Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing

Patrick A. Sandoz, Ahmet F. Coskun, Aram J. Chung, Westbrook M. Weaver, Oladunni Adeyiga, Delaram Khodadadi, Aydogan Ozcan, Dino Di Carlo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We report a new technique to improve signal fidelity for implementing on-chip digital immunoassays running on a cell-phone. The assay targets the human immunodeficiency virus (HIV) p24 capsid protein to quantify viral load as part of a compact device for point of care (POC) testing. The sample is digitized as water-in-oil droplets and integrates advanced techniques utilizing Poisson distributions of target molecules, robust emulsion generation, and increasing sensitivity up to previously unachievable levels.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages338-340
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Keywords

  • Digital elisa
  • Fluorophore stabilization
  • Water in oil emulsion
  • Wide field of view fluorescence imaging

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Sandoz, P. A., Coskun, A. F., Chung, A. J., Weaver, W. M., Adeyiga, O., Khodadadi, D., Ozcan, A., & Di Carlo, D. (2012). Digital readout platform for water-in-oil droplet immunoassays running on a cell-phone for point of care viral load sensing. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 338-340). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.