Dibenzoylmethane ameliorates lipid-induced inflammation and oxidative injury in diabetic nephropathy

Eun Soo Lee, Mi Hye Kwon, Hong Min Kim, Nami Kim, You Mi Kim, Hyeon Soo Kim, Eun Young Lee, Choon Hee Chung

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Dibenzoylmethane (DBM) is a beta-diketone analog of curcumin. Numerous studies have shown the beneficial effects of curcumin on diabetes, obesity and diabetic complications including diabetic nephropathy. Recently, we investigated the beneficial metabolic effects of DBM on high-fat diet-induced obesity. However, the effects and mechanisms of action of DBM in the kidney are currently unknown. To investigate the renoprotective effects of DBM in type 2 diabetes, we administered DBM (100 mg/kg) orally for 12 weeks to high-fat diet-induced diabetic model mice. We used mouse renal mesangial (MES13) and macrophage (RAW 264.7) cells to examine the mechanism of action of DBM (20 μM). After DBM treatment, the albumin-to-creatinine ratio was significantly decreased compared to that of the high-fat-diet group. Moreover, damaged renal ultra-structures and functions including increased glomerular volume, glomerular basement membrane thickness and inflammatory signals were ameliorated after DBM treatment. Stimulation of MES13 and RAW264.7 cells by palmitate or high-dose glucose with lipopolysaccharides increased inflammatory signals and macrophage migration. However, these changes were reversed by DBM treatment. In addition, DBM inhibited NADPH oxidase 2 and 4 expression and oxidative DNA damage. Collectively, these data suggested that DBM prevented diabetes-induced renal injury through its anti-inflammatory and antioxidant effects.

Original languageEnglish
Pages (from-to)169-179
Number of pages11
JournalThe Journal of endocrinology
Volume240
Issue number2
DOIs
Publication statusPublished - 2019 Feb 1

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Diabetic Nephropathies
Inflammation
Lipids
Wounds and Injuries
High Fat Diet
Kidney
Curcumin
Diabetes Complications
dibenzoylmethane
Obesity
Macrophages
Glomerular Basement Membrane
NADPH Oxidase
Palmitates
Type 2 Diabetes Mellitus
DNA Damage
Lipopolysaccharides
Albumins
Creatinine
Anti-Inflammatory Agents

Keywords

  • diabetic nephropathy
  • dibenzoylmethane
  • inflammation
  • lipotoxicity
  • oxidative stress

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Dibenzoylmethane ameliorates lipid-induced inflammation and oxidative injury in diabetic nephropathy. / Lee, Eun Soo; Kwon, Mi Hye; Kim, Hong Min; Kim, Nami; Kim, You Mi; Kim, Hyeon Soo; Lee, Eun Young; Chung, Choon Hee.

In: The Journal of endocrinology, Vol. 240, No. 2, 01.02.2019, p. 169-179.

Research output: Contribution to journalArticle

Lee, Eun Soo ; Kwon, Mi Hye ; Kim, Hong Min ; Kim, Nami ; Kim, You Mi ; Kim, Hyeon Soo ; Lee, Eun Young ; Chung, Choon Hee. / Dibenzoylmethane ameliorates lipid-induced inflammation and oxidative injury in diabetic nephropathy. In: The Journal of endocrinology. 2019 ; Vol. 240, No. 2. pp. 169-179.
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