique for sepsis diagnostics is a new approach in clinical settings. The substrates were characterized and magnetized with magnetic immuno colloids made of gold-coated magnetic nanoparticles and specific antibodies. Conventionally, the SERS-tags are prepared by using gold nanoparticles and are modified with Raman dyes to immobilize specific antibodies to capture the biomarkers in the serum samples. However, in this method the SERS-tags are bound to the mesoporous substrate via antibody/antigen interactions to form clusters or layer-by-layer assemblies of SERS-tags for Raman signal enhancement. The SERS spectra showed distinct peaks for tags corresponding to three typical sepsis-specific biomarkers for diagnostics with the limit of detection values of 27 pM, 103 pM, and 78 pM for C-reactive protein (CRP), procalcitonin (PCT), and soluble triggering receptor expressed on myeloid cells-1 (sTREM-1), respectively. With such an approach, SERS can be used for clinical purposes and can be improved by phage display modification rather than chemical alternatives.",
keywords = "Biosensor, Mesoporous materials, Multiplex detection, Plasmonic nanoparticles, Sepsis, SERS-tags",
author = "Nguyen, {Anh H.} and Yesol Shin and Sim, {Sang Jun}",
year = "2016",
month = "11",
day = "15",
doi = "10.1016/j.bios.2016.05.043",
language = "English",
volume = "85",
pages = "522--528",
journal = "Biosensors",
issn = "0956-5663",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Development of SERS substrate using phage-based magnetic template for triplex assay in sepsis diagnosis

AU - Nguyen, Anh H.

AU - Shin, Yesol

AU - Sim, Sang Jun

PY - 2016/11/15

Y1 - 2016/11/15

N2 - Development of a new substrate for surface-enhanced Raman scattering (SERS) is one area of interest for the improvement of SERS performance. Herein, we introduce a new method for developing new mesoporous SERS substrates using M13 phages that display cysteine-rich peptides on the pVIII major units, which is an alternative for thiol donor using chemical modifications. Together with the SERS substrate development, and the use of the SERS technique for sepsis diagnostics is a new approach in clinical settings. The substrates were characterized and magnetized with magnetic immuno colloids made of gold-coated magnetic nanoparticles and specific antibodies. Conventionally, the SERS-tags are prepared by using gold nanoparticles and are modified with Raman dyes to immobilize specific antibodies to capture the biomarkers in the serum samples. However, in this method the SERS-tags are bound to the mesoporous substrate via antibody/antigen interactions to form clusters or layer-by-layer assemblies of SERS-tags for Raman signal enhancement. The SERS spectra showed distinct peaks for tags corresponding to three typical sepsis-specific biomarkers for diagnostics with the limit of detection values of 27 pM, 103 pM, and 78 pM for C-reactive protein (CRP), procalcitonin (PCT), and soluble triggering receptor expressed on myeloid cells-1 (sTREM-1), respectively. With such an approach, SERS can be used for clinical purposes and can be improved by phage display modification rather than chemical alternatives.

AB - Development of a new substrate for surface-enhanced Raman scattering (SERS) is one area of interest for the improvement of SERS performance. Herein, we introduce a new method for developing new mesoporous SERS substrates using M13 phages that display cysteine-rich peptides on the pVIII major units, which is an alternative for thiol donor using chemical modifications. Together with the SERS substrate development, and the use of the SERS technique for sepsis diagnostics is a new approach in clinical settings. The substrates were characterized and magnetized with magnetic immuno colloids made of gold-coated magnetic nanoparticles and specific antibodies. Conventionally, the SERS-tags are prepared by using gold nanoparticles and are modified with Raman dyes to immobilize specific antibodies to capture the biomarkers in the serum samples. However, in this method the SERS-tags are bound to the mesoporous substrate via antibody/antigen interactions to form clusters or layer-by-layer assemblies of SERS-tags for Raman signal enhancement. The SERS spectra showed distinct peaks for tags corresponding to three typical sepsis-specific biomarkers for diagnostics with the limit of detection values of 27 pM, 103 pM, and 78 pM for C-reactive protein (CRP), procalcitonin (PCT), and soluble triggering receptor expressed on myeloid cells-1 (sTREM-1), respectively. With such an approach, SERS can be used for clinical purposes and can be improved by phage display modification rather than chemical alternatives.

KW - Biosensor

KW - Mesoporous materials

KW - Multiplex detection

KW - Plasmonic nanoparticles

KW - Sepsis

KW - SERS-tags

UR - http://www.scopus.com/inward/record.url?scp=84982189367&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84982189367&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2016.05.043

DO - 10.1016/j.bios.2016.05.043

M3 - Article

C2 - 27209579

AN - SCOPUS:84982189367

VL - 85

SP - 522

EP - 528

JO - Biosensors

JF - Biosensors

SN - 0956-5663

ER -