Developmental toxicity of fipronil in early development of zebrafish (Danio rerio) larvae: Disrupted vascular formation with angiogenic failure and inhibited neurogenesis

Hahyun Park, Jin Young Lee, Sunwoo Park, Gwonhwa Song, Whasun Lim

Research output: Contribution to journalArticle

Abstract

Fipronil has been widely used in agriculture to prevent aggressive insects from damaging agricultural products. Fipronil residues circulate in the environment and they have been detected in non-targeted organisms in aquatic environments. To study the effect of fipronil toxicity on environmental health, 6 h post fertilization (hpf) zebrafish embryos were treated with fipronil for 72 h. LC50 value was obtained by applying varying concentrations of fipronil to zebrafish embryos for 72 h. As zebrafish embryos are useful vertebrate models for studying developmental and genetic findings in toxicology research, they were exposed to fipronil to study detailed elucidating mechanisms with hazardous end points of toxicity. Cell cycle arrest-related apoptosis supported pathological alterations, such as increased mortality, shortened body length, and reduced hatchability. Furthermore, observed heart defects, including edema and irregular heartbeat were caused due to abnormal blood circulation. In transgenic zebrafish models (fli1:eGFP and olig2:dsRED), disrupted blood vessel formations were indicated by eGFP+ endothelial cells. Moreover, neurogenic defects were observed by studying dsRED+ motor neurons and oligodendrocytes. This study demonstrates fipronil accumulation in aquatic environment and its ability to impair essential processes, such as angiogenesis and neurogenesis during early developmental stage of zebrafish, along with general developmental toxicity.

Original languageEnglish
Article number121531
JournalJournal of hazardous materials
Volume385
DOIs
Publication statusPublished - 2020 Mar 5

Fingerprint

Neurogenesis
Zebrafish
Larva
Blood Vessels
Toxicity
toxicity
larva
Agricultural products
Defects
Endothelial cells
embryo
Blood vessels
Hemodynamics
Cell death
Embryonic Structures
Agriculture
Neurons
aquatic environment
defect
Cells

Keywords

  • Cardiac failure
  • Developmental toxicity
  • Embryogenesis
  • Fipronil
  • Neuronal defect

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Developmental toxicity of fipronil in early development of zebrafish (Danio rerio) larvae : Disrupted vascular formation with angiogenic failure and inhibited neurogenesis. / Park, Hahyun; Lee, Jin Young; Park, Sunwoo; Song, Gwonhwa; Lim, Whasun.

In: Journal of hazardous materials, Vol. 385, 121531, 05.03.2020.

Research output: Contribution to journalArticle

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