Dilute acid pretreatment of barley straw and its saccharification and fermentation

Sung Bong Kim, Ja Hyun Lee, Kyeong Keun Oh, Sang Jun Lee, Jin Young Lee, Jun Seok Kim, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)


In this study, the optimization of the major factors for efficient dilute acid pretreatment (DAP) of Korean barley straw was conducted by response surface method (RSM). In addition, saccharification of the optimized pretreated barley straw as well as fermentation of solubilized hemicellulose and enzymatic hydrolysates was performed for bioethanol production. The factors optimized by RSM were concentration of sulfuric acid, reaction time and temperature. Optimization experiments were carried out within the scope of 0.16 ∼ 1.84% sulfuric acid, 10 ∼ 20 min of reaction time, and 116 ∼ 183°C of temperature using a statistical program, and optimal conditions (1.16% of sulfuric acid, 16.9 min of reaction time, and 150°C) were determined based on reliable statistical indicators. The predicted value at stationary point and the experimental value were 81.38 and 80.66%, respectively. Saccharification was performed at 50°C using Celluclast (cellulase) and Novozyme 188 (β-glucosidase) as biocatalysts in an enzyme loading test. Conversion of the saccharification process was approximately 65%. In addition, fermentation of glucose after saccharification and solubilization of xylose solution by DAP were performed using Saccharomyces cerevisiae and Pichia stipitis at 30°C and 200 rpm for 12 h.

Original languageEnglish
Pages (from-to)725-732
Number of pages8
JournalBiotechnology and Bioprocess Engineering
Issue number4
Publication statusPublished - 2011 Aug


  • dilute acid pretreatment
  • fermentation
  • optimization
  • response surface methodology
  • saccharification
  • sulfuric acid

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering


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