Abstract
Fipronil has been widely used in agriculture to prevent aggressive insects from damaging agricultural products. Fipronil residues circulate in the environment and they have been detected in non-targeted organisms in aquatic environments. To study the effect of fipronil toxicity on environmental health, 6 h post fertilization (hpf) zebrafish embryos were treated with fipronil for 72 h. LC50 value was obtained by applying varying concentrations of fipronil to zebrafish embryos for 72 h. As zebrafish embryos are useful vertebrate models for studying developmental and genetic findings in toxicology research, they were exposed to fipronil to study detailed elucidating mechanisms with hazardous end points of toxicity. Cell cycle arrest-related apoptosis supported pathological alterations, such as increased mortality, shortened body length, and reduced hatchability. Furthermore, observed heart defects, including edema and irregular heartbeat were caused due to abnormal blood circulation. In transgenic zebrafish models (fli1:eGFP and olig2:dsRED), disrupted blood vessel formations were indicated by eGFP+ endothelial cells. Moreover, neurogenic defects were observed by studying dsRED+ motor neurons and oligodendrocytes. This study demonstrates fipronil accumulation in aquatic environment and its ability to impair essential processes, such as angiogenesis and neurogenesis during early developmental stage of zebrafish, along with general developmental toxicity.
| Original language | English |
|---|---|
| Article number | 121531 |
| Journal | Journal of hazardous materials |
| Volume | 385 |
| DOIs | |
| Publication status | Published - 2020 Mar 5 |
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Keywords
- Cardiac failure
- Developmental toxicity
- Embryogenesis
- Fipronil
- Neuronal defect
ASJC Scopus subject areas
- Environmental Engineering
- Environmental Chemistry
- Waste Management and Disposal
- Pollution
- Health, Toxicology and Mutagenesis
Cite this
Developmental toxicity of fipronil in early development of zebrafish (Danio rerio) larvae : Disrupted vascular formation with angiogenic failure and inhibited neurogenesis. / Park, Hahyun; Lee, Jin Young; Park, Sunwoo; Song, Gwonhwa; Lim, Whasun.
In: Journal of hazardous materials, Vol. 385, 121531, 05.03.2020.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Developmental toxicity of fipronil in early development of zebrafish (Danio rerio) larvae
T2 - Disrupted vascular formation with angiogenic failure and inhibited neurogenesis
AU - Park, Hahyun
AU - Lee, Jin Young
AU - Park, Sunwoo
AU - Song, Gwonhwa
AU - Lim, Whasun
PY - 2020/3/5
Y1 - 2020/3/5
N2 - Fipronil has been widely used in agriculture to prevent aggressive insects from damaging agricultural products. Fipronil residues circulate in the environment and they have been detected in non-targeted organisms in aquatic environments. To study the effect of fipronil toxicity on environmental health, 6 h post fertilization (hpf) zebrafish embryos were treated with fipronil for 72 h. LC50 value was obtained by applying varying concentrations of fipronil to zebrafish embryos for 72 h. As zebrafish embryos are useful vertebrate models for studying developmental and genetic findings in toxicology research, they were exposed to fipronil to study detailed elucidating mechanisms with hazardous end points of toxicity. Cell cycle arrest-related apoptosis supported pathological alterations, such as increased mortality, shortened body length, and reduced hatchability. Furthermore, observed heart defects, including edema and irregular heartbeat were caused due to abnormal blood circulation. In transgenic zebrafish models (fli1:eGFP and olig2:dsRED), disrupted blood vessel formations were indicated by eGFP+ endothelial cells. Moreover, neurogenic defects were observed by studying dsRED+ motor neurons and oligodendrocytes. This study demonstrates fipronil accumulation in aquatic environment and its ability to impair essential processes, such as angiogenesis and neurogenesis during early developmental stage of zebrafish, along with general developmental toxicity.
AB - Fipronil has been widely used in agriculture to prevent aggressive insects from damaging agricultural products. Fipronil residues circulate in the environment and they have been detected in non-targeted organisms in aquatic environments. To study the effect of fipronil toxicity on environmental health, 6 h post fertilization (hpf) zebrafish embryos were treated with fipronil for 72 h. LC50 value was obtained by applying varying concentrations of fipronil to zebrafish embryos for 72 h. As zebrafish embryos are useful vertebrate models for studying developmental and genetic findings in toxicology research, they were exposed to fipronil to study detailed elucidating mechanisms with hazardous end points of toxicity. Cell cycle arrest-related apoptosis supported pathological alterations, such as increased mortality, shortened body length, and reduced hatchability. Furthermore, observed heart defects, including edema and irregular heartbeat were caused due to abnormal blood circulation. In transgenic zebrafish models (fli1:eGFP and olig2:dsRED), disrupted blood vessel formations were indicated by eGFP+ endothelial cells. Moreover, neurogenic defects were observed by studying dsRED+ motor neurons and oligodendrocytes. This study demonstrates fipronil accumulation in aquatic environment and its ability to impair essential processes, such as angiogenesis and neurogenesis during early developmental stage of zebrafish, along with general developmental toxicity.
KW - Cardiac failure
KW - Developmental toxicity
KW - Embryogenesis
KW - Fipronil
KW - Neuronal defect
UR - http://www.scopus.com/inward/record.url?scp=85075400287&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85075400287&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2019.121531
DO - 10.1016/j.jhazmat.2019.121531
M3 - Article
C2 - 31732348
AN - SCOPUS:85075400287
VL - 385
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
SN - 0304-3894
M1 - 121531
ER -