Direct interaction and functional coupling between voltage-gated CaV1.3 Ca2+ channel and GABAB receptor subunit 2

Hye Won Park, Hana Jung, Kee Hyun Choi, Ja Hyun Baik, Hyewhon Rhim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Although CaV1.2 and CaV1.3 are two subtypes of L-type Ca2+ channels expressed in the CNS, functions of CaV1.3 have not been well elucidated compared to CaV1.2. Here, we found that CaV1.3-NT associates with GABABR2-CT using yeast two-hybrid, GST pull-down and co-immunoprecipitation assays. We also demonstrated co-localization of CaV1.3 and GABABR2 in HEK293 cells and cultured hippocampal neurons. Whole-cell patch-clamp and Ca2+-imaging experiments revealed that activation of GABABR increases CaV1.3 currents and intracellular Ca2+ via CaV1.3, but not CaV1.2. These results show a physical and functional interaction between CaV1.3 and GABABR, suggesting the potential pivotal roles of CaV1.3 in the CNS.

Original languageEnglish
Pages (from-to)3317-3322
Number of pages6
JournalFEBS Letters
Volume584
Issue number15
DOIs
Publication statusPublished - 2010 Aug

Keywords

  • Baclofen
  • Intracellular Ca
  • L-Type Ca channels
  • Whole-cell patch-clamp

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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