Differential proteomic analysis of secreted proteins from cutinase-producing Bacillus sp. SB-007

Yeon Hee Ban, Miri Jeon, Jihee Yoon, Jae Min Park, Hyun Ju Um, Dae Soon Kim, Seung Ki Jung, Keun Young Kim, Jeewon Lee, Jiho Min, Yang Hoon Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Bacillus sp. SB-007 was isolated from pea leaves harvested from the southwestern parts of South Korea through screening on a minimal medium containing 0.2% purified cutin for its ability to induce the cutinase production. However, no cutinase was produced when it was grown in a minimal medium containing 0.2% glucose. A proteomic approach was applied to separate and characterize these differentially secreted proteins. The expression level of 83 extracellular proteins of the cutinase-producing Bacillus sp. strain SB-007 incubated in a cutinase-induced medium increased significantly as compared with that cultured in a non cutinase-induced medium containing glucose. The extracellular proteome of Bacillus sp. SB-007 includes proteins from different functional classes, such as enzymes for the degradation of various macromolecules, proteins involved in energy metabolism, sporulation, transport/binding proteins and lipoproteins, stress inducible proteins, several cellular molecule biosynthetic pathways and catabolism, and some proteins with an as yet unknown function. In addition, the two protein spots showed little similarities with the known lipolytic enzymes in the database. These secreted proteome analysis results are expected to be useful in improving the Bacillus strains for the production of industrial cutinases.

Original languageEnglish
Pages (from-to)191-201
Number of pages11
JournalPlant Pathology Journal
Volume24
Issue number2
DOIs
Publication statusPublished - 2008

Keywords

  • Bacillus sp
  • Cutinase activity
  • Secreted proteome profiling
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Agronomy and Crop Science

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