Direct Conversion of CO2 to α-Farnesene Using Metabolically Engineered Synechococcus elongatus PCC 7942

Hyun Jeong Lee, Jiwon Lee, Sun Mi Lee, Youngsoon Um, Yunje Kim, Sang Jun Sim, Jong Il Choi, Han Min Woo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Direct conversion of carbon dioxide (CO2) to value-added chemicals by engineering of cyanobacteria has received attention as a sustainable strategy in food and chemical industries. Herein, Synechococcus elongatus PCC 7942, a model cyanobacterium, was engineered to produce α-farnesene from CO2. As a result of the lack of farnesene synthase (FS) activity in the wild-type cyanobacterium, we metabolically engineered S. elongatus PCC 7942 to express heterologous FS from either Norway spruce or apple fruit, resulting in detectable peaks of α-farnesene. To enhance α-farnesene production, an optimized methylerythritol phosphate (MEP) pathway was introduced in the farnesene-producing strain to supply farnesyl diphosphate. Subsequent cyanobacterial culture with a dodecane overlay resulted in photosynthetic production of α-farnesene (4.6 ± 0.4 mg/L in 7 days) from CO2. To the best of our knowledge, this is the first report of the photosynthetic production of α-farnesene from CO2 in the unicellular cyanobacterium S. elongatus PCC 7942.

Original languageEnglish
Pages (from-to)10424-10428
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume65
Issue number48
DOIs
Publication statusPublished - 2017 Dec 6

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Synechococcus sp. PCC 7942
Synechococcus
farnesene
Cyanobacteria
carbon dioxide
Chemical Engineering
Chemical Industry
Food Industry
Malus
Norway
Chemical industry
Fruits
Carbon Dioxide
Fruit
Phosphates
chemical industry
value added
Picea abies
food industry
engineering

Keywords

  • CO conversion
  • cyanobacteria
  • farnesene
  • metabolic engineering

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Direct Conversion of CO2 to α-Farnesene Using Metabolically Engineered Synechococcus elongatus PCC 7942. / Lee, Hyun Jeong; Lee, Jiwon; Lee, Sun Mi; Um, Youngsoon; Kim, Yunje; Sim, Sang Jun; Choi, Jong Il; Woo, Han Min.

In: Journal of Agricultural and Food Chemistry, Vol. 65, No. 48, 06.12.2017, p. 10424-10428.

Research output: Contribution to journalArticle

Lee, Hyun Jeong ; Lee, Jiwon ; Lee, Sun Mi ; Um, Youngsoon ; Kim, Yunje ; Sim, Sang Jun ; Choi, Jong Il ; Woo, Han Min. / Direct Conversion of CO2 to α-Farnesene Using Metabolically Engineered Synechococcus elongatus PCC 7942. In: Journal of Agricultural and Food Chemistry. 2017 ; Vol. 65, No. 48. pp. 10424-10428.
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