Differentially expressed proteins of mesenchymal stem cells derived from human cord blood (hUCB) during osteogenic differentiation

Ji Soo Kim, Hyun Kyung Lee, Mi Ryung Kim, Pan Kyeom Kim, Chan Wha Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Multipotent mesenchymal stem cells (MSCs) derived from human umbilical cord blood (hUCB) represent promising candidates for the development of future cellular therapy strategies. MSCs have been found to be able to differentiate into various tissues. One of the primary limitations in our understanding of the biology of human MSCs is the absence of prospective markers required for the monitoring of lineage-specific differentiation. hUCB-derived MSCs have been found to have significantly greater osteogenic potential. In this study, we focused on proteins that were differentially expressed during osteogenic differentiation of hUCB-MSCs. And we analyzed the protein expression inherent to osteogenic differentiation by two-dimensional gel electrophoresis, ESI-Q-TOF, and Western blotting. Eleven differentially expressed spots were observed between the two groups (before and after differentiation) on the 2-DE map. These might also be proved as useful cytosolic biomarker proteins for osteogenesis, and might be employed in quality control of osteoblasts in cell-therapy applications.

Original languageEnglish
Pages (from-to)2309-2317
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number9
DOIs
Publication statusPublished - 2008

Keywords

  • Human umbillical cord blood
  • Mesenchymal stem cells
  • Osteogenesis
  • Proteomics

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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