Abstract
Expression of α9 acetylcholine receptor (AChR) mRNA was studied by in situ hybridiza tion in the rat adult and developing cochlea and vestibular inner ear. α9 AChR mRNA was first observed in cochlear hair cells (HCs) at embryonic day 18 (E18), increased markedly after birth, stayed high until postnatal day 10 (P10), and decreased to substantially lower adult levels by P14. High levels of α9 AChR mRNA expression were also noted in the developing nonneuronal structures of the inner sulcus, chondrocytes, and/or osteoblasts in the cochlear capsule and interscalar laminae. Both developing and adult bone marrow cells also expressed intense α9 AChR mRNA. In the vestibular system, α9 AChR mRNA was first observed in HCs at E16 in all sensory epithelia, increased to its highest levels by P0-P4, then decreased slightly to reach adult levels by P10. The results are consistent with the α9 AChR subserving efferent neurotransmission to both cochlear and vestibular HCs. The observation of α9 AChR mRNA in cochlear HCs 2 weeks prior to functional onset in the cochlea further suggests that expression of this gene is not related to HC activity. The observation of substantial nonneuronal expression of α9 AChR mRNA suggests that this receptor also has functions separate from its role in neurotransmission.
| Original language | English |
|---|---|
| Pages (from-to) | 320-331 |
| Number of pages | 12 |
| Journal | Journal of Comparative Neurology |
| Volume | 393 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1998 Apr 13 |
| Externally published | Yes |
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Keywords
- Auditory system
- Neuronal receptor gene
- Rodent
- Vestibular system
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Developmental expression of acetylcholine receptor mRNA in the rat cochlea and vestibular inner ear. / Luo, Lin; Bennett, Thecla; Jung, Hak Hyun; Ryan, Allen F.
In: Journal of Comparative Neurology, Vol. 393, No. 3, 13.04.1998, p. 320-331.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Developmental expression of acetylcholine receptor mRNA in the rat cochlea and vestibular inner ear
AU - Luo, Lin
AU - Bennett, Thecla
AU - Jung, Hak Hyun
AU - Ryan, Allen F.
PY - 1998/4/13
Y1 - 1998/4/13
N2 - Expression of α9 acetylcholine receptor (AChR) mRNA was studied by in situ hybridiza tion in the rat adult and developing cochlea and vestibular inner ear. α9 AChR mRNA was first observed in cochlear hair cells (HCs) at embryonic day 18 (E18), increased markedly after birth, stayed high until postnatal day 10 (P10), and decreased to substantially lower adult levels by P14. High levels of α9 AChR mRNA expression were also noted in the developing nonneuronal structures of the inner sulcus, chondrocytes, and/or osteoblasts in the cochlear capsule and interscalar laminae. Both developing and adult bone marrow cells also expressed intense α9 AChR mRNA. In the vestibular system, α9 AChR mRNA was first observed in HCs at E16 in all sensory epithelia, increased to its highest levels by P0-P4, then decreased slightly to reach adult levels by P10. The results are consistent with the α9 AChR subserving efferent neurotransmission to both cochlear and vestibular HCs. The observation of α9 AChR mRNA in cochlear HCs 2 weeks prior to functional onset in the cochlea further suggests that expression of this gene is not related to HC activity. The observation of substantial nonneuronal expression of α9 AChR mRNA suggests that this receptor also has functions separate from its role in neurotransmission.
AB - Expression of α9 acetylcholine receptor (AChR) mRNA was studied by in situ hybridiza tion in the rat adult and developing cochlea and vestibular inner ear. α9 AChR mRNA was first observed in cochlear hair cells (HCs) at embryonic day 18 (E18), increased markedly after birth, stayed high until postnatal day 10 (P10), and decreased to substantially lower adult levels by P14. High levels of α9 AChR mRNA expression were also noted in the developing nonneuronal structures of the inner sulcus, chondrocytes, and/or osteoblasts in the cochlear capsule and interscalar laminae. Both developing and adult bone marrow cells also expressed intense α9 AChR mRNA. In the vestibular system, α9 AChR mRNA was first observed in HCs at E16 in all sensory epithelia, increased to its highest levels by P0-P4, then decreased slightly to reach adult levels by P10. The results are consistent with the α9 AChR subserving efferent neurotransmission to both cochlear and vestibular HCs. The observation of α9 AChR mRNA in cochlear HCs 2 weeks prior to functional onset in the cochlea further suggests that expression of this gene is not related to HC activity. The observation of substantial nonneuronal expression of α9 AChR mRNA suggests that this receptor also has functions separate from its role in neurotransmission.
KW - Auditory system
KW - Neuronal receptor gene
KW - Rodent
KW - Vestibular system
UR - http://www.scopus.com/inward/record.url?scp=0032513395&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032513395&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1096-9861(19980413)393:3<320::AID-CNE5>3.0.CO;2-3
DO - 10.1002/(SICI)1096-9861(19980413)393:3<320::AID-CNE5>3.0.CO;2-3
M3 - Article
C2 - 9548553
AN - SCOPUS:0032513395
VL - 393
SP - 320
EP - 331
JO - Journal of Comparative Neurology
JF - Journal of Comparative Neurology
SN - 0021-9967
IS - 3
ER -