Different expression of vascular endothelial growth factor and pigment epithelium-derived factor between diabetic and non-diabetic epiretinal membranes

Dong Heun Nam, Jaeryung Oh, Joon Ho Roh, Kuhl Huh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose: To determine whether there is a difference in protein levels of expression of vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF), platelet-derived growth factor (PDGF) and transforming growth factor β1 (TGF-β1) between diabetic and non-diabetic epiretinal membranes (ERMs). Methods: ERMs and vitreous were surgically removed from the eyes of 8 patients with proliferative diabetic retinopathy and from 6 patients with proliferative vitreoretinopathy. Concentrations of VEGF, PEDF, PDGF and TGF-β1 were investigated by an enzyme-linked immunosorbent assay. Results: The concentrations of VEGF were higher in ERMs from patients with diabetes (9.2 ± 8.0 pg/μg protein) compared to those without diabetes (1.2 ± 3.0 pg/μg protein; p = 0.026), while the concentrations of PEDF were higher in ERMs from patients without diabetes (7.5 ± 1.5 ng/μg protein) compared to those with diabetes (3.5 ± 1.5 ng/μg protein; p = 0.002). In addition, the VEGF-to-PEDF ratio was higher in diabetic ERMs (2.80 ± 1.83) than in non-diabetic ones (0.17 ± 0.09; p = 0.019). However, the concentrations of PDGF and TGF-β1 in membranes were not different between patients with diabetes and those without diabetes. Conclusion: This study found the difference in protein levels of expression of VEGF and PEDF between diabetic and non-diabetic ERMs. These results suggest that a disturbance in the balance between angiogenic and anti-angiogenic factors may play an important role in angiogenesis in diabetic ERMs.

Original languageEnglish
Pages (from-to)188-191
Number of pages4
JournalOphthalmologica
Volume223
Issue number3
DOIs
Publication statusPublished - 2009 May

Keywords

  • Angiogenesis
  • Diabetic retinopathy
  • Epiretinal membrane
  • Pigment epithelium-derived factor
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

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