Dioxin induces transforming growth factor-α in human keratinocytes

Eui Ju Choi, Diane G. Toscano, Judith A. Ryan, Norbert Riedel, William A. Toscano

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Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a widespread environmental toxicant, is a tumor promoter that induces hyperplasia in epithelial cells. Exposure of cultured human keratinocytes to TCDD, resulted in a time-dependent dioxin-specific Ah receptor-mediated release of transforming growth factor-α (TGF-α) into the culture medium. Cultures exposed to TCDD showed a rate of TGF-αa secretion into the medium of about 30 fmol/ml/day, as well as a 3- to 6-fold increase in TGF-α mRNA expression. Increased production of TGF-α in human keratinocytes exposed to TCDD demonstrates a modulation of autocrine regulation in those cells. These results suggest that induction of TGF-α could be an important part of the mechanism of dioxin-mediated toxicity and tumor promotion.

Original languageEnglish
Pages (from-to)9591-9597
Number of pages7
JournalJournal of Biological Chemistry
Volume266
Issue number15
Publication statusPublished - 1991 Dec 1
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Choi, E. J., Toscano, D. G., Ryan, J. A., Riedel, N., & Toscano, W. A. (1991). Dioxin induces transforming growth factor-α in human keratinocytes. Journal of Biological Chemistry, 266(15), 9591-9597.