Diagnostic utility of anti-Saccharomyces cerevisiae antibody (ASCA) and Interferon-? assay in the differential diagnosis of Crohn's disease and intestinal tuberculosis

You Sun Kim, Young Ho Kim, Won Ho Kim, Joo Sung Kim, Young Sook Park, Suk Kyun Yang, Byong Duk Ye, Byung Ik Jang, Sung Ae Jung, Yoon Tae Jeen, Jae Hee Cheon, Yong Sung Choi, Jai Hyun Choi, Beom Jin Kim, Chang Hwan Choi, Dong Soo Han

Research output: Contribution to journalArticle

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Abstract

Background: Differential diagnosis of Crohn's disease (CD) from intestinal tuberculosis (ITB) is challenging. Anti-Saccharomyces cerevisiae antibody (ASCA) is a specific serological marker for CD and INF-gamma assay (QuantiFERON-TB gold test, QFT) is a good supplementary diagnostic tool for ITB. We evaluated the clinical usefulness of ASCA and QFT for differential diagnosis of CD from ITB in Korean adults. Methods: A total of 147 patients suspected to have ITB or CD were prospectively enrolled from 13 hospitals. ASCA IgG and IgA serum titers were measured by ELISA, and the QFT test was also performed. Results: Thirty-two of 72 (44.4%) patients with CD were ASCA positive (titer > 25. U) compared to 10 of 75 ITB patients (13.3%) and 3 of 20 healthy controls (15%) (p< 0.01). The QFT test was positive in 7 patients with CD (9.7%) and 50 patients with ITB (66.6%) (p< 0.01). In cases which ASCA positive/QFT negative, the sensitivity, specificity, positive predictive value, and negative predictive value for the diagnosis of CD were 44.4%, 96.0%, 91.4%, and 64.3%, respectively. Conclusion: ASCA is a useful diagnostic tool for CD in Korea, where ITB is prevalent. In particular, when ASCA is combined with QFT, effective differential diagnosis of CD from ITB is possible.

Original languageEnglish
Pages (from-to)1527-1532
Number of pages6
JournalClinica Chimica Acta
Volume412
Issue number17-18
DOIs
Publication statusPublished - 2011 Aug 17

Fingerprint

Crohn Disease
Yeast
Interferons
Saccharomyces cerevisiae
Assays
Tuberculosis
Differential Diagnosis
Gold
Antibodies
Intestinal Diseases
Korea
Immunoglobulin A
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Sensitivity and Specificity
Serum

Keywords

  • Anti-Saccharomyces cerevisiae antibody
  • Crohn's disease
  • INF-gamma assay
  • Intestinal tuberculosis

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Diagnostic utility of anti-Saccharomyces cerevisiae antibody (ASCA) and Interferon-? assay in the differential diagnosis of Crohn's disease and intestinal tuberculosis. / Kim, You Sun; Kim, Young Ho; Kim, Won Ho; Kim, Joo Sung; Park, Young Sook; Yang, Suk Kyun; Ye, Byong Duk; Jang, Byung Ik; Jung, Sung Ae; Jeen, Yoon Tae; Cheon, Jae Hee; Choi, Yong Sung; Choi, Jai Hyun; Kim, Beom Jin; Choi, Chang Hwan; Han, Dong Soo.

In: Clinica Chimica Acta, Vol. 412, No. 17-18, 17.08.2011, p. 1527-1532.

Research output: Contribution to journalArticle

Kim, YS, Kim, YH, Kim, WH, Kim, JS, Park, YS, Yang, SK, Ye, BD, Jang, BI, Jung, SA, Jeen, YT, Cheon, JH, Choi, YS, Choi, JH, Kim, BJ, Choi, CH & Han, DS 2011, 'Diagnostic utility of anti-Saccharomyces cerevisiae antibody (ASCA) and Interferon-? assay in the differential diagnosis of Crohn's disease and intestinal tuberculosis', Clinica Chimica Acta, vol. 412, no. 17-18, pp. 1527-1532. https://doi.org/10.1016/j.cca.2011.04.029
Kim, You Sun ; Kim, Young Ho ; Kim, Won Ho ; Kim, Joo Sung ; Park, Young Sook ; Yang, Suk Kyun ; Ye, Byong Duk ; Jang, Byung Ik ; Jung, Sung Ae ; Jeen, Yoon Tae ; Cheon, Jae Hee ; Choi, Yong Sung ; Choi, Jai Hyun ; Kim, Beom Jin ; Choi, Chang Hwan ; Han, Dong Soo. / Diagnostic utility of anti-Saccharomyces cerevisiae antibody (ASCA) and Interferon-? assay in the differential diagnosis of Crohn's disease and intestinal tuberculosis. In: Clinica Chimica Acta. 2011 ; Vol. 412, No. 17-18. pp. 1527-1532.
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abstract = "Background: Differential diagnosis of Crohn's disease (CD) from intestinal tuberculosis (ITB) is challenging. Anti-Saccharomyces cerevisiae antibody (ASCA) is a specific serological marker for CD and INF-gamma assay (QuantiFERON-TB gold test, QFT) is a good supplementary diagnostic tool for ITB. We evaluated the clinical usefulness of ASCA and QFT for differential diagnosis of CD from ITB in Korean adults. Methods: A total of 147 patients suspected to have ITB or CD were prospectively enrolled from 13 hospitals. ASCA IgG and IgA serum titers were measured by ELISA, and the QFT test was also performed. Results: Thirty-two of 72 (44.4{\%}) patients with CD were ASCA positive (titer > 25. U) compared to 10 of 75 ITB patients (13.3{\%}) and 3 of 20 healthy controls (15{\%}) (p< 0.01). The QFT test was positive in 7 patients with CD (9.7{\%}) and 50 patients with ITB (66.6{\%}) (p< 0.01). In cases which ASCA positive/QFT negative, the sensitivity, specificity, positive predictive value, and negative predictive value for the diagnosis of CD were 44.4{\%}, 96.0{\%}, 91.4{\%}, and 64.3{\%}, respectively. Conclusion: ASCA is a useful diagnostic tool for CD in Korea, where ITB is prevalent. In particular, when ASCA is combined with QFT, effective differential diagnosis of CD from ITB is possible.",
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T1 - Diagnostic utility of anti-Saccharomyces cerevisiae antibody (ASCA) and Interferon-? assay in the differential diagnosis of Crohn's disease and intestinal tuberculosis

AU - Kim, You Sun

AU - Kim, Young Ho

AU - Kim, Won Ho

AU - Kim, Joo Sung

AU - Park, Young Sook

AU - Yang, Suk Kyun

AU - Ye, Byong Duk

AU - Jang, Byung Ik

AU - Jung, Sung Ae

AU - Jeen, Yoon Tae

AU - Cheon, Jae Hee

AU - Choi, Yong Sung

AU - Choi, Jai Hyun

AU - Kim, Beom Jin

AU - Choi, Chang Hwan

AU - Han, Dong Soo

PY - 2011/8/17

Y1 - 2011/8/17

N2 - Background: Differential diagnosis of Crohn's disease (CD) from intestinal tuberculosis (ITB) is challenging. Anti-Saccharomyces cerevisiae antibody (ASCA) is a specific serological marker for CD and INF-gamma assay (QuantiFERON-TB gold test, QFT) is a good supplementary diagnostic tool for ITB. We evaluated the clinical usefulness of ASCA and QFT for differential diagnosis of CD from ITB in Korean adults. Methods: A total of 147 patients suspected to have ITB or CD were prospectively enrolled from 13 hospitals. ASCA IgG and IgA serum titers were measured by ELISA, and the QFT test was also performed. Results: Thirty-two of 72 (44.4%) patients with CD were ASCA positive (titer > 25. U) compared to 10 of 75 ITB patients (13.3%) and 3 of 20 healthy controls (15%) (p< 0.01). The QFT test was positive in 7 patients with CD (9.7%) and 50 patients with ITB (66.6%) (p< 0.01). In cases which ASCA positive/QFT negative, the sensitivity, specificity, positive predictive value, and negative predictive value for the diagnosis of CD were 44.4%, 96.0%, 91.4%, and 64.3%, respectively. Conclusion: ASCA is a useful diagnostic tool for CD in Korea, where ITB is prevalent. In particular, when ASCA is combined with QFT, effective differential diagnosis of CD from ITB is possible.

AB - Background: Differential diagnosis of Crohn's disease (CD) from intestinal tuberculosis (ITB) is challenging. Anti-Saccharomyces cerevisiae antibody (ASCA) is a specific serological marker for CD and INF-gamma assay (QuantiFERON-TB gold test, QFT) is a good supplementary diagnostic tool for ITB. We evaluated the clinical usefulness of ASCA and QFT for differential diagnosis of CD from ITB in Korean adults. Methods: A total of 147 patients suspected to have ITB or CD were prospectively enrolled from 13 hospitals. ASCA IgG and IgA serum titers were measured by ELISA, and the QFT test was also performed. Results: Thirty-two of 72 (44.4%) patients with CD were ASCA positive (titer > 25. U) compared to 10 of 75 ITB patients (13.3%) and 3 of 20 healthy controls (15%) (p< 0.01). The QFT test was positive in 7 patients with CD (9.7%) and 50 patients with ITB (66.6%) (p< 0.01). In cases which ASCA positive/QFT negative, the sensitivity, specificity, positive predictive value, and negative predictive value for the diagnosis of CD were 44.4%, 96.0%, 91.4%, and 64.3%, respectively. Conclusion: ASCA is a useful diagnostic tool for CD in Korea, where ITB is prevalent. In particular, when ASCA is combined with QFT, effective differential diagnosis of CD from ITB is possible.

KW - Anti-Saccharomyces cerevisiae antibody

KW - Crohn's disease

KW - INF-gamma assay

KW - Intestinal tuberculosis

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