Developmental expression of acetylcholine receptor mRNA in the rat cochlea and vestibular inner ear

Lin Luo, Thecla Bennett, Hak Hyun Jung, Allen F. Ryan

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Expression of α9 acetylcholine receptor (AChR) mRNA was studied by in situ hybridiza tion in the rat adult and developing cochlea and vestibular inner ear. α9 AChR mRNA was first observed in cochlear hair cells (HCs) at embryonic day 18 (E18), increased markedly after birth, stayed high until postnatal day 10 (P10), and decreased to substantially lower adult levels by P14. High levels of α9 AChR mRNA expression were also noted in the developing nonneuronal structures of the inner sulcus, chondrocytes, and/or osteoblasts in the cochlear capsule and interscalar laminae. Both developing and adult bone marrow cells also expressed intense α9 AChR mRNA. In the vestibular system, α9 AChR mRNA was first observed in HCs at E16 in all sensory epithelia, increased to its highest levels by P0-P4, then decreased slightly to reach adult levels by P10. The results are consistent with the α9 AChR subserving efferent neurotransmission to both cochlear and vestibular HCs. The observation of α9 AChR mRNA in cochlear HCs 2 weeks prior to functional onset in the cochlea further suggests that expression of this gene is not related to HC activity. The observation of substantial nonneuronal expression of α9 AChR mRNA suggests that this receptor also has functions separate from its role in neurotransmission.

Original languageEnglish
Pages (from-to)320-331
Number of pages12
JournalJournal of Comparative Neurology
Volume393
Issue number3
DOIs
Publication statusPublished - 1998 Apr 13
Externally publishedYes

Fingerprint

Cochlea
Cholinergic Receptors
Inner Ear
Messenger RNA
Auditory Hair Cells
Synaptic Transmission
Hair
Vestibular Hair Cells
Observation
Chondrocytes
Osteoblasts
Bone Marrow Cells
Capsules
Epithelium
Parturition
Gene Expression

Keywords

  • Auditory system
  • Neuronal receptor gene
  • Rodent
  • Vestibular system

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Developmental expression of acetylcholine receptor mRNA in the rat cochlea and vestibular inner ear. / Luo, Lin; Bennett, Thecla; Jung, Hak Hyun; Ryan, Allen F.

In: Journal of Comparative Neurology, Vol. 393, No. 3, 13.04.1998, p. 320-331.

Research output: Contribution to journalArticle

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