Abstract
Raman spectroscopy has strong potential for providing noninvasive dermatological diagnosis of skin cancer. In this study, confocal Roman microscopy was applied to the dermatological diagnosis for one of the most common skin cancers, basal cell carcinoma (BCC). BCC tissues were obtained from 10 BCC patients using a routine biopsy and used for confocal Roman measurements. Autofluorescence signals from tissues, which interfere with the Roman signals, were greatly reduced using a confocal slit adjustment. Distinct Roman band differences between normal and BCC tissues for the amide I mode and the PO 2- symmetric stretching mode showed that this technique has strong potential for use as a dermatological diagnostic tool without the need for statistical treatment of spectral data. It was also possible to precisely differentiate BCC tissue from surrounding noncancerous tissue using the confocal Roman depth profiling technique. We propose that confocal Roman microscopy provides a novel method for dermatological diagnosis since direct observations of spectral differences between normal and BCC tissues are possible.
| Original language | English |
|---|---|
| Pages (from-to) | 264-272 |
| Number of pages | 9 |
| Journal | Biopolymers |
| Volume | 77 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2005 Apr 5 |
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Keywords
- Basal cell carcinoma
- Confocal Raman microscopy
- Diagnosis
- Noninvasive
- Skin cancer
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Biomaterials
- Organic Chemistry
Cite this
Direct observation of spectral differences between normal and basal cell carcinoma (BCC) tissues using confocal Raman microscopy. / Choi, Junghyun; Choo, Jaebum; Chung, Hoeil; Gweon, Dae Gab; Park, Jeunghee; Kim, Hyo Jin; Park, Sangyong; Oh, Chil Hwan.
In: Biopolymers, Vol. 77, No. 5, 05.04.2005, p. 264-272.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Direct observation of spectral differences between normal and basal cell carcinoma (BCC) tissues using confocal Raman microscopy
AU - Choi, Junghyun
AU - Choo, Jaebum
AU - Chung, Hoeil
AU - Gweon, Dae Gab
AU - Park, Jeunghee
AU - Kim, Hyo Jin
AU - Park, Sangyong
AU - Oh, Chil Hwan
PY - 2005/4/5
Y1 - 2005/4/5
N2 - Raman spectroscopy has strong potential for providing noninvasive dermatological diagnosis of skin cancer. In this study, confocal Roman microscopy was applied to the dermatological diagnosis for one of the most common skin cancers, basal cell carcinoma (BCC). BCC tissues were obtained from 10 BCC patients using a routine biopsy and used for confocal Roman measurements. Autofluorescence signals from tissues, which interfere with the Roman signals, were greatly reduced using a confocal slit adjustment. Distinct Roman band differences between normal and BCC tissues for the amide I mode and the PO 2- symmetric stretching mode showed that this technique has strong potential for use as a dermatological diagnostic tool without the need for statistical treatment of spectral data. It was also possible to precisely differentiate BCC tissue from surrounding noncancerous tissue using the confocal Roman depth profiling technique. We propose that confocal Roman microscopy provides a novel method for dermatological diagnosis since direct observations of spectral differences between normal and BCC tissues are possible.
AB - Raman spectroscopy has strong potential for providing noninvasive dermatological diagnosis of skin cancer. In this study, confocal Roman microscopy was applied to the dermatological diagnosis for one of the most common skin cancers, basal cell carcinoma (BCC). BCC tissues were obtained from 10 BCC patients using a routine biopsy and used for confocal Roman measurements. Autofluorescence signals from tissues, which interfere with the Roman signals, were greatly reduced using a confocal slit adjustment. Distinct Roman band differences between normal and BCC tissues for the amide I mode and the PO 2- symmetric stretching mode showed that this technique has strong potential for use as a dermatological diagnostic tool without the need for statistical treatment of spectral data. It was also possible to precisely differentiate BCC tissue from surrounding noncancerous tissue using the confocal Roman depth profiling technique. We propose that confocal Roman microscopy provides a novel method for dermatological diagnosis since direct observations of spectral differences between normal and BCC tissues are possible.
KW - Basal cell carcinoma
KW - Confocal Raman microscopy
KW - Diagnosis
KW - Noninvasive
KW - Skin cancer
UR - http://www.scopus.com/inward/record.url?scp=17444383471&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17444383471&partnerID=8YFLogxK
U2 - 10.1002/bip.20236
DO - 10.1002/bip.20236
M3 - Article
C2 - 15657894
AN - SCOPUS:17444383471
VL - 77
SP - 264
EP - 272
JO - Biopolymers
JF - Biopolymers
SN - 0006-3525
IS - 5
ER -