Differential expression of D-type G1 cyclins during mouse development and liver regeneration in vivo

Woong Sun, Dae Kee Lee, Chung Choo Lee, Kyungjin Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

D-type G1 cyclins are the primary cell cycle regulators of G1/S transition in eukaryotic cells, and are differentially expressed in a variety of cell lines in vitro. Little is known, however, about the expression pat terns of D-type G1 cyclins in normal mouse in vivo. Thus, in the present study, tissue-specific expressions of cyclin D1 and D3 genes were examined in several tissues derived from adult male mice, and stage-specific expression of cyclin genes was studied in brain, liver, and kidney of developing mice from embryonic day 13 to postnatal day 11. Cell cycle-dependent expression of cyclins was also examined in regenerating livers following partial hepatectomy. Our results indicate that (1) cyclins D1 and D3 are expressed in a tissue-specific manner, with cyclin D1 being highly expressed in kidney and D3 in thymus; (2) cyclin D3 mRNA is abundantly expressed in young proliferating tissues and is gradually reduced during development, whereas cyclin D1 mRNA fluctuates during development; and (3) compensatory regeneration of liver induces cyclin D1 gene expression 12 hr after partial hepatectomy, and cyclin D3 gene expression from 36 to 42 hr (at the time of G1/S transition). In conclusion, this study indicates that cyclin D1 and D3 genes are differentially expressed in vivo in a tissue-specific, developmental stage-dependent, and cell cycle-dependent manner.

Original languageEnglish
Pages (from-to)414-420
Number of pages7
JournalMolecular Reproduction and Development
Volume43
Issue number4
DOIs
Publication statusPublished - 1996 Apr 1
Externally publishedYes

Fingerprint

Cyclin D3
Cyclin D
Liver Regeneration
bcl-1 Genes
Cyclin D1
Cell Cycle
Cyclins
Hepatectomy
Gene Expression
Charadriiformes
Kidney
Messenger RNA
Liver
Eukaryotic Cells
Thymus Gland
Cell Line
Brain

Keywords

  • Cyclin D1
  • Cyclin D3
  • Liver regeneration
  • Postnatal mouse development

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Differential expression of D-type G1 cyclins during mouse development and liver regeneration in vivo. / Sun, Woong; Lee, Dae Kee; Lee, Chung Choo; Kim, Kyungjin.

In: Molecular Reproduction and Development, Vol. 43, No. 4, 01.04.1996, p. 414-420.

Research output: Contribution to journalArticle

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