Differential effect of chlorine on the oxidative stress generation in dormant and active cells within colony biofilm

Jaeeun Kim, Hee Jin Park, Joon Hee Lee, Ji Sook Hahn, Man Bock Gu, Jeyong Yoon

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In an effort to better control bacterial biofilm, we examined the effects of various oxidative antimicrobial chemicals including silver, paraquat, hydrogen peroxide, and chlorine depending on the physiological status of cells in biofilm. The metabolically heterogeneous cells within colony biofilm were physically fractionated and the oxidative stress generated in each fraction was monitored by soxS and oxyS promoter reporter systems. Chlorine induced soxS to a greater degree in the dormant cells than active cells of biofilm. In addition, chlorine-dependent induction of soxS was more prominent in aerobically grown cells compared with anaerobically grown cells. On the contrary, the soxS induction by other chemicals such as paraquat and silver, and the oxyS induction by hydrogen peroxide were higher in active biofilm cells and aerobically grown cells. Our results suggest that chlorine might generate strong oxidative stress by direct modification of the 2Fe-2S cluster in an O2-independent manner, which provides the molecular basis of our previous report showing that chlorine has a more efficient killing effect on dormant cells in biofilm and cells grown under unaerobic conditions. This study shows that chlorine may be particularly promising for the control of anaerobic bacteria and biofilm where dormant cells are hard to control.

Original languageEnglish
Pages (from-to)5252-5259
Number of pages8
JournalWater Research
Volume43
Issue number20
DOIs
Publication statusPublished - 2009 Dec 1

Fingerprint

Oxidative stress
Chlorine
Biofilms
biofilm
chlorine
Oxidative Stress
paraquat
Hydrogen peroxide
hydrogen peroxide
silver
Silver
Paraquat
Hydrogen Peroxide
oxidative stress
effect
Bacteria
Anaerobic Bacteria
Cells
bacterium

Keywords

  • Biofilm control
  • Chlorine
  • Oxidative stress
  • Water distribution system

ASJC Scopus subject areas

  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution
  • Ecological Modelling

Cite this

Differential effect of chlorine on the oxidative stress generation in dormant and active cells within colony biofilm. / Kim, Jaeeun; Park, Hee Jin; Lee, Joon Hee; Hahn, Ji Sook; Gu, Man Bock; Yoon, Jeyong.

In: Water Research, Vol. 43, No. 20, 01.12.2009, p. 5252-5259.

Research output: Contribution to journalArticle

Kim, Jaeeun ; Park, Hee Jin ; Lee, Joon Hee ; Hahn, Ji Sook ; Gu, Man Bock ; Yoon, Jeyong. / Differential effect of chlorine on the oxidative stress generation in dormant and active cells within colony biofilm. In: Water Research. 2009 ; Vol. 43, No. 20. pp. 5252-5259.
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