Abstract
Purpose: To determine the anatomic cleavage planes produced by various epikeratomes in epithelial laser in situ keratomileusis (epi-LASIK). Setting: Department of Ophthalmology, IIsan Paik Hospital, Goyang, Korea. Methods: Sixteen eyes (8 patients) were included in this study. Three epikeratomes, the Moria Epi-K, Centurion SES, and Amadeus II, were used to collect 4 epithelial flaps from 2 patients in the epi-LASIK procedure. Four epithelial flaps from 2 patients were also obtained by laser-assisted subepithelial keratectomy (LASEK). Immunohistochemical staining using monoclonal antibodies against integrin ß1, integrin ß4, laminin 5, and collagen type VII was performed. Results: Immunohistochemical staining showed expression of integrin ß1 and integrin ß4 in all epithelial flaps. In epi-LASIK, the expression of laminin 5 and collagen type VII had a linear or dotted pattern that differed based on the epikeratome used. In the epithelial flaps obtained using LASEK, the expression of laminin 5 and collagen type VII had a dotted pattern. Conclusions: Each epikeratome yielded reproducible but different cleavage planes of corneal epithelium. The results suggest that further study is needed to elucidate the wound-healing process after epi-LASIK because different cleavage planes produced by different epikeratomes may influence the process.
| Original language | English |
|---|---|
| Pages (from-to) | 2079-2084 |
| Number of pages | 6 |
| Journal | Journal of Cataract and Refractive Surgery |
| Volume | 34 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2008 Dec 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Surgery
- Ophthalmology
- Sensory Systems
Cite this
Different epithelial cleavage planes produced by various epikeratomes in epithelial laser in situ keratomileusis. / Choi, Suk Kyue; Kim, Jin Hyoung; Lee, Doh; Lee, Jae Bum; Kim, Hyo Myung; Tchah, Hung Won; Hahn, Tae Won; Joo, Mee; Ha, Chang Il.
In: Journal of Cataract and Refractive Surgery, Vol. 34, No. 12, 01.12.2008, p. 2079-2084.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Different epithelial cleavage planes produced by various epikeratomes in epithelial laser in situ keratomileusis
AU - Choi, Suk Kyue
AU - Kim, Jin Hyoung
AU - Lee, Doh
AU - Lee, Jae Bum
AU - Kim, Hyo Myung
AU - Tchah, Hung Won
AU - Hahn, Tae Won
AU - Joo, Mee
AU - Ha, Chang Il
PY - 2008/12/1
Y1 - 2008/12/1
N2 - Purpose: To determine the anatomic cleavage planes produced by various epikeratomes in epithelial laser in situ keratomileusis (epi-LASIK). Setting: Department of Ophthalmology, IIsan Paik Hospital, Goyang, Korea. Methods: Sixteen eyes (8 patients) were included in this study. Three epikeratomes, the Moria Epi-K, Centurion SES, and Amadeus II, were used to collect 4 epithelial flaps from 2 patients in the epi-LASIK procedure. Four epithelial flaps from 2 patients were also obtained by laser-assisted subepithelial keratectomy (LASEK). Immunohistochemical staining using monoclonal antibodies against integrin ß1, integrin ß4, laminin 5, and collagen type VII was performed. Results: Immunohistochemical staining showed expression of integrin ß1 and integrin ß4 in all epithelial flaps. In epi-LASIK, the expression of laminin 5 and collagen type VII had a linear or dotted pattern that differed based on the epikeratome used. In the epithelial flaps obtained using LASEK, the expression of laminin 5 and collagen type VII had a dotted pattern. Conclusions: Each epikeratome yielded reproducible but different cleavage planes of corneal epithelium. The results suggest that further study is needed to elucidate the wound-healing process after epi-LASIK because different cleavage planes produced by different epikeratomes may influence the process.
AB - Purpose: To determine the anatomic cleavage planes produced by various epikeratomes in epithelial laser in situ keratomileusis (epi-LASIK). Setting: Department of Ophthalmology, IIsan Paik Hospital, Goyang, Korea. Methods: Sixteen eyes (8 patients) were included in this study. Three epikeratomes, the Moria Epi-K, Centurion SES, and Amadeus II, were used to collect 4 epithelial flaps from 2 patients in the epi-LASIK procedure. Four epithelial flaps from 2 patients were also obtained by laser-assisted subepithelial keratectomy (LASEK). Immunohistochemical staining using monoclonal antibodies against integrin ß1, integrin ß4, laminin 5, and collagen type VII was performed. Results: Immunohistochemical staining showed expression of integrin ß1 and integrin ß4 in all epithelial flaps. In epi-LASIK, the expression of laminin 5 and collagen type VII had a linear or dotted pattern that differed based on the epikeratome used. In the epithelial flaps obtained using LASEK, the expression of laminin 5 and collagen type VII had a dotted pattern. Conclusions: Each epikeratome yielded reproducible but different cleavage planes of corneal epithelium. The results suggest that further study is needed to elucidate the wound-healing process after epi-LASIK because different cleavage planes produced by different epikeratomes may influence the process.
UR - http://www.scopus.com/inward/record.url?scp=56549123701&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=56549123701&partnerID=8YFLogxK
U2 - 10.1016/j.jcrs.2008.08.027
DO - 10.1016/j.jcrs.2008.08.027
M3 - Article
C2 - 19027563
AN - SCOPUS:56549123701
VL - 34
SP - 2079
EP - 2084
JO - Journal of Cataract and Refractive Surgery
JF - Journal of Cataract and Refractive Surgery
SN - 0886-3350
IS - 12
ER -