Dietary hempseed meal intake increases body growth and shortens the larval stage via the upregulation of cell growth and sterol levels in Drosophila melanogaster

Min Jung Lee, Min Soo Park, Soojin Hwang, Yoon Ki Hong, Gahee Choi, Yoon Seak Suh, Seung Yeop Han, Darae Kim, Jungae Jeun, Chun Taek Oh, Sung-Joon Lee, Sung Jun Han, Donghak Kim, Eun Soo Kim, Gilsang Jeong, Kyoung Sang Cho

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Hempseed, a rich source of polyunsaturated fatty acids (PUFAs) and phytosterols, has been recognized as a potential therapeutic food used for cardioprotection, preventing platelet aggregation, and improving atopic dermatitis. Although several studies have revealed the physiological benefits of hempseed on a variety of animals, the effects of dietary hempseed intake on animal development are currently unknown. In this study, we evaluated the developmental effects of the addition of hempseed meal (HSM) to the diet of Drosophila. Interestingly, dietary HSM intake was shown to increase the body size of flies by increasing cell numbers, and also truncated the larval period without affecting survival rate or longevity. The oviposition of female flies was also increased by dietary HSM supplementation. Interestingly, the levels of sterols, which are precursors of ecdysone, a molting hormone, were found to be elevated in the larvae fed on HSM. Additionally, the hexane extracts of hempseed mimicked the effects of HSM on growth, developmental timing, and reproduction. Moreover, among the major nonpolar components of HSM, feeding on cholesterol but not PUFA mix or campesterol accelerated pupariation and increased body size. These results indicate that the dietary intake of HSM accelerates both body growth and developmental rates in Drosophila via the stimulation of cell growth and ecdysone synthesis. Additionally, nonpolar components of hempseed, such as cholesterol, might be responsible for the effects of HSM on development and reproduction.

Original languageEnglish
Pages (from-to)29-36
Number of pages8
JournalMolecules and Cells
Volume30
Issue number1
DOIs
Publication statusPublished - 2010 Jul 1

Fingerprint

Sterols
Drosophila melanogaster
Meals
Up-Regulation
Growth
Ecdysone
Body Size
Unsaturated Fatty Acids
Diptera
Drosophila
Reproduction
Cholesterol
Phytosterols
Oviposition
Hexanes
Atopic Dermatitis
Platelet Aggregation
Larva
Cell Count
Diet

Keywords

  • body growth
  • cholesterol
  • developmental rate
  • Drosophila
  • ecdysone
  • hempseed meal
  • reproduction

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Dietary hempseed meal intake increases body growth and shortens the larval stage via the upregulation of cell growth and sterol levels in Drosophila melanogaster. / Lee, Min Jung; Park, Min Soo; Hwang, Soojin; Hong, Yoon Ki; Choi, Gahee; Suh, Yoon Seak; Han, Seung Yeop; Kim, Darae; Jeun, Jungae; Oh, Chun Taek; Lee, Sung-Joon; Han, Sung Jun; Kim, Donghak; Kim, Eun Soo; Jeong, Gilsang; Cho, Kyoung Sang.

In: Molecules and Cells, Vol. 30, No. 1, 01.07.2010, p. 29-36.

Research output: Contribution to journalArticle

Lee, MJ, Park, MS, Hwang, S, Hong, YK, Choi, G, Suh, YS, Han, SY, Kim, D, Jeun, J, Oh, CT, Lee, S-J, Han, SJ, Kim, D, Kim, ES, Jeong, G & Cho, KS 2010, 'Dietary hempseed meal intake increases body growth and shortens the larval stage via the upregulation of cell growth and sterol levels in Drosophila melanogaster', Molecules and Cells, vol. 30, no. 1, pp. 29-36. https://doi.org/10.1007/s10059-010-0085-0
Lee, Min Jung ; Park, Min Soo ; Hwang, Soojin ; Hong, Yoon Ki ; Choi, Gahee ; Suh, Yoon Seak ; Han, Seung Yeop ; Kim, Darae ; Jeun, Jungae ; Oh, Chun Taek ; Lee, Sung-Joon ; Han, Sung Jun ; Kim, Donghak ; Kim, Eun Soo ; Jeong, Gilsang ; Cho, Kyoung Sang. / Dietary hempseed meal intake increases body growth and shortens the larval stage via the upregulation of cell growth and sterol levels in Drosophila melanogaster. In: Molecules and Cells. 2010 ; Vol. 30, No. 1. pp. 29-36.
@article{da8f1d1dab184eceb03a5e954116ab5f,
title = "Dietary hempseed meal intake increases body growth and shortens the larval stage via the upregulation of cell growth and sterol levels in Drosophila melanogaster",
abstract = "Hempseed, a rich source of polyunsaturated fatty acids (PUFAs) and phytosterols, has been recognized as a potential therapeutic food used for cardioprotection, preventing platelet aggregation, and improving atopic dermatitis. Although several studies have revealed the physiological benefits of hempseed on a variety of animals, the effects of dietary hempseed intake on animal development are currently unknown. In this study, we evaluated the developmental effects of the addition of hempseed meal (HSM) to the diet of Drosophila. Interestingly, dietary HSM intake was shown to increase the body size of flies by increasing cell numbers, and also truncated the larval period without affecting survival rate or longevity. The oviposition of female flies was also increased by dietary HSM supplementation. Interestingly, the levels of sterols, which are precursors of ecdysone, a molting hormone, were found to be elevated in the larvae fed on HSM. Additionally, the hexane extracts of hempseed mimicked the effects of HSM on growth, developmental timing, and reproduction. Moreover, among the major nonpolar components of HSM, feeding on cholesterol but not PUFA mix or campesterol accelerated pupariation and increased body size. These results indicate that the dietary intake of HSM accelerates both body growth and developmental rates in Drosophila via the stimulation of cell growth and ecdysone synthesis. Additionally, nonpolar components of hempseed, such as cholesterol, might be responsible for the effects of HSM on development and reproduction.",
keywords = "body growth, cholesterol, developmental rate, Drosophila, ecdysone, hempseed meal, reproduction",
author = "Lee, {Min Jung} and Park, {Min Soo} and Soojin Hwang and Hong, {Yoon Ki} and Gahee Choi and Suh, {Yoon Seak} and Han, {Seung Yeop} and Darae Kim and Jungae Jeun and Oh, {Chun Taek} and Sung-Joon Lee and Han, {Sung Jun} and Donghak Kim and Kim, {Eun Soo} and Gilsang Jeong and Cho, {Kyoung Sang}",
year = "2010",
month = "7",
day = "1",
doi = "10.1007/s10059-010-0085-0",
language = "English",
volume = "30",
pages = "29--36",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "1",

}

TY - JOUR

T1 - Dietary hempseed meal intake increases body growth and shortens the larval stage via the upregulation of cell growth and sterol levels in Drosophila melanogaster

AU - Lee, Min Jung

AU - Park, Min Soo

AU - Hwang, Soojin

AU - Hong, Yoon Ki

AU - Choi, Gahee

AU - Suh, Yoon Seak

AU - Han, Seung Yeop

AU - Kim, Darae

AU - Jeun, Jungae

AU - Oh, Chun Taek

AU - Lee, Sung-Joon

AU - Han, Sung Jun

AU - Kim, Donghak

AU - Kim, Eun Soo

AU - Jeong, Gilsang

AU - Cho, Kyoung Sang

PY - 2010/7/1

Y1 - 2010/7/1

N2 - Hempseed, a rich source of polyunsaturated fatty acids (PUFAs) and phytosterols, has been recognized as a potential therapeutic food used for cardioprotection, preventing platelet aggregation, and improving atopic dermatitis. Although several studies have revealed the physiological benefits of hempseed on a variety of animals, the effects of dietary hempseed intake on animal development are currently unknown. In this study, we evaluated the developmental effects of the addition of hempseed meal (HSM) to the diet of Drosophila. Interestingly, dietary HSM intake was shown to increase the body size of flies by increasing cell numbers, and also truncated the larval period without affecting survival rate or longevity. The oviposition of female flies was also increased by dietary HSM supplementation. Interestingly, the levels of sterols, which are precursors of ecdysone, a molting hormone, were found to be elevated in the larvae fed on HSM. Additionally, the hexane extracts of hempseed mimicked the effects of HSM on growth, developmental timing, and reproduction. Moreover, among the major nonpolar components of HSM, feeding on cholesterol but not PUFA mix or campesterol accelerated pupariation and increased body size. These results indicate that the dietary intake of HSM accelerates both body growth and developmental rates in Drosophila via the stimulation of cell growth and ecdysone synthesis. Additionally, nonpolar components of hempseed, such as cholesterol, might be responsible for the effects of HSM on development and reproduction.

AB - Hempseed, a rich source of polyunsaturated fatty acids (PUFAs) and phytosterols, has been recognized as a potential therapeutic food used for cardioprotection, preventing platelet aggregation, and improving atopic dermatitis. Although several studies have revealed the physiological benefits of hempseed on a variety of animals, the effects of dietary hempseed intake on animal development are currently unknown. In this study, we evaluated the developmental effects of the addition of hempseed meal (HSM) to the diet of Drosophila. Interestingly, dietary HSM intake was shown to increase the body size of flies by increasing cell numbers, and also truncated the larval period without affecting survival rate or longevity. The oviposition of female flies was also increased by dietary HSM supplementation. Interestingly, the levels of sterols, which are precursors of ecdysone, a molting hormone, were found to be elevated in the larvae fed on HSM. Additionally, the hexane extracts of hempseed mimicked the effects of HSM on growth, developmental timing, and reproduction. Moreover, among the major nonpolar components of HSM, feeding on cholesterol but not PUFA mix or campesterol accelerated pupariation and increased body size. These results indicate that the dietary intake of HSM accelerates both body growth and developmental rates in Drosophila via the stimulation of cell growth and ecdysone synthesis. Additionally, nonpolar components of hempseed, such as cholesterol, might be responsible for the effects of HSM on development and reproduction.

KW - body growth

KW - cholesterol

KW - developmental rate

KW - Drosophila

KW - ecdysone

KW - hempseed meal

KW - reproduction

UR - http://www.scopus.com/inward/record.url?scp=77955015434&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955015434&partnerID=8YFLogxK

U2 - 10.1007/s10059-010-0085-0

DO - 10.1007/s10059-010-0085-0

M3 - Article

VL - 30

SP - 29

EP - 36

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 1

ER -