Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing

Patrick A. Sandoz, Ahmet F. Coskun, Aram Chung, Westbrook M. Weaver, Oladunni Adeyiga, Delaram Khodadadi, Aydogan Ozcan, Dino Di Carlo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We report a new technique to improve signal fidelity for implementing on-chip digital immunoassays running on a cell-phone. The assay targets the human immunodeficiency virus (HIV) p24 capsid protein to quantify viral load as part of a compact device for point of care (POC) testing. The sample is digitized as water-in-oil droplets and integrates advanced techniques utilizing Poisson distributions of target molecules, robust emulsion generation, and increasing sensitivity up to previously unachievable levels.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages338-340
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Fingerprint

Poisson distribution
Capsid Proteins
Emulsions
Viruses
Assays
Oils
Proteins
Molecules
Water
Testing

Keywords

  • Digital elisa
  • Fluorophore stabilization
  • Water in oil emulsion
  • Wide field of view fluorescence imaging

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Sandoz, P. A., Coskun, A. F., Chung, A., Weaver, W. M., Adeyiga, O., Khodadadi, D., ... Di Carlo, D. (2012). Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 338-340). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.

Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing. / Sandoz, Patrick A.; Coskun, Ahmet F.; Chung, Aram; Weaver, Westbrook M.; Adeyiga, Oladunni; Khodadadi, Delaram; Ozcan, Aydogan; Di Carlo, Dino.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 338-340 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sandoz, PA, Coskun, AF, Chung, A, Weaver, WM, Adeyiga, O, Khodadadi, D, Ozcan, A & Di Carlo, D 2012, Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Chemical and Biological Microsystems Society, pp. 338-340, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 12/10/28.
Sandoz PA, Coskun AF, Chung A, Weaver WM, Adeyiga O, Khodadadi D et al. Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 338-340. (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).
Sandoz, Patrick A. ; Coskun, Ahmet F. ; Chung, Aram ; Weaver, Westbrook M. ; Adeyiga, Oladunni ; Khodadadi, Delaram ; Ozcan, Aydogan ; Di Carlo, Dino. / Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 338-340 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).
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