Abstract
Objectives.: To gain a better understanding of the genes involved in the pathogenesis of gestational trophoblastic diseases, we evaluated the genome-wide expression levels of genes in complete hydatidiform mole (H-mole) as compared to normal placenta using cDNA microarray technique. Methods.: The expression profiles of complete H-mole tissues were compared with those of normal placenta using cDNA microarray technique. The data obtained from 10,305 human genes were normalized by the print-tip-based LOWESS method. Significance analysis of microarray (SAM) was used to identify genes with statistically significant changes in expression. The expression levels of genes which showed significant differences between normal early placenta and complete H-mole tissues were further confirmed by RT-PCR. Results.: A cDNA microarray analysis consisting of 10,305 human genes revealed significant changes in the expression of 213 genes, with 91 genes being upregulated and 122 being downregulated. SAM revealed significant changes in gene expression, including those associated with signal transduction, cell structure, transcription, and apoptosis. Further RT-PCR analysis of altered gene expression in mole tissues supported the microarray analysis results. We confirmed the upregulation of TLE4, CAPZA1, PRSS25, RNF130, and USP1 in complete H-mole tissues. Moreover, our study provides the first evidence that ELK3, LAMA3, LNK, STAT2, and TNFRSF25 are downregulated in complete H-mole compared to normal early placenta tissues. Conclusions.: These findings provide a large body of information regarding gene expression profiles associated with complete H-mole tumorigenesis and allow the identification of potential targets for tumor prevention or therapy.
| Original language | English |
|---|---|
| Pages (from-to) | 654-660 |
| Number of pages | 7 |
| Journal | Gynecologic Oncology |
| Volume | 103 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2006 Nov 1 |
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Keywords
- cDNA microarray
- Complete hydatidiform mole
- Expression profiling
ASJC Scopus subject areas
- Obstetrics and Gynaecology
- Oncology
Cite this
Differential expression profiling of genes in a complete hydatidiform mole using cDNA microarray analysis. / Kim, Seung Jo; Lee, Sun Young; Lee, Chan; Kim, Inho; An, Hee Jung; Kim, Ji Young; Baek, Kwang Hyun; Kim, Eun Joong; Kim, Jung Mogg; Lee, Jung Bok; Lee, Jae Won; Jung, Woon Won; Chun, Taehoon; Oh, Yu Kyoung.
In: Gynecologic Oncology, Vol. 103, No. 2, 01.11.2006, p. 654-660.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential expression profiling of genes in a complete hydatidiform mole using cDNA microarray analysis
AU - Kim, Seung Jo
AU - Lee, Sun Young
AU - Lee, Chan
AU - Kim, Inho
AU - An, Hee Jung
AU - Kim, Ji Young
AU - Baek, Kwang Hyun
AU - Kim, Eun Joong
AU - Kim, Jung Mogg
AU - Lee, Jung Bok
AU - Lee, Jae Won
AU - Jung, Woon Won
AU - Chun, Taehoon
AU - Oh, Yu Kyoung
PY - 2006/11/1
Y1 - 2006/11/1
N2 - Objectives.: To gain a better understanding of the genes involved in the pathogenesis of gestational trophoblastic diseases, we evaluated the genome-wide expression levels of genes in complete hydatidiform mole (H-mole) as compared to normal placenta using cDNA microarray technique. Methods.: The expression profiles of complete H-mole tissues were compared with those of normal placenta using cDNA microarray technique. The data obtained from 10,305 human genes were normalized by the print-tip-based LOWESS method. Significance analysis of microarray (SAM) was used to identify genes with statistically significant changes in expression. The expression levels of genes which showed significant differences between normal early placenta and complete H-mole tissues were further confirmed by RT-PCR. Results.: A cDNA microarray analysis consisting of 10,305 human genes revealed significant changes in the expression of 213 genes, with 91 genes being upregulated and 122 being downregulated. SAM revealed significant changes in gene expression, including those associated with signal transduction, cell structure, transcription, and apoptosis. Further RT-PCR analysis of altered gene expression in mole tissues supported the microarray analysis results. We confirmed the upregulation of TLE4, CAPZA1, PRSS25, RNF130, and USP1 in complete H-mole tissues. Moreover, our study provides the first evidence that ELK3, LAMA3, LNK, STAT2, and TNFRSF25 are downregulated in complete H-mole compared to normal early placenta tissues. Conclusions.: These findings provide a large body of information regarding gene expression profiles associated with complete H-mole tumorigenesis and allow the identification of potential targets for tumor prevention or therapy.
AB - Objectives.: To gain a better understanding of the genes involved in the pathogenesis of gestational trophoblastic diseases, we evaluated the genome-wide expression levels of genes in complete hydatidiform mole (H-mole) as compared to normal placenta using cDNA microarray technique. Methods.: The expression profiles of complete H-mole tissues were compared with those of normal placenta using cDNA microarray technique. The data obtained from 10,305 human genes were normalized by the print-tip-based LOWESS method. Significance analysis of microarray (SAM) was used to identify genes with statistically significant changes in expression. The expression levels of genes which showed significant differences between normal early placenta and complete H-mole tissues were further confirmed by RT-PCR. Results.: A cDNA microarray analysis consisting of 10,305 human genes revealed significant changes in the expression of 213 genes, with 91 genes being upregulated and 122 being downregulated. SAM revealed significant changes in gene expression, including those associated with signal transduction, cell structure, transcription, and apoptosis. Further RT-PCR analysis of altered gene expression in mole tissues supported the microarray analysis results. We confirmed the upregulation of TLE4, CAPZA1, PRSS25, RNF130, and USP1 in complete H-mole tissues. Moreover, our study provides the first evidence that ELK3, LAMA3, LNK, STAT2, and TNFRSF25 are downregulated in complete H-mole compared to normal early placenta tissues. Conclusions.: These findings provide a large body of information regarding gene expression profiles associated with complete H-mole tumorigenesis and allow the identification of potential targets for tumor prevention or therapy.
KW - cDNA microarray
KW - Complete hydatidiform mole
KW - Expression profiling
UR - http://www.scopus.com/inward/record.url?scp=33750987910&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750987910&partnerID=8YFLogxK
U2 - 10.1016/j.ygyno.2006.05.015
DO - 10.1016/j.ygyno.2006.05.015
M3 - Article
C2 - 16797685
AN - SCOPUS:33750987910
VL - 103
SP - 654
EP - 660
JO - Gynecologic Oncology
JF - Gynecologic Oncology
SN - 0090-8258
IS - 2
ER -