Diffusion-Induced Hydrophilic Conversion of Polydimethylsiloxane/Block-Type Phospholipid Polymer Hybrid Substrate for Temporal Cell-Adhesive Surface

Ji-Hun Seo, Kazuhiko Ishihara

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this study, diffusion-induced hydrophobic-hydrophilic conversion of the surface of the cross-linked polydimethylsiloxane (PDMS) substrate was realized by employing a simple swelling-deswelling process of PDMS substrate in a block-type polymer solution with the aim of development of a temporal cell-adhesive substrate. The ABA block-type polymer composed of poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) segment and PDMS segment with over 70% of dimethylsiloxane unit composition could be successfully incorporated in the PDMS substrate during the swelling-deswelling process to prepare the PDMS/phospholipid block copolymer hybrid substrates. During the aging process of the PDMS substrate for 4 days in aqueous medium, its surface property changed gradually from hydrophobic to hydrophilic. X-ray photoelectron spectroscopy and atomic force microscopy data provided strong evidence that the time-dependent hydrophilic conversion of the PDMS/block-type phospholipid polymer hybrid substrate was induced by the diffusion of the hydrophilic PMPC segment in the block-type polymer to be tethered on the substrate. During the hydrophilic conversion process, surface-adsorbed fibronectin was gradually desorbed from the substrate surface, and this resulted in successful detachment of two-dimensional connected cell crowds.

Original languageEnglish
Pages (from-to)21839-21846
Number of pages8
JournalACS Applied Materials and Interfaces
Volume8
Issue number33
DOIs
Publication statusPublished - 2016 Aug 24

Fingerprint

Phospholipids
Polydimethylsiloxane
Adhesives
Polymers
Substrates
Swelling
Dimethylpolysiloxanes
baysilon
Polymer solutions
Fibronectins
Block copolymers
Surface properties
Atomic force microscopy
X ray photoelectron spectroscopy
Aging of materials
Chemical analysis

Keywords

  • cell adhesion
  • hybrid material
  • poly(2-methacryloyloxyethyl phosphorylcholine)
  • polydimethylsiloxane
  • swelling-deswelling process

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

@article{97a2af39ccef4f6182f6f766e1b1e5d8,
title = "Diffusion-Induced Hydrophilic Conversion of Polydimethylsiloxane/Block-Type Phospholipid Polymer Hybrid Substrate for Temporal Cell-Adhesive Surface",
abstract = "In this study, diffusion-induced hydrophobic-hydrophilic conversion of the surface of the cross-linked polydimethylsiloxane (PDMS) substrate was realized by employing a simple swelling-deswelling process of PDMS substrate in a block-type polymer solution with the aim of development of a temporal cell-adhesive substrate. The ABA block-type polymer composed of poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) segment and PDMS segment with over 70{\%} of dimethylsiloxane unit composition could be successfully incorporated in the PDMS substrate during the swelling-deswelling process to prepare the PDMS/phospholipid block copolymer hybrid substrates. During the aging process of the PDMS substrate for 4 days in aqueous medium, its surface property changed gradually from hydrophobic to hydrophilic. X-ray photoelectron spectroscopy and atomic force microscopy data provided strong evidence that the time-dependent hydrophilic conversion of the PDMS/block-type phospholipid polymer hybrid substrate was induced by the diffusion of the hydrophilic PMPC segment in the block-type polymer to be tethered on the substrate. During the hydrophilic conversion process, surface-adsorbed fibronectin was gradually desorbed from the substrate surface, and this resulted in successful detachment of two-dimensional connected cell crowds.",
keywords = "cell adhesion, hybrid material, poly(2-methacryloyloxyethyl phosphorylcholine), polydimethylsiloxane, swelling-deswelling process",
author = "Ji-Hun Seo and Kazuhiko Ishihara",
year = "2016",
month = "8",
day = "24",
doi = "10.1021/acsami.6b07414",
language = "English",
volume = "8",
pages = "21839--21846",
journal = "ACS applied materials & interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "33",

}

TY - JOUR

T1 - Diffusion-Induced Hydrophilic Conversion of Polydimethylsiloxane/Block-Type Phospholipid Polymer Hybrid Substrate for Temporal Cell-Adhesive Surface

AU - Seo, Ji-Hun

AU - Ishihara, Kazuhiko

PY - 2016/8/24

Y1 - 2016/8/24

N2 - In this study, diffusion-induced hydrophobic-hydrophilic conversion of the surface of the cross-linked polydimethylsiloxane (PDMS) substrate was realized by employing a simple swelling-deswelling process of PDMS substrate in a block-type polymer solution with the aim of development of a temporal cell-adhesive substrate. The ABA block-type polymer composed of poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) segment and PDMS segment with over 70% of dimethylsiloxane unit composition could be successfully incorporated in the PDMS substrate during the swelling-deswelling process to prepare the PDMS/phospholipid block copolymer hybrid substrates. During the aging process of the PDMS substrate for 4 days in aqueous medium, its surface property changed gradually from hydrophobic to hydrophilic. X-ray photoelectron spectroscopy and atomic force microscopy data provided strong evidence that the time-dependent hydrophilic conversion of the PDMS/block-type phospholipid polymer hybrid substrate was induced by the diffusion of the hydrophilic PMPC segment in the block-type polymer to be tethered on the substrate. During the hydrophilic conversion process, surface-adsorbed fibronectin was gradually desorbed from the substrate surface, and this resulted in successful detachment of two-dimensional connected cell crowds.

AB - In this study, diffusion-induced hydrophobic-hydrophilic conversion of the surface of the cross-linked polydimethylsiloxane (PDMS) substrate was realized by employing a simple swelling-deswelling process of PDMS substrate in a block-type polymer solution with the aim of development of a temporal cell-adhesive substrate. The ABA block-type polymer composed of poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) segment and PDMS segment with over 70% of dimethylsiloxane unit composition could be successfully incorporated in the PDMS substrate during the swelling-deswelling process to prepare the PDMS/phospholipid block copolymer hybrid substrates. During the aging process of the PDMS substrate for 4 days in aqueous medium, its surface property changed gradually from hydrophobic to hydrophilic. X-ray photoelectron spectroscopy and atomic force microscopy data provided strong evidence that the time-dependent hydrophilic conversion of the PDMS/block-type phospholipid polymer hybrid substrate was induced by the diffusion of the hydrophilic PMPC segment in the block-type polymer to be tethered on the substrate. During the hydrophilic conversion process, surface-adsorbed fibronectin was gradually desorbed from the substrate surface, and this resulted in successful detachment of two-dimensional connected cell crowds.

KW - cell adhesion

KW - hybrid material

KW - poly(2-methacryloyloxyethyl phosphorylcholine)

KW - polydimethylsiloxane

KW - swelling-deswelling process

UR - http://www.scopus.com/inward/record.url?scp=84983480765&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84983480765&partnerID=8YFLogxK

U2 - 10.1021/acsami.6b07414

DO - 10.1021/acsami.6b07414

M3 - Article

VL - 8

SP - 21839

EP - 21846

JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

SN - 1944-8244

IS - 33

ER -