Development of surface plasmon resonance immunosensor through metal ion affinity and mixed self-assembled monolayer

Sira Lee, Sang Jun Sim, Chulhwan Park, Man Bock Gu, Un Yeon Hwang, Jongheop Yi, Byung Keun Oh, Jinwon Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An immunosensor based on surface plasmon resonance (SPR) with enhanced performance was developed through a mixed self-assembled monolayer. A mixture of 16- mercaptohexadecanic acid (16-MHA) and 1-undecanethiol with various molar ratios was self-assembled on gold (Au) surface and the carboxylic acid groups of 16-MHA were then coordinated to Zn ions by exposing the substrate to an ethanolic solution of Zn(NO3)26H2O. The antibody was immobilized on the SPR surface by exposing the functionalized substrate to the desired solution of antibody in phosphate- buffered saline (PBS) molecules. The film formation in series was confirmed by SPR and atomic force microscopy (AFM). The functionalized surface was applied to develop an SPR immunosensor for detecting human serum albumin (HSA) and the estimated detection limit (DL) was 4.27 nM. The limit value concentration can be well measured between ill and healthy conditions.

Original languageEnglish
Pages (from-to)1695-1700
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume18
Issue number10
Publication statusPublished - 2008 Oct 28

Fingerprint

Surface Plasmon Resonance
Metals
Ions
Immobilized Antibodies
Acids
Atomic Force Microscopy
Carboxylic Acids
Serum Albumin
Gold
Limit of Detection
Phosphates
Antibodies

Keywords

  • Human serum albumin
  • Immunosensor
  • Metal ion
  • Mixed self-assembled monolayer
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Development of surface plasmon resonance immunosensor through metal ion affinity and mixed self-assembled monolayer. / Lee, Sira; Sim, Sang Jun; Park, Chulhwan; Gu, Man Bock; Hwang, Un Yeon; Yi, Jongheop; Oh, Byung Keun; Lee, Jinwon.

In: Journal of Microbiology and Biotechnology, Vol. 18, No. 10, 28.10.2008, p. 1695-1700.

Research output: Contribution to journalArticle

Lee, Sira ; Sim, Sang Jun ; Park, Chulhwan ; Gu, Man Bock ; Hwang, Un Yeon ; Yi, Jongheop ; Oh, Byung Keun ; Lee, Jinwon. / Development of surface plasmon resonance immunosensor through metal ion affinity and mixed self-assembled monolayer. In: Journal of Microbiology and Biotechnology. 2008 ; Vol. 18, No. 10. pp. 1695-1700.
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AU - Oh, Byung Keun

AU - Lee, Jinwon

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