Differential expression of toll-like receptors 2 and 4 in rat middle ear

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Abstract

Objective: The epithelial cells of the middle ear and Eustachian tube must maintain an adequate mucosal defense system against various antigenic stimuli. Since toll-like receptors (TLRs) are known to play a critical role in mucosal defense, we investigated their expression in the mucosa of the tubotympanum, nasopharynx, and oral cavity of the rat. Method: The expression of TLR2 and TLR4 was examined in the mucosa of the tubotympanum, nasopharynx and oral cavity of the rat using real time RT-PCR and Western blot analysis. Results: Transcripts for TLR2 and TLR4 were detected in the mucosa of the tubotympanum, nasopharynx, and oral cavity of the rat. The expression of TLR2 and TLR4 in the middle ear was increased more than in the other anatomical areas. Differential expression of these molecules at the protein level was confirmed by Western blot analysis. Conclusions: Diverse expression of TLR2 and TLR4 in different parts of the tubotympanum and upper aerodigestive tract suggests region-specific functional modulation of the innate immune system. Differential expression of subtypes of the TLR in the normal physiology of the tubotympanum and upper aerodigestive tract also suggests that they may play a role in the pathophysiology of otitis media.

Original languageEnglish
Pages (from-to)821-824
Number of pages4
JournalInternational Journal of Pediatric Otorhinolaryngology
Volume73
Issue number6
DOIs
Publication statusPublished - 2009 Jun 1

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Toll-Like Receptor 2
Toll-Like Receptor 4
Nasopharynx
Middle Ear
Mouth
Mucous Membrane
Toll-Like Receptors
Western Blotting
Eustachian Tube
Otitis Media
Real-Time Polymerase Chain Reaction
Immune System
Epithelial Cells
Proteins

Keywords

  • Innate immunity
  • Mucosal immunity
  • Otitis media
  • Toll-like receptor

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Pediatrics, Perinatology, and Child Health

Cite this

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title = "Differential expression of toll-like receptors 2 and 4 in rat middle ear",
abstract = "Objective: The epithelial cells of the middle ear and Eustachian tube must maintain an adequate mucosal defense system against various antigenic stimuli. Since toll-like receptors (TLRs) are known to play a critical role in mucosal defense, we investigated their expression in the mucosa of the tubotympanum, nasopharynx, and oral cavity of the rat. Method: The expression of TLR2 and TLR4 was examined in the mucosa of the tubotympanum, nasopharynx and oral cavity of the rat using real time RT-PCR and Western blot analysis. Results: Transcripts for TLR2 and TLR4 were detected in the mucosa of the tubotympanum, nasopharynx, and oral cavity of the rat. The expression of TLR2 and TLR4 in the middle ear was increased more than in the other anatomical areas. Differential expression of these molecules at the protein level was confirmed by Western blot analysis. Conclusions: Diverse expression of TLR2 and TLR4 in different parts of the tubotympanum and upper aerodigestive tract suggests region-specific functional modulation of the innate immune system. Differential expression of subtypes of the TLR in the normal physiology of the tubotympanum and upper aerodigestive tract also suggests that they may play a role in the pathophysiology of otitis media.",
keywords = "Innate immunity, Mucosal immunity, Otitis media, Toll-like receptor",
author = "Jae-Jun Song and Jae-Gu Cho and Jeong-Soo Woo and Lee, {Heung Man} and Hwang, {Soon Jae} and Sungwon Chae",
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}

TY - JOUR

T1 - Differential expression of toll-like receptors 2 and 4 in rat middle ear

AU - Song, Jae-Jun

AU - Cho, Jae-Gu

AU - Woo, Jeong-Soo

AU - Lee, Heung Man

AU - Hwang, Soon Jae

AU - Chae, Sungwon

PY - 2009/6/1

Y1 - 2009/6/1

N2 - Objective: The epithelial cells of the middle ear and Eustachian tube must maintain an adequate mucosal defense system against various antigenic stimuli. Since toll-like receptors (TLRs) are known to play a critical role in mucosal defense, we investigated their expression in the mucosa of the tubotympanum, nasopharynx, and oral cavity of the rat. Method: The expression of TLR2 and TLR4 was examined in the mucosa of the tubotympanum, nasopharynx and oral cavity of the rat using real time RT-PCR and Western blot analysis. Results: Transcripts for TLR2 and TLR4 were detected in the mucosa of the tubotympanum, nasopharynx, and oral cavity of the rat. The expression of TLR2 and TLR4 in the middle ear was increased more than in the other anatomical areas. Differential expression of these molecules at the protein level was confirmed by Western blot analysis. Conclusions: Diverse expression of TLR2 and TLR4 in different parts of the tubotympanum and upper aerodigestive tract suggests region-specific functional modulation of the innate immune system. Differential expression of subtypes of the TLR in the normal physiology of the tubotympanum and upper aerodigestive tract also suggests that they may play a role in the pathophysiology of otitis media.

AB - Objective: The epithelial cells of the middle ear and Eustachian tube must maintain an adequate mucosal defense system against various antigenic stimuli. Since toll-like receptors (TLRs) are known to play a critical role in mucosal defense, we investigated their expression in the mucosa of the tubotympanum, nasopharynx, and oral cavity of the rat. Method: The expression of TLR2 and TLR4 was examined in the mucosa of the tubotympanum, nasopharynx and oral cavity of the rat using real time RT-PCR and Western blot analysis. Results: Transcripts for TLR2 and TLR4 were detected in the mucosa of the tubotympanum, nasopharynx, and oral cavity of the rat. The expression of TLR2 and TLR4 in the middle ear was increased more than in the other anatomical areas. Differential expression of these molecules at the protein level was confirmed by Western blot analysis. Conclusions: Diverse expression of TLR2 and TLR4 in different parts of the tubotympanum and upper aerodigestive tract suggests region-specific functional modulation of the innate immune system. Differential expression of subtypes of the TLR in the normal physiology of the tubotympanum and upper aerodigestive tract also suggests that they may play a role in the pathophysiology of otitis media.

KW - Innate immunity

KW - Mucosal immunity

KW - Otitis media

KW - Toll-like receptor

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