Differentiation of Acremonium chrysogenum M35 in submerged culture with glass beads or silicone rubbers

Hwan Hyo Lee, Hyun Yong Shin, Eun Ji Kim, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


In this study, we investigated the effects of glass beads and silicone rubbers on the differentiation and morphological changes of A. chrysogenum M35 in submerged culture. Differentiation in the center of the cell pellets was improved by the addition of glass beads or silicone rubbers to the primary medium. The fragmentation rate constant (kfrag), which is used to estimate the tensile strength of fungal hyphae, was increased by more than 40% in baffled flasks containing glass beads. The maximum fragmentation rate was also increased by 48% when silicone rubbers were added to a 5 L bioreactor containing the culture. During the cultivation in the main medium with 6 glass beads, the value of the fractal dimension increased by about 8% when it was compared with baffled flasks without glass beads. Additionally, the number of arthrospores and the dry cell weight were increased by more than 10% in baffled flasks containing beads. The degree of roundness, which is the ratio of the object area to the longest Feret diameter, was decreased from 0.85 at day 1 to 0.77 at day 5. The differentiation of A. chrysogenum M35 was also supposedly closely related with the enlargement of the cell surfaces. Taken together, these results indicate that complex changes in morphology resulted in increased cell growth and differentiation in the culture broth containing glass beads and silicone rubbers.

Original languageEnglish
Pages (from-to)734-738
Number of pages5
JournalJournal of Microbiology
Issue number6
Publication statusPublished - 2010


  • A. chrysogenum
  • fractal dimension
  • glass bead
  • tensile strength

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology


Dive into the research topics of 'Differentiation of Acremonium chrysogenum M35 in submerged culture with glass beads or silicone rubbers'. Together they form a unique fingerprint.

Cite this