Different types of multiple-synapse boutons in the cerebellar cortex between physically enriched and ataxic mutant mice

Hyun Wook Kim, Seunghak Oh, Seung Hwan Lee, Sanghoon Lee, Ji Eun Na, Kea Joo Lee, Im Joo Rhyu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Experience-dependent synapse remodeling is associated with information storage in the nervous system. Neuronal synapses show alteration in various neurological and cognitive disorders in their structure and function. At the ultrastructural level, parallel fiber boutons contacting multiple spines of Purkinje cells in the cerebellar cortex are commonly observed in physiologically enriched animals as well as pathological ataxic mutants. However, the dendritic origin of those spines on parallel fiber multiple-synapse boutons (MSBs) has been poorly understood. Here, we investigated this issue by 3-dimensional ultrastructural analysis to determine synaptic connectivity of MSBs in both mice housed in physically enriched environment and cerebellar ataxic mutants. Our results demonstrated that environmental enrichment selectively induced MSBs to contact spines from the same parent dendrite, indicating focal strengthening of synapse through the simultaneous activation of two adjacent spines. In contrast, ataxic mutants displaying impaired motor coordination had significantly more MSBs involving spines originating from different neighboring dendrites compared to both wild-type and environmentally enriched animals, suggesting that compromising multiple synapse formation may lead to abnormal motor behavior in the mutant mice. These findings propose that environmental stimulation in normal animals mainly involves the refinement of preexisting synaptic networks, whereas pathological ataxic conditions may results from less-selective but compromising multiple synaptic formation. This study underscores that different types of multiple synapse boutons may have disparate effects on cerebellar synaptic transmission.

Original languageEnglish
Pages (from-to)25-32
Number of pages8
JournalMicroscopy Research and Technique
Volume82
Issue number1
DOIs
Publication statusPublished - 2019 Jan 1

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synapses
Cerebellar Cortex
cortexes
Synapses
mice
Animals
spine
Spine
Fibers
Neurology
animals
Chemical activation
dendrites
Dendrites
Data storage equipment
nervous system
fibers
Information Storage and Retrieval
Purkinje Cells
dimensional analysis

Keywords

  • dendritic spine
  • environmental enrichment
  • parallel fiber
  • Purkinje cell
  • synapse

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Instrumentation
  • Medical Laboratory Technology

Cite this

Different types of multiple-synapse boutons in the cerebellar cortex between physically enriched and ataxic mutant mice. / Kim, Hyun Wook; Oh, Seunghak; Lee, Seung Hwan; Lee, Sanghoon; Na, Ji Eun; Lee, Kea Joo; Rhyu, Im Joo.

In: Microscopy Research and Technique, Vol. 82, No. 1, 01.01.2019, p. 25-32.

Research output: Contribution to journalArticle

Kim, Hyun Wook ; Oh, Seunghak ; Lee, Seung Hwan ; Lee, Sanghoon ; Na, Ji Eun ; Lee, Kea Joo ; Rhyu, Im Joo. / Different types of multiple-synapse boutons in the cerebellar cortex between physically enriched and ataxic mutant mice. In: Microscopy Research and Technique. 2019 ; Vol. 82, No. 1. pp. 25-32.
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