Abstract
Multipotent mesenchymal stem cells (MSCs) derived from human umbilical cord blood (hUCB) represent promising candidates for the development of future cellular therapy strategies. MSCs have been found to be able to differentiate into various tissues. One of the primary limitations in our understanding of the biology of human MSCs is the absence of prospective markers required for the monitoring of lineage-specific differentiation. hUCB-derived MSCs have been found to have significantly greater osteogenic potential. In this study, we focused on proteins that were differentially expressed during osteogenic differentiation of hUCB-MSCs. And we analyzed the protein expression inherent to osteogenic differentiation by two-dimensional gel electrophoresis, ESI-Q-TOF, and Western blotting. Eleven differentially expressed spots were observed between the two groups (before and after differentiation) on the 2-DE map. These might also be proved as useful cytosolic biomarker proteins for osteogenesis, and might be employed in quality control of osteoblasts in cell-therapy applications.
| Original language | English |
|---|---|
| Pages (from-to) | 2309-2317 |
| Number of pages | 9 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 72 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2008 Oct 1 |
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Keywords
- Human umbillical cord blood
- Mesenchymal stem cells
- Osteogenesis
- Proteomics
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Applied Microbiology and Biotechnology
- Analytical Chemistry
- Organic Chemistry
Cite this
Differentially expressed proteins of mesenchymal stem cells derived from human cord blood (hUCB) during osteogenic differentiation. / Kim, Ji Soo; Lee, Hyun Kyung; Kim, Mi Ryung; Kim, Pan Kyeom; Kim, Chan Wha.
In: Bioscience, Biotechnology and Biochemistry, Vol. 72, No. 9, 01.10.2008, p. 2309-2317.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differentially expressed proteins of mesenchymal stem cells derived from human cord blood (hUCB) during osteogenic differentiation
AU - Kim, Ji Soo
AU - Lee, Hyun Kyung
AU - Kim, Mi Ryung
AU - Kim, Pan Kyeom
AU - Kim, Chan Wha
PY - 2008/10/1
Y1 - 2008/10/1
N2 - Multipotent mesenchymal stem cells (MSCs) derived from human umbilical cord blood (hUCB) represent promising candidates for the development of future cellular therapy strategies. MSCs have been found to be able to differentiate into various tissues. One of the primary limitations in our understanding of the biology of human MSCs is the absence of prospective markers required for the monitoring of lineage-specific differentiation. hUCB-derived MSCs have been found to have significantly greater osteogenic potential. In this study, we focused on proteins that were differentially expressed during osteogenic differentiation of hUCB-MSCs. And we analyzed the protein expression inherent to osteogenic differentiation by two-dimensional gel electrophoresis, ESI-Q-TOF, and Western blotting. Eleven differentially expressed spots were observed between the two groups (before and after differentiation) on the 2-DE map. These might also be proved as useful cytosolic biomarker proteins for osteogenesis, and might be employed in quality control of osteoblasts in cell-therapy applications.
AB - Multipotent mesenchymal stem cells (MSCs) derived from human umbilical cord blood (hUCB) represent promising candidates for the development of future cellular therapy strategies. MSCs have been found to be able to differentiate into various tissues. One of the primary limitations in our understanding of the biology of human MSCs is the absence of prospective markers required for the monitoring of lineage-specific differentiation. hUCB-derived MSCs have been found to have significantly greater osteogenic potential. In this study, we focused on proteins that were differentially expressed during osteogenic differentiation of hUCB-MSCs. And we analyzed the protein expression inherent to osteogenic differentiation by two-dimensional gel electrophoresis, ESI-Q-TOF, and Western blotting. Eleven differentially expressed spots were observed between the two groups (before and after differentiation) on the 2-DE map. These might also be proved as useful cytosolic biomarker proteins for osteogenesis, and might be employed in quality control of osteoblasts in cell-therapy applications.
KW - Human umbillical cord blood
KW - Mesenchymal stem cells
KW - Osteogenesis
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=52649132443&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=52649132443&partnerID=8YFLogxK
U2 - 10.1271/bbb.80224
DO - 10.1271/bbb.80224
M3 - Article
VL - 72
SP - 2309
EP - 2317
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 9
ER -