Differential expression of thymic DNA repair genes in low-dose-rate irradiated AKR/J mice

Jin Jong Bong, Yu Mi Kang, Suk Chul Shin, Seung Jin Choi, Kyung-Mi Lee, Hee Sun Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We previously determined that AKR/J mice housed in a low-dose-rate (LDR) (137Cs, 0.7 mGy/h, 2.1 Gy) γ-irradiation facility developed less spontaneous thymic lymphoma and survived longer than those receiving sham or high-dose-rate (HDR) (137Cs, 0.8 Gy/min, 4.5 Gy) radiation. Interestingly, histopathological analysis showed a mild lymphomagenesis in the thymus of LDR-irradiated mice. Therefore, in this study, we investigated whether LDR irradiation could trigger the expression of thymic genes involved in the DNA repair process of AKR/J mice. The enrichment analysis of Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways showed immune response, nucleosome organization, and the peroxisome proliferator-activated receptors signaling pathway in LDR-irradiated mice. Our microarray analysis and quantitative polymerase chain reaction data demonstrated that mRNA levels of Lig4 and RRM2 were specifically elevated in AKR/J mice at 130 days after the start of LDR irradiation. Furthermore, transcriptional levels of H2AX and ATM, proteins known to recruit DNA repair factors, were also shown to be upregulated. These data suggest that LDR irradiation could trigger specific induction of DNA repair-associated genes in an attempt to repair damaged DNA during tumor progression, which in turn contributed to the decreased incidence of lymphoma and increased survival. Overall, we identified specific DNA repair genes in LDR-irradiated AKR/J mice.

Original languageEnglish
Pages (from-to)271-279
Number of pages9
JournalJournal of Veterinary Science
Volume14
Issue number3
DOIs
Publication statusPublished - 2013 Oct 16

Keywords

  • AKR/J mice
  • DNA repair genes
  • Low-dose-rate radiation
  • Thymic lymphoma

ASJC Scopus subject areas

  • veterinary(all)

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