Differential gene expression profiles of the olfactory bulb after nasal obstruction in neonatal rats

Gan Undram Semjidsuren; Gi Jung Im; Sang Hag Lee; Se Jin Park; Ho Yeon Hwang; Hak Hyun Jung

doi:10.1016/j.otohns.2007.12.036

Differential gene expression profiles of the olfactory bulb after nasal obstruction in neonatal rats

Gan Undram Semjidsuren, Gi Jung Im, Sang Hag Lee, Se Jin Park, Ho Yeon Hwang, Hak Hyun Jung

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Objective: Microarray technique is a useful tool to identify functional gene candidates. In this study, we evaluated the gene expression profiles in the olfactory bulbs of normal rats and naris-occluded rats using the gene microarray technique. Study Design and Methods: To induce atrophic change in the olfactory bulb, we performed a unilateral nasal obstruction by electronic cauterization on postnatal day 1 rats. Differential gene expression profiles of the nasal obstruction group and the normal control group at postnatal day 35 were analyzed with a DNA microarray. Results: Microarray revealed 41 genes that were upregulated at least 2-fold in the nasal obstruction group compared with the control group. Among these upregulated genes, increased expression levels of 20 functional genes were confirmed by semiquantitative reverse transcription-polymerase chain reaction. Conclusion: This study examines candidate genes associated with the development, apoptosis, and signal transduction of the olfactory bulb. These results may explain the fact that blockage of airflow by inflammation and nasal polyps causes deprivation of olfactory functions in vivo.

Original language	English
Pages (from-to)	648-654
Number of pages	7
Journal	Otolaryngology - Head and Neck Surgery
Volume	138
Issue number	5
DOIs	https://doi.org/10.1016/j.otohns.2007.12.036
Publication status	Published - 2008 May
Externally published	Yes

ASJC Scopus subject areas

Surgery
Otorhinolaryngology

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10.1016/j.otohns.2007.12.036

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title = "Differential gene expression profiles of the olfactory bulb after nasal obstruction in neonatal rats",

abstract = "Objective: Microarray technique is a useful tool to identify functional gene candidates. In this study, we evaluated the gene expression profiles in the olfactory bulbs of normal rats and naris-occluded rats using the gene microarray technique. Study Design and Methods: To induce atrophic change in the olfactory bulb, we performed a unilateral nasal obstruction by electronic cauterization on postnatal day 1 rats. Differential gene expression profiles of the nasal obstruction group and the normal control group at postnatal day 35 were analyzed with a DNA microarray. Results: Microarray revealed 41 genes that were upregulated at least 2-fold in the nasal obstruction group compared with the control group. Among these upregulated genes, increased expression levels of 20 functional genes were confirmed by semiquantitative reverse transcription-polymerase chain reaction. Conclusion: This study examines candidate genes associated with the development, apoptosis, and signal transduction of the olfactory bulb. These results may explain the fact that blockage of airflow by inflammation and nasal polyps causes deprivation of olfactory functions in vivo.",

author = "Semjidsuren, {Gan Undram} and Im, {Gi Jung} and Lee, {Sang Hag} and Park, {Se Jin} and Hwang, {Ho Yeon} and Jung, {Hak Hyun}",

note = "Funding Information: This study was supported by the Brain Korea Project 21 and the Institutes of Communication Disorder at Korea University.",

year = "2008",

month = may,

doi = "10.1016/j.otohns.2007.12.036",

language = "English",

volume = "138",

pages = "648--654",

journal = "Otolaryngology - Head and Neck Surgery (United States)",

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T1 - Differential gene expression profiles of the olfactory bulb after nasal obstruction in neonatal rats

AU - Semjidsuren, Gan Undram

AU - Im, Gi Jung

AU - Lee, Sang Hag

AU - Park, Se Jin

AU - Hwang, Ho Yeon

AU - Jung, Hak Hyun

N1 - Funding Information: This study was supported by the Brain Korea Project 21 and the Institutes of Communication Disorder at Korea University.

PY - 2008/5

Y1 - 2008/5

N2 - Objective: Microarray technique is a useful tool to identify functional gene candidates. In this study, we evaluated the gene expression profiles in the olfactory bulbs of normal rats and naris-occluded rats using the gene microarray technique. Study Design and Methods: To induce atrophic change in the olfactory bulb, we performed a unilateral nasal obstruction by electronic cauterization on postnatal day 1 rats. Differential gene expression profiles of the nasal obstruction group and the normal control group at postnatal day 35 were analyzed with a DNA microarray. Results: Microarray revealed 41 genes that were upregulated at least 2-fold in the nasal obstruction group compared with the control group. Among these upregulated genes, increased expression levels of 20 functional genes were confirmed by semiquantitative reverse transcription-polymerase chain reaction. Conclusion: This study examines candidate genes associated with the development, apoptosis, and signal transduction of the olfactory bulb. These results may explain the fact that blockage of airflow by inflammation and nasal polyps causes deprivation of olfactory functions in vivo.

AB - Objective: Microarray technique is a useful tool to identify functional gene candidates. In this study, we evaluated the gene expression profiles in the olfactory bulbs of normal rats and naris-occluded rats using the gene microarray technique. Study Design and Methods: To induce atrophic change in the olfactory bulb, we performed a unilateral nasal obstruction by electronic cauterization on postnatal day 1 rats. Differential gene expression profiles of the nasal obstruction group and the normal control group at postnatal day 35 were analyzed with a DNA microarray. Results: Microarray revealed 41 genes that were upregulated at least 2-fold in the nasal obstruction group compared with the control group. Among these upregulated genes, increased expression levels of 20 functional genes were confirmed by semiquantitative reverse transcription-polymerase chain reaction. Conclusion: This study examines candidate genes associated with the development, apoptosis, and signal transduction of the olfactory bulb. These results may explain the fact that blockage of airflow by inflammation and nasal polyps causes deprivation of olfactory functions in vivo.

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DO - 10.1016/j.otohns.2007.12.036

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AN - SCOPUS:42249087317

SN - 0194-5998

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JO - Otolaryngology - Head and Neck Surgery (United States)

JF - Otolaryngology - Head and Neck Surgery (United States)

IS - 5

ER -

Differential gene expression profiles of the olfactory bulb after nasal obstruction in neonatal rats

Abstract

ASJC Scopus subject areas

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