Developmental expression and subcellular distribution of synaptotagmin 11 in rat hippocampus

H. Yeo, H. W. Kim, J. Mo, Dongmin Lee, S. Han, S. Hong, M. J. Koh, Woong Sun, S. Choi, Im Joo Rhyu, Hyun Kim, H. W. Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Synaptotagmins are required for Ca2+-dependent membrane-trafficking in either neuronal synaptic vesicles or cellular membranes. Previous reports suggested that the synaptotagmin 11 (syt11) gene is involved in the development of schizophrenia based on the genomic analysis of patients. Parkin protein binds to the C2 domains of Syt11 which leads to the polyubiquitination of Syt11. However, where and how Syt11 performs its role in the brain is largely unknown. Here, we report that Syt11 is expressed mainly in the brain. In addition, exogenously expressed Syt11 in HEK293 cells can form higher molecular weight complex via its transmembrane domain. Also, Syt11 is targeted to both dendrite and axon compartments. Immunocytochemistry showed that Syt11 is juxtaposed to postsynaptic markers in both excitatory and inhibitory synapses. Both neuroligin 1 and 2, which are postsynaptic cell adhesion molecules and differentially induce excitatory and inhibitory presynapses, respectively, recruit Syt11 in neuron coculture. Immunogold electron microscopy analysis revealed that Syt11 exists mainly in presynaptic neurotransmitter vesicles and plasma membrane, and rarely in postsynaptic sites. These results suggest that Syt11 may contribute to the regulation of neurotransmitter release in the excitatory and inhibitory presynapses, and postsynapse-targeted membrane trafficking in dendrites.

Original languageEnglish
Pages (from-to)35-43
Number of pages9
JournalNeuroscience
Volume225
DOIs
Publication statusPublished - 2012 Dec 6

Fingerprint

Synaptotagmins
Hippocampus
Dendrites
Membranes
Neurotransmitter Agents
Synaptic Vesicles
HEK293 Cells
Brain
Cell Adhesion Molecules
Coculture Techniques
Synapses
Axons
Schizophrenia
Electron Microscopy
Molecular Weight
Immunohistochemistry
Cell Membrane
Neurons
Genes

Keywords

  • Hippocampus
  • Postsynapse
  • Presynapse
  • Synaptic vesicle
  • Synaptotagmin 11

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Developmental expression and subcellular distribution of synaptotagmin 11 in rat hippocampus. / Yeo, H.; Kim, H. W.; Mo, J.; Lee, Dongmin; Han, S.; Hong, S.; Koh, M. J.; Sun, Woong; Choi, S.; Rhyu, Im Joo; Kim, Hyun; Lee, H. W.

In: Neuroscience, Vol. 225, 06.12.2012, p. 35-43.

Research output: Contribution to journalArticle

Yeo, H. ; Kim, H. W. ; Mo, J. ; Lee, Dongmin ; Han, S. ; Hong, S. ; Koh, M. J. ; Sun, Woong ; Choi, S. ; Rhyu, Im Joo ; Kim, Hyun ; Lee, H. W. / Developmental expression and subcellular distribution of synaptotagmin 11 in rat hippocampus. In: Neuroscience. 2012 ; Vol. 225. pp. 35-43.
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