Direct fabrication of enzyme-carrying polymer nanofibers by electrospinning

Thurston E. Herricks, Sae Hoon Kim, Jungbae Kim, Dan Li, Ja Hun Kwak, Jay W. Grate, Seong H. Kim, Younan Xia

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Nanofibers consisting of enzyme-polymer composites have been prepared by directly electrospinning a solution of surfactant-stabilized enzyme and polymer in toluene. Additional treatment with glutaraldehyde could greatly stabilize the enzyme activity of the fibers, which could be maintained in a buffer under shaking conditions for more than two weeks. The nanofibers also showed great improvement in the enzyme activity over bulk films as a result of increased mass-transfer for substrate molecules to and from the enzyme reactive sites. These stable and catalytically active nanofiber-based mats were highly durable and could be easily recovered from solution, making them ideal candidates for large scale applications.

Original languageEnglish
Pages (from-to)3241-3245
Number of pages5
JournalJournal of Materials Chemistry
Volume15
Issue number31
DOIs
Publication statusPublished - 2005 Aug 21
Externally publishedYes

Fingerprint

Electrospinning
Nanofibers
enzymes
Polymers
enzyme activity
Enzymes
Enzyme activity
Fabrication
fabrication
polymers
shaking
Toluene
Glutaral
Surface-Active Agents
mass transfer
toluene
Buffers
Surface active agents
Mass transfer
buffers

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Materials Science(all)

Cite this

Herricks, T. E., Kim, S. H., Kim, J., Li, D., Kwak, J. H., Grate, J. W., ... Xia, Y. (2005). Direct fabrication of enzyme-carrying polymer nanofibers by electrospinning. Journal of Materials Chemistry, 15(31), 3241-3245. https://doi.org/10.1039/b503660g

Direct fabrication of enzyme-carrying polymer nanofibers by electrospinning. / Herricks, Thurston E.; Kim, Sae Hoon; Kim, Jungbae; Li, Dan; Kwak, Ja Hun; Grate, Jay W.; Kim, Seong H.; Xia, Younan.

In: Journal of Materials Chemistry, Vol. 15, No. 31, 21.08.2005, p. 3241-3245.

Research output: Contribution to journalArticle

Herricks, TE, Kim, SH, Kim, J, Li, D, Kwak, JH, Grate, JW, Kim, SH & Xia, Y 2005, 'Direct fabrication of enzyme-carrying polymer nanofibers by electrospinning', Journal of Materials Chemistry, vol. 15, no. 31, pp. 3241-3245. https://doi.org/10.1039/b503660g
Herricks, Thurston E. ; Kim, Sae Hoon ; Kim, Jungbae ; Li, Dan ; Kwak, Ja Hun ; Grate, Jay W. ; Kim, Seong H. ; Xia, Younan. / Direct fabrication of enzyme-carrying polymer nanofibers by electrospinning. In: Journal of Materials Chemistry. 2005 ; Vol. 15, No. 31. pp. 3241-3245.
@article{5bc4283cd4ce469fbe4ae2c496e52d49,
title = "Direct fabrication of enzyme-carrying polymer nanofibers by electrospinning",
abstract = "Nanofibers consisting of enzyme-polymer composites have been prepared by directly electrospinning a solution of surfactant-stabilized enzyme and polymer in toluene. Additional treatment with glutaraldehyde could greatly stabilize the enzyme activity of the fibers, which could be maintained in a buffer under shaking conditions for more than two weeks. The nanofibers also showed great improvement in the enzyme activity over bulk films as a result of increased mass-transfer for substrate molecules to and from the enzyme reactive sites. These stable and catalytically active nanofiber-based mats were highly durable and could be easily recovered from solution, making them ideal candidates for large scale applications.",
author = "Herricks, {Thurston E.} and Kim, {Sae Hoon} and Jungbae Kim and Dan Li and Kwak, {Ja Hun} and Grate, {Jay W.} and Kim, {Seong H.} and Younan Xia",
year = "2005",
month = "8",
day = "21",
doi = "10.1039/b503660g",
language = "English",
volume = "15",
pages = "3241--3245",
journal = "Journal of Materials Chemistry",
issn = "0959-9428",
publisher = "Royal Society of Chemistry",
number = "31",

}

TY - JOUR

T1 - Direct fabrication of enzyme-carrying polymer nanofibers by electrospinning

AU - Herricks, Thurston E.

AU - Kim, Sae Hoon

AU - Kim, Jungbae

AU - Li, Dan

AU - Kwak, Ja Hun

AU - Grate, Jay W.

AU - Kim, Seong H.

AU - Xia, Younan

PY - 2005/8/21

Y1 - 2005/8/21

N2 - Nanofibers consisting of enzyme-polymer composites have been prepared by directly electrospinning a solution of surfactant-stabilized enzyme and polymer in toluene. Additional treatment with glutaraldehyde could greatly stabilize the enzyme activity of the fibers, which could be maintained in a buffer under shaking conditions for more than two weeks. The nanofibers also showed great improvement in the enzyme activity over bulk films as a result of increased mass-transfer for substrate molecules to and from the enzyme reactive sites. These stable and catalytically active nanofiber-based mats were highly durable and could be easily recovered from solution, making them ideal candidates for large scale applications.

AB - Nanofibers consisting of enzyme-polymer composites have been prepared by directly electrospinning a solution of surfactant-stabilized enzyme and polymer in toluene. Additional treatment with glutaraldehyde could greatly stabilize the enzyme activity of the fibers, which could be maintained in a buffer under shaking conditions for more than two weeks. The nanofibers also showed great improvement in the enzyme activity over bulk films as a result of increased mass-transfer for substrate molecules to and from the enzyme reactive sites. These stable and catalytically active nanofiber-based mats were highly durable and could be easily recovered from solution, making them ideal candidates for large scale applications.

UR - http://www.scopus.com/inward/record.url?scp=23944513592&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23944513592&partnerID=8YFLogxK

U2 - 10.1039/b503660g

DO - 10.1039/b503660g

M3 - Article

AN - SCOPUS:23944513592

VL - 15

SP - 3241

EP - 3245

JO - Journal of Materials Chemistry

JF - Journal of Materials Chemistry

SN - 0959-9428

IS - 31

ER -