Direct interaction and functional coupling between voltage-gated CaV1.3 Ca2+ channel and GABAB receptor subunit 2

Hye Won Park, Hana Jung, Kee Hyun Choi, Ja-Hyun Baik, Hyewhon Rhim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Although CaV1.2 and CaV1.3 are two subtypes of L-type Ca2+ channels expressed in the CNS, functions of CaV1.3 have not been well elucidated compared to CaV1.2. Here, we found that CaV1.3-NT associates with GABABR2-CT using yeast two-hybrid, GST pull-down and co-immunoprecipitation assays. We also demonstrated co-localization of CaV1.3 and GABABR2 in HEK293 cells and cultured hippocampal neurons. Whole-cell patch-clamp and Ca2+-imaging experiments revealed that activation of GABABR increases CaV1.3 currents and intracellular Ca2+ via CaV1.3, but not CaV1.2. These results show a physical and functional interaction between CaV1.3 and GABABR, suggesting the potential pivotal roles of CaV1.3 in the CNS.

Original languageEnglish
Pages (from-to)3317-3322
Number of pages6
JournalFEBS Letters
Volume584
Issue number15
DOIs
Publication statusPublished - 2010 Aug 1

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HEK293 Cells
Clamping devices
Immunoprecipitation
Yeast
Neurons
Assays
Yeasts
Chemical activation
Imaging techniques
Electric potential
Experiments

Keywords

  • Baclofen
  • Intracellular Ca
  • L-Type Ca channels
  • Whole-cell patch-clamp

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Direct interaction and functional coupling between voltage-gated CaV1.3 Ca2+ channel and GABAB receptor subunit 2. / Park, Hye Won; Jung, Hana; Choi, Kee Hyun; Baik, Ja-Hyun; Rhim, Hyewhon.

In: FEBS Letters, Vol. 584, No. 15, 01.08.2010, p. 3317-3322.

Research output: Contribution to journalArticle

Park, Hye Won ; Jung, Hana ; Choi, Kee Hyun ; Baik, Ja-Hyun ; Rhim, Hyewhon. / Direct interaction and functional coupling between voltage-gated CaV1.3 Ca2+ channel and GABAB receptor subunit 2. In: FEBS Letters. 2010 ; Vol. 584, No. 15. pp. 3317-3322.
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