Abstract
A method of detecting Escherichia coli (EC) in in vivo blood with septicemia was sought using a handmade macro three-electrode (ME) system. Towards this end, an analytical ME probe was prepared using mercury immobilized on a carbon nanotube working electrode, and two graphite pencils were used as counter and reference electrodes instead of the expensive Ag/AgCl standard and Pt counter electrode. Under the optimum conditions, the cyclic potential was obtained at a 0.05 V anodic, then the square-wave stripping working range was obtained at 0.5-3.5 ml (3×10<sup>2</sup>∼4×10<sup>2</sup> CFU/1ml) EC. Also under the optimum conditions, the statistic stability was examined in 1 ml EC 15<sup>th</sup> repeat anodic, with a relative standard deviation of RSD=0.98×10<sup>-2</sup>. The developed method was applied to non treated blood, using only one drop. The patient blood with septicemia had a fast accumulation time (30 sec). A diagnostic detection limit of 0.06 ml EC was obtained. The recognition methods can be applied to in vivo vascular blood and real organic fluids, and can be used for poisoning control.
| Original language | English |
|---|---|
| Pages (from-to) | 590-594 |
| Number of pages | 5 |
| Journal | Medicinal Chemistry |
| Volume | 11 |
| Issue number | 6 |
| Publication status | Published - 2015 Jul 1 |
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Keywords
- Diagnosis
- Escherichia coli
- Voltammetry
ASJC Scopus subject areas
- Drug Discovery
Cite this
Diagnostic recognition of Escherichia coli septicemia in in vivo vascular blood. / Choi, DalWoong; Ly, Suw Young.
In: Medicinal Chemistry, Vol. 11, No. 6, 01.07.2015, p. 590-594.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Diagnostic recognition of Escherichia coli septicemia in in vivo vascular blood
AU - Choi, DalWoong
AU - Ly, Suw Young
PY - 2015/7/1
Y1 - 2015/7/1
N2 - A method of detecting Escherichia coli (EC) in in vivo blood with septicemia was sought using a handmade macro three-electrode (ME) system. Towards this end, an analytical ME probe was prepared using mercury immobilized on a carbon nanotube working electrode, and two graphite pencils were used as counter and reference electrodes instead of the expensive Ag/AgCl standard and Pt counter electrode. Under the optimum conditions, the cyclic potential was obtained at a 0.05 V anodic, then the square-wave stripping working range was obtained at 0.5-3.5 ml (3×102∼4×102 CFU/1ml) EC. Also under the optimum conditions, the statistic stability was examined in 1 ml EC 15th repeat anodic, with a relative standard deviation of RSD=0.98×10-2. The developed method was applied to non treated blood, using only one drop. The patient blood with septicemia had a fast accumulation time (30 sec). A diagnostic detection limit of 0.06 ml EC was obtained. The recognition methods can be applied to in vivo vascular blood and real organic fluids, and can be used for poisoning control.
AB - A method of detecting Escherichia coli (EC) in in vivo blood with septicemia was sought using a handmade macro three-electrode (ME) system. Towards this end, an analytical ME probe was prepared using mercury immobilized on a carbon nanotube working electrode, and two graphite pencils were used as counter and reference electrodes instead of the expensive Ag/AgCl standard and Pt counter electrode. Under the optimum conditions, the cyclic potential was obtained at a 0.05 V anodic, then the square-wave stripping working range was obtained at 0.5-3.5 ml (3×102∼4×102 CFU/1ml) EC. Also under the optimum conditions, the statistic stability was examined in 1 ml EC 15th repeat anodic, with a relative standard deviation of RSD=0.98×10-2. The developed method was applied to non treated blood, using only one drop. The patient blood with septicemia had a fast accumulation time (30 sec). A diagnostic detection limit of 0.06 ml EC was obtained. The recognition methods can be applied to in vivo vascular blood and real organic fluids, and can be used for poisoning control.
KW - Diagnosis
KW - Escherichia coli
KW - Voltammetry
UR - http://www.scopus.com/inward/record.url?scp=84938782502&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84938782502&partnerID=8YFLogxK
M3 - Article
C2 - 25544286
AN - SCOPUS:84938782502
VL - 11
SP - 590
EP - 594
JO - Medicinal Chemistry
JF - Medicinal Chemistry
SN - 1573-4064
IS - 6
ER -