Differential regulation of CC chemokine receptors by 9-cis retinoic acid in the human mast cell line, HMC-1

Jesang Ko, Chi Young Yun, Ji Sook Lee, Dong Hee Kim, Ji Eun Yuk, In Sik Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Mast cells are well known as effector cells in a variety of inflammatory diseases, including asthma as well as other allergic disorders. The precise role of 9-cis retinoic acid (9CRA) in mast cells is not understood despite the accepted fact that 9CRA regulates inflammatory responses and neutrophil differentiation. In this study, we investigated the effects of 9CRA on the expression of CC chemokine receptors in the human mast cell line, HMC-1. 9CRA selectively inhibits the CCR2 mRNA level and increases the CCR3 mRNA level in both a time and dose dependent manner. Other CC chemokine receptors, including CCR1, CCR4 and CCR5 are not altered by treatment with 9CRA. Both TNF-α and LPS, known pro-inflammatory molecules, have no effect on mRNA levels of CC chemokine receptors. For surface expression, 9CRA decreased the CCR2 level but had no effect on the CCR3 level. 9CRA inhibited the chemotactic activity in response to the CCR2-dependent chemokine, MCP-1/CCL2 but not in response to CCR3-specific chemokine, eotaxin/CCL11. 9CRA decreased spontaneous homotype clustering. Therefore, our results demonstrate that 9CRA differentially decreases both CCR2 expression and chemotactic ability of HMC-1 cells, and may regulate the inflammatory effects of mast cells.

Original languageEnglish
Pages (from-to)1293-1300
Number of pages8
JournalLife Sciences
Volume79
Issue number13
DOIs
Publication statusPublished - 2006 Aug 22

Keywords

  • CC chemokine receptor
  • Inflammation
  • Mast cells
  • Retinoic acid

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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