Different gamma-glutamyl transpeptidase mRNAs are expressed in human liver and kidney

A. Pawlak, S. J. Wu, F. Bulle, A. Suzuki, N. Chikhi, N. Ferry, Ja-Hyun Baik, S. Siegrist, G. Guellaen

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In human, the two subunits of gamma-glutamyl transpeptidase (CGT) arise from a common precursor encoded by a multigene family. Until now, a single specific coding sequence for this precursor (type I) has been identified in human placenta and liver. In the present study, we have isolated from a human kidney cDNA library, a GGT specific clone (0.8 Kb). The sequence of which (type II) i) covers the carboxy terminal part of the GGT precursor, ii) exhibits 22 point mutations and a 30 bp deletion as compared to the type I GGT sequence. The sequencing of a human genomic clone reveals that this type II GGT mRNA is encoded by a different gene than the type I GGT mRNA. Both type I and type II GGT mRNAs are expressed in human liver, while almost exclusively type II GGT mRNA is detected in human kidney.

Original languageEnglish
Pages (from-to)912-918
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume164
Issue number2
Publication statusPublished - 1989 Jan 1
Externally publishedYes

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gamma-Glutamyltransferase
Liver
Kidney
Messenger RNA
Clone Cells
Gene Library
Genes
Multigene Family
Point Mutation
Placenta

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Pawlak, A., Wu, S. J., Bulle, F., Suzuki, A., Chikhi, N., Ferry, N., ... Guellaen, G. (1989). Different gamma-glutamyl transpeptidase mRNAs are expressed in human liver and kidney. Biochemical and Biophysical Research Communications, 164(2), 912-918.

Different gamma-glutamyl transpeptidase mRNAs are expressed in human liver and kidney. / Pawlak, A.; Wu, S. J.; Bulle, F.; Suzuki, A.; Chikhi, N.; Ferry, N.; Baik, Ja-Hyun; Siegrist, S.; Guellaen, G.

In: Biochemical and Biophysical Research Communications, Vol. 164, No. 2, 01.01.1989, p. 912-918.

Research output: Contribution to journalArticle

Pawlak, A, Wu, SJ, Bulle, F, Suzuki, A, Chikhi, N, Ferry, N, Baik, J-H, Siegrist, S & Guellaen, G 1989, 'Different gamma-glutamyl transpeptidase mRNAs are expressed in human liver and kidney', Biochemical and Biophysical Research Communications, vol. 164, no. 2, pp. 912-918.
Pawlak A, Wu SJ, Bulle F, Suzuki A, Chikhi N, Ferry N et al. Different gamma-glutamyl transpeptidase mRNAs are expressed in human liver and kidney. Biochemical and Biophysical Research Communications. 1989 Jan 1;164(2):912-918.
Pawlak, A. ; Wu, S. J. ; Bulle, F. ; Suzuki, A. ; Chikhi, N. ; Ferry, N. ; Baik, Ja-Hyun ; Siegrist, S. ; Guellaen, G. / Different gamma-glutamyl transpeptidase mRNAs are expressed in human liver and kidney. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 164, No. 2. pp. 912-918.
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