Differential G protein coupling preference of mammalian and nonmammalian gonadotropin-releasing hormone receptors

Da Young Oh, Li Wang, Ryun Sup Ahn, Jae-Yong Park, Jae Young Seong, Hyuk Bang Kwon

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Recently, we have identified three distinct types of gonadotropin- releasing hormone receptor (GnRHR) in the bullfrog (designated bfGnRHR-1, bfGnRHR-2, and bfGnRHR-3). In the present study, we compared G protein coupling preference of mammalian and nonmammalian GnRHRs. In a transient expression system, stimulation of either bfGnRHRs or rat GnRHR by GnRH significantly increased both inositol phosphates (IP) and cAMP productions, but ratios of IP to cAMP induction levels were quite different among the receptors, indicating differential G protein coupling preference. Using cAMP-dependent protein kinase A (PKA)-specific (CRE-luc) or protein kinase C (PKC)-specific reporter (c-fos-luc) systems, we further examined Gs and Gq/11 coupling preference of these GnRHRs. Since activities of CRE-luc and c-fos-luc were highly dependent on cell types, GnRH-induced CRE-luc or c-fos-luc activity was normalized by forskolin-induced CRE-luc or 12-O-tetradecanoylphenol-13- acetate (TPA)-induced c-fos-luc activity, respectively. This normalized result indicated that bfGnRHR-2 couples to Gs more actively than G q/11, while bfGnRHR-1 and -3 couple to Gs and G q/11 with similar strength. However, the rat GnRHR appeared to couple to Gq/11 more efficiently than Gs. This study was further confirmed by an experiment in which GnRH augmented CRE-driven luciferase activity in αT3-1 cells when CRE-luc was cotransfected with bfGnRHRs but not with vehicle or rat GnRHR. Collectively, these results indicate that mammalian and nonmammalian GnRHRs may induce diverse cellular and physiological responses through differential activation of PKA and PKC signaling pathways.

Original languageEnglish
Pages (from-to)89-98
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume205
Issue number1-2
DOIs
Publication statusPublished - 2003 Jul 31
Externally publishedYes

Fingerprint

LHRH Receptors
GTP-Binding Proteins
Cyclic AMP-Dependent Protein Kinases
Gonadotropin-Releasing Hormone
Rats
Inositol Phosphates
Protein Kinase C
Rana catesbeiana
Colforsin
Luciferases
Acetates
Chemical activation
Experiments

Keywords

  • Bullfrog
  • Coupling preference
  • G protein
  • Gonadotropin-releasing hormone receptor
  • Protein kinase A
  • Protein kinase C
  • Signal transduction

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Differential G protein coupling preference of mammalian and nonmammalian gonadotropin-releasing hormone receptors. / Oh, Da Young; Wang, Li; Ahn, Ryun Sup; Park, Jae-Yong; Seong, Jae Young; Kwon, Hyuk Bang.

In: Molecular and Cellular Endocrinology, Vol. 205, No. 1-2, 31.07.2003, p. 89-98.

Research output: Contribution to journalArticle

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