Different gamma-glutamyl transpeptidase mRNAs are expressed in human liver and kidney

André Pawlak; Shi Jun Wu; Frédérique Bulle; Atsuo Suzuki; Naima Chikhi; Nicolas Ferry; Ja Hyun Baik; Sylvie Siegrist; Georges Guellaën

doi:10.1016/0006-291X(89)91545-3

Different gamma-glutamyl transpeptidase mRNAs are expressed in human liver and kidney

André Pawlak, Shi Jun Wu, Frédérique Bulle, Atsuo Suzuki, Naima Chikhi, Nicolas Ferry, Ja Hyun Baik, Sylvie Siegrist, Georges Guellaën

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

In human, the two subunits of gamma-glutamyl transpeptidase (GGT) arise from a common precursor encoded by a multigene family. Until now, a single specific coding sequence for this precursor (type I) has been identified in human placenta and liver. In the present study, we have isolated from a human kidney cDNA library, a GGT specific clone (0.8 Kb). The sequence of which (type II) i) covers the carboxy terminal part of the GGT precursor, ii) exhibits 22 point mutations and a 30 bp deletion as compared to the type I GGT sequence. The sequencing of a human genomic clone reveals that this type II GGT mRNA is encoded by a different gene than the type I GGT mRNA. Both type I and type II GGT mRNAs are expressed in human liver, while almost exclusively type II GGT mRNA is detected in human kidney.

Original language	English
Pages (from-to)	912-918
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	164
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(89)91545-3
Publication status	Published - 1989 Oct 31
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(89)91545-3

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title = "Different gamma-glutamyl transpeptidase mRNAs are expressed in human liver and kidney",

abstract = "In human, the two subunits of gamma-glutamyl transpeptidase (GGT) arise from a common precursor encoded by a multigene family. Until now, a single specific coding sequence for this precursor (type I) has been identified in human placenta and liver. In the present study, we have isolated from a human kidney cDNA library, a GGT specific clone (0.8 Kb). The sequence of which (type II) i) covers the carboxy terminal part of the GGT precursor, ii) exhibits 22 point mutations and a 30 bp deletion as compared to the type I GGT sequence. The sequencing of a human genomic clone reveals that this type II GGT mRNA is encoded by a different gene than the type I GGT mRNA. Both type I and type II GGT mRNAs are expressed in human liver, while almost exclusively type II GGT mRNA is detected in human kidney.",

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note = "Funding Information: Acknowledgments - This work was funded by the Mutuelle G&n&ale de 1'Education Nationale and by the Institut National de la Sante et de la Recherche Mbdicale. S.J.Wu and A. Suzuki were recipients of fellowships from the Fondation pour la Recherche M&dicale. We thank Dr. Pitot (University of",

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doi = "10.1016/0006-291X(89)91545-3",

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AU - Pawlak, André

AU - Wu, Shi Jun

AU - Bulle, Frédérique

AU - Suzuki, Atsuo

AU - Chikhi, Naima

AU - Ferry, Nicolas

AU - Baik, Ja Hyun

AU - Siegrist, Sylvie

AU - Guellaën, Georges

N1 - Funding Information: Acknowledgments - This work was funded by the Mutuelle G&n&ale de 1'Education Nationale and by the Institut National de la Sante et de la Recherche Mbdicale. S.J.Wu and A. Suzuki were recipients of fellowships from the Fondation pour la Recherche M&dicale. We thank Dr. Pitot (University of

PY - 1989/10/31

Y1 - 1989/10/31

N2 - In human, the two subunits of gamma-glutamyl transpeptidase (GGT) arise from a common precursor encoded by a multigene family. Until now, a single specific coding sequence for this precursor (type I) has been identified in human placenta and liver. In the present study, we have isolated from a human kidney cDNA library, a GGT specific clone (0.8 Kb). The sequence of which (type II) i) covers the carboxy terminal part of the GGT precursor, ii) exhibits 22 point mutations and a 30 bp deletion as compared to the type I GGT sequence. The sequencing of a human genomic clone reveals that this type II GGT mRNA is encoded by a different gene than the type I GGT mRNA. Both type I and type II GGT mRNAs are expressed in human liver, while almost exclusively type II GGT mRNA is detected in human kidney.

AB - In human, the two subunits of gamma-glutamyl transpeptidase (GGT) arise from a common precursor encoded by a multigene family. Until now, a single specific coding sequence for this precursor (type I) has been identified in human placenta and liver. In the present study, we have isolated from a human kidney cDNA library, a GGT specific clone (0.8 Kb). The sequence of which (type II) i) covers the carboxy terminal part of the GGT precursor, ii) exhibits 22 point mutations and a 30 bp deletion as compared to the type I GGT sequence. The sequencing of a human genomic clone reveals that this type II GGT mRNA is encoded by a different gene than the type I GGT mRNA. Both type I and type II GGT mRNAs are expressed in human liver, while almost exclusively type II GGT mRNA is detected in human kidney.

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Different gamma-glutamyl transpeptidase mRNAs are expressed in human liver and kidney

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