Abstract
In this study, we investigated the application of human embryonic stem cells (hESCs) to neural tissue engineering. hESCs were differentiated to neural progenitor cells and cultured on poly(lactic-co-glycolic acid) (pLGA) microfibers. PLGA microfibers were prepared by using a microfluidic spinning system and aligned in parallel manner by winding the nascent fibers around a rotating frame. Neural progenitor cells were prepared by differentiating embryoid bodies (EBs). Neural progenitors cultured on the PLGA fibers were assessed for further proliferation and differentiation. To verify the expression of neural progenitor markers, cells were immunostained with neuronal specific antibodies. After six-days in culture, neuronal protein expression was confirmed with neuron specific beta III tubulin antibody (Tujl) and glial fibrillary acidic protein (GFAP). After 20-days, cells were immunostained with microfilament associated protein (MAP) and neurofilament. The neurites of differentiated cells cultured for 20 days elongated along the direction of the PLGA microfibers. This result shows the possibility of using PLGA microfibers for neural tissue regeneration as a guidance cue for hESC derived cells.
| Original language | English |
|---|---|
| Title of host publication | Bioengineering, Proceedings of the Northeast Conference |
| DOIs | |
| Publication status | Published - 2009 Sep 21 |
| Event | IEEE 35th Annual Northeast Bioengineering Conference, NEBEC 2009 - Boston, MA, United States Duration: 2009 Apr 3 → 2009 Apr 5 |
Other
| Other | IEEE 35th Annual Northeast Bioengineering Conference, NEBEC 2009 |
|---|---|
| Country | United States |
| City | Boston, MA |
| Period | 09/4/3 → 09/4/5 |
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ASJC Scopus subject areas
- Bioengineering
Cite this
Differentiation of human neural progenitor cells on PLGA microfibers. / Hwang, C. M.; Kim, S. K.; Kim, L. H.; Khademhosseini, A.; Lee, Sang Hoon.
Bioengineering, Proceedings of the Northeast Conference. 2009. 4967758.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
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TY - GEN
T1 - Differentiation of human neural progenitor cells on PLGA microfibers
AU - Hwang, C. M.
AU - Kim, S. K.
AU - Kim, L. H.
AU - Khademhosseini, A.
AU - Lee, Sang Hoon
PY - 2009/9/21
Y1 - 2009/9/21
N2 - In this study, we investigated the application of human embryonic stem cells (hESCs) to neural tissue engineering. hESCs were differentiated to neural progenitor cells and cultured on poly(lactic-co-glycolic acid) (pLGA) microfibers. PLGA microfibers were prepared by using a microfluidic spinning system and aligned in parallel manner by winding the nascent fibers around a rotating frame. Neural progenitor cells were prepared by differentiating embryoid bodies (EBs). Neural progenitors cultured on the PLGA fibers were assessed for further proliferation and differentiation. To verify the expression of neural progenitor markers, cells were immunostained with neuronal specific antibodies. After six-days in culture, neuronal protein expression was confirmed with neuron specific beta III tubulin antibody (Tujl) and glial fibrillary acidic protein (GFAP). After 20-days, cells were immunostained with microfilament associated protein (MAP) and neurofilament. The neurites of differentiated cells cultured for 20 days elongated along the direction of the PLGA microfibers. This result shows the possibility of using PLGA microfibers for neural tissue regeneration as a guidance cue for hESC derived cells.
AB - In this study, we investigated the application of human embryonic stem cells (hESCs) to neural tissue engineering. hESCs were differentiated to neural progenitor cells and cultured on poly(lactic-co-glycolic acid) (pLGA) microfibers. PLGA microfibers were prepared by using a microfluidic spinning system and aligned in parallel manner by winding the nascent fibers around a rotating frame. Neural progenitor cells were prepared by differentiating embryoid bodies (EBs). Neural progenitors cultured on the PLGA fibers were assessed for further proliferation and differentiation. To verify the expression of neural progenitor markers, cells were immunostained with neuronal specific antibodies. After six-days in culture, neuronal protein expression was confirmed with neuron specific beta III tubulin antibody (Tujl) and glial fibrillary acidic protein (GFAP). After 20-days, cells were immunostained with microfilament associated protein (MAP) and neurofilament. The neurites of differentiated cells cultured for 20 days elongated along the direction of the PLGA microfibers. This result shows the possibility of using PLGA microfibers for neural tissue regeneration as a guidance cue for hESC derived cells.
UR - http://www.scopus.com/inward/record.url?scp=70349098160&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349098160&partnerID=8YFLogxK
U2 - 10.1109/NEBC.2009.4967758
DO - 10.1109/NEBC.2009.4967758
M3 - Conference contribution
AN - SCOPUS:70349098160
SN - 9781424443628
BT - Bioengineering, Proceedings of the Northeast Conference
ER -