Abstract
The present study was undertaken to characterize homocysteic acid (HCA)- and cysteic acid (CA)-mediated formation of inositol phosphates (InsP) in primary culture of rat cerebellar granule cells. HCA and CA stimulated InsP formation in a dose-dependent manner, which was prevented by the N-methyl-D- aspartate (NMDA) receptor antagonist D,L-2-amino-5-phosphopentanoic acid (APV). CA-, but not HCA-, mediated InsP formation was in part prevented by the metabotropic glutamate receptor antagonist α-methyl-4- carboxyphenylglycine ((±)-MCPG). Both HCA- and CA-mediated increases in intracellular calcium concentration were completely blocked by APV, but were not altered by (±)-MCPG. CA-mediated InsP formation was in part prevented by removal of endogenous glutamate. In contrast, the glutamate transport blocker L-aspartic acid-β-hydroxamate synergistically increased CA responses. These data indicate that in cerebellar granule cells HCA mediates InsP formation wholly by activating NMDA receptor. In contrast, CA stimulates InsP formation by activating both NMDA receptor and metabotropic glutamate receptor, and in part by releasing endogenous glutamate into extracellular milieu.
| Original language | English |
|---|---|
| Pages (from-to) | 41-48 |
| Number of pages | 8 |
| Journal | Korean Journal of Physiology and Pharmacology |
| Volume | 2 |
| Issue number | 1 |
| Publication status | Published - 1998 Jan 1 |
| Externally published | Yes |
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Keywords
- [Ca]i
- Cerebellar granule cell
- Cysteic acid
- Homocysteic acid
- Inositol phosphates
- Metabotropic glutamate (mGlu) receptor
- N-methyl-D-aspartate (NMDA) receptor
- Primary culture
ASJC Scopus subject areas
- Physiology
- Pharmacology
Cite this
Differential effect of homocysteic acid and cysteic acid on changes of inositol phosphates and [Ca2+]i in rat cerebellar granule cells. / Kim, Won-Ki; Pae, Y. S.
In: Korean Journal of Physiology and Pharmacology, Vol. 2, No. 1, 01.01.1998, p. 41-48.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential effect of homocysteic acid and cysteic acid on changes of inositol phosphates and [Ca2+]i in rat cerebellar granule cells
AU - Kim, Won-Ki
AU - Pae, Y. S.
PY - 1998/1/1
Y1 - 1998/1/1
N2 - The present study was undertaken to characterize homocysteic acid (HCA)- and cysteic acid (CA)-mediated formation of inositol phosphates (InsP) in primary culture of rat cerebellar granule cells. HCA and CA stimulated InsP formation in a dose-dependent manner, which was prevented by the N-methyl-D- aspartate (NMDA) receptor antagonist D,L-2-amino-5-phosphopentanoic acid (APV). CA-, but not HCA-, mediated InsP formation was in part prevented by the metabotropic glutamate receptor antagonist α-methyl-4- carboxyphenylglycine ((±)-MCPG). Both HCA- and CA-mediated increases in intracellular calcium concentration were completely blocked by APV, but were not altered by (±)-MCPG. CA-mediated InsP formation was in part prevented by removal of endogenous glutamate. In contrast, the glutamate transport blocker L-aspartic acid-β-hydroxamate synergistically increased CA responses. These data indicate that in cerebellar granule cells HCA mediates InsP formation wholly by activating NMDA receptor. In contrast, CA stimulates InsP formation by activating both NMDA receptor and metabotropic glutamate receptor, and in part by releasing endogenous glutamate into extracellular milieu.
AB - The present study was undertaken to characterize homocysteic acid (HCA)- and cysteic acid (CA)-mediated formation of inositol phosphates (InsP) in primary culture of rat cerebellar granule cells. HCA and CA stimulated InsP formation in a dose-dependent manner, which was prevented by the N-methyl-D- aspartate (NMDA) receptor antagonist D,L-2-amino-5-phosphopentanoic acid (APV). CA-, but not HCA-, mediated InsP formation was in part prevented by the metabotropic glutamate receptor antagonist α-methyl-4- carboxyphenylglycine ((±)-MCPG). Both HCA- and CA-mediated increases in intracellular calcium concentration were completely blocked by APV, but were not altered by (±)-MCPG. CA-mediated InsP formation was in part prevented by removal of endogenous glutamate. In contrast, the glutamate transport blocker L-aspartic acid-β-hydroxamate synergistically increased CA responses. These data indicate that in cerebellar granule cells HCA mediates InsP formation wholly by activating NMDA receptor. In contrast, CA stimulates InsP formation by activating both NMDA receptor and metabotropic glutamate receptor, and in part by releasing endogenous glutamate into extracellular milieu.
KW - [Ca]i
KW - Cerebellar granule cell
KW - Cysteic acid
KW - Homocysteic acid
KW - Inositol phosphates
KW - Metabotropic glutamate (mGlu) receptor
KW - N-methyl-D-aspartate (NMDA) receptor
KW - Primary culture
UR - http://www.scopus.com/inward/record.url?scp=0031922057&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031922057&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0031922057
VL - 2
SP - 41
EP - 48
JO - Korean Journal of Physiology and Pharmacology
JF - Korean Journal of Physiology and Pharmacology
SN - 1226-4512
IS - 1
ER -