Differential expression of secreted phosphoprotein 1 in response to estradiol-17β and in ovarian tumors in chickens

Whasun Lim, Wooyoung Jeong, Jinyoung Kim, Hakhyun Ka, Fuller W. Bazer, Jae Yong Han, Gwonhwa Song

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Secreted phosphoprotein 1 (SPP1), a highly phosphorylated protein containing a polyaspartic acid sequence and a conserved RGD motif, plays important roles in physiological processes such as inflammatory responses, calcification, organ development, immune cell function and carcinogenesis. Results of the present study indicate expression of SPP1 mRNA in various organs such as oviduct, small intestine and kidney from chickens, particularly in the glandular epithelium (GE) of the shell gland and, to a lesser extent, in luminal epithelium (LE) of the infundibulum and magnum, and GE of the isthmus of the oviduct. We determined that DES (diethylstilbestrol, a synthetic nonsteroidal estrogen) decreases SPP1 expression in the oviduct and that SPP1 mRNA and protein are significantly more abundant in GE of ovarian endometrioid carcinoma, but not the other cancerous and normal ovaries of hens. Further, microRNA-140 was discovered to influence SPP1 expression via its 3'-UTR which suggests that post-transcriptional regulation influences SPP1 expression in chickens. Collectively, results of this study indicate that SPP1 is novel in that its expression is down-regulated by estrogen in epithelial cells of the chicken oviduct and that it is up-regulated in chicken ovarian endometrioid tumor that could be used for monitoring effects of therapies for this disease in laying hens.

Original languageEnglish
Pages (from-to)494-500
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume422
Issue number3
DOIs
Publication statusPublished - 2012 Jun 8
Externally publishedYes

Fingerprint

Osteopontin
Tumors
Chickens
Estradiol
Oviducts
Epithelium
Neoplasms
Non-Steroidal Estrogens
Estradiol Congeners
Physiological Phenomena
Endometrioid Carcinoma
Messenger RNA
Diethylstilbestrol
Conserved Sequence
3' Untranslated Regions
Pituitary Gland
MicroRNAs
Small Intestine
Ovary
Estrogens

Keywords

  • Cancer
  • Chicken
  • DES
  • MicroRNA
  • Ovary
  • Oviduct
  • SPP1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Differential expression of secreted phosphoprotein 1 in response to estradiol-17β and in ovarian tumors in chickens. / Lim, Whasun; Jeong, Wooyoung; Kim, Jinyoung; Ka, Hakhyun; Bazer, Fuller W.; Han, Jae Yong; Song, Gwonhwa.

In: Biochemical and Biophysical Research Communications, Vol. 422, No. 3, 08.06.2012, p. 494-500.

Research output: Contribution to journalArticle

Lim, Whasun ; Jeong, Wooyoung ; Kim, Jinyoung ; Ka, Hakhyun ; Bazer, Fuller W. ; Han, Jae Yong ; Song, Gwonhwa. / Differential expression of secreted phosphoprotein 1 in response to estradiol-17β and in ovarian tumors in chickens. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 422, No. 3. pp. 494-500.
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