Direct observation of spectral differences between normal and basal cell carcinoma (BCC) tissues using confocal Raman microscopy

Junghyun Choi, Jaebum Choo, Hoeil Chung, Dae Gab Gweon, Jeunghee Park, Hyo Jin Kim, Sangyong Park, Chil Hwan Oh

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Raman spectroscopy has strong potential for providing noninvasive dermatological diagnosis of skin cancer. In this study, confocal Roman microscopy was applied to the dermatological diagnosis for one of the most common skin cancers, basal cell carcinoma (BCC). BCC tissues were obtained from 10 BCC patients using a routine biopsy and used for confocal Roman measurements. Autofluorescence signals from tissues, which interfere with the Roman signals, were greatly reduced using a confocal slit adjustment. Distinct Roman band differences between normal and BCC tissues for the amide I mode and the PO 2- symmetric stretching mode showed that this technique has strong potential for use as a dermatological diagnostic tool without the need for statistical treatment of spectral data. It was also possible to precisely differentiate BCC tissue from surrounding noncancerous tissue using the confocal Roman depth profiling technique. We propose that confocal Roman microscopy provides a novel method for dermatological diagnosis since direct observations of spectral differences between normal and BCC tissues are possible.

Original languageEnglish
Pages (from-to)264-272
Number of pages9
JournalBiopolymers
Volume77
Issue number5
DOIs
Publication statusPublished - 2005 Apr 5

Fingerprint

Basal Cell Carcinoma
Confocal Microscopy
Microscopic examination
Cells
Observation
Tissue
Confocal microscopy
Skin Neoplasms
Skin
Depth profiling
Raman Spectrum Analysis
Biopsy
Amides
Stretching
Raman spectroscopy

Keywords

  • Basal cell carcinoma
  • Confocal Raman microscopy
  • Diagnosis
  • Noninvasive
  • Skin cancer

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry

Cite this

Direct observation of spectral differences between normal and basal cell carcinoma (BCC) tissues using confocal Raman microscopy. / Choi, Junghyun; Choo, Jaebum; Chung, Hoeil; Gweon, Dae Gab; Park, Jeunghee; Kim, Hyo Jin; Park, Sangyong; Oh, Chil Hwan.

In: Biopolymers, Vol. 77, No. 5, 05.04.2005, p. 264-272.

Research output: Contribution to journalArticle

Choi, J, Choo, J, Chung, H, Gweon, DG, Park, J, Kim, HJ, Park, S & Oh, CH 2005, 'Direct observation of spectral differences between normal and basal cell carcinoma (BCC) tissues using confocal Raman microscopy', Biopolymers, vol. 77, no. 5, pp. 264-272. https://doi.org/10.1002/bip.20236
Choi, Junghyun ; Choo, Jaebum ; Chung, Hoeil ; Gweon, Dae Gab ; Park, Jeunghee ; Kim, Hyo Jin ; Park, Sangyong ; Oh, Chil Hwan. / Direct observation of spectral differences between normal and basal cell carcinoma (BCC) tissues using confocal Raman microscopy. In: Biopolymers. 2005 ; Vol. 77, No. 5. pp. 264-272.
@article{d08f0976ccb44f3aac7b25967503728a,
title = "Direct observation of spectral differences between normal and basal cell carcinoma (BCC) tissues using confocal Raman microscopy",
abstract = "Raman spectroscopy has strong potential for providing noninvasive dermatological diagnosis of skin cancer. In this study, confocal Roman microscopy was applied to the dermatological diagnosis for one of the most common skin cancers, basal cell carcinoma (BCC). BCC tissues were obtained from 10 BCC patients using a routine biopsy and used for confocal Roman measurements. Autofluorescence signals from tissues, which interfere with the Roman signals, were greatly reduced using a confocal slit adjustment. Distinct Roman band differences between normal and BCC tissues for the amide I mode and the PO 2- symmetric stretching mode showed that this technique has strong potential for use as a dermatological diagnostic tool without the need for statistical treatment of spectral data. It was also possible to precisely differentiate BCC tissue from surrounding noncancerous tissue using the confocal Roman depth profiling technique. We propose that confocal Roman microscopy provides a novel method for dermatological diagnosis since direct observations of spectral differences between normal and BCC tissues are possible.",
keywords = "Basal cell carcinoma, Confocal Raman microscopy, Diagnosis, Noninvasive, Skin cancer",
author = "Junghyun Choi and Jaebum Choo and Hoeil Chung and Gweon, {Dae Gab} and Jeunghee Park and Kim, {Hyo Jin} and Sangyong Park and Oh, {Chil Hwan}",
year = "2005",
month = "4",
day = "5",
doi = "10.1002/bip.20236",
language = "English",
volume = "77",
pages = "264--272",
journal = "Biopolymers",
issn = "0006-3525",
publisher = "John Wiley and Sons Inc.",
number = "5",

}

TY - JOUR

T1 - Direct observation of spectral differences between normal and basal cell carcinoma (BCC) tissues using confocal Raman microscopy

AU - Choi, Junghyun

AU - Choo, Jaebum

AU - Chung, Hoeil

AU - Gweon, Dae Gab

AU - Park, Jeunghee

AU - Kim, Hyo Jin

AU - Park, Sangyong

AU - Oh, Chil Hwan

PY - 2005/4/5

Y1 - 2005/4/5

N2 - Raman spectroscopy has strong potential for providing noninvasive dermatological diagnosis of skin cancer. In this study, confocal Roman microscopy was applied to the dermatological diagnosis for one of the most common skin cancers, basal cell carcinoma (BCC). BCC tissues were obtained from 10 BCC patients using a routine biopsy and used for confocal Roman measurements. Autofluorescence signals from tissues, which interfere with the Roman signals, were greatly reduced using a confocal slit adjustment. Distinct Roman band differences between normal and BCC tissues for the amide I mode and the PO 2- symmetric stretching mode showed that this technique has strong potential for use as a dermatological diagnostic tool without the need for statistical treatment of spectral data. It was also possible to precisely differentiate BCC tissue from surrounding noncancerous tissue using the confocal Roman depth profiling technique. We propose that confocal Roman microscopy provides a novel method for dermatological diagnosis since direct observations of spectral differences between normal and BCC tissues are possible.

AB - Raman spectroscopy has strong potential for providing noninvasive dermatological diagnosis of skin cancer. In this study, confocal Roman microscopy was applied to the dermatological diagnosis for one of the most common skin cancers, basal cell carcinoma (BCC). BCC tissues were obtained from 10 BCC patients using a routine biopsy and used for confocal Roman measurements. Autofluorescence signals from tissues, which interfere with the Roman signals, were greatly reduced using a confocal slit adjustment. Distinct Roman band differences between normal and BCC tissues for the amide I mode and the PO 2- symmetric stretching mode showed that this technique has strong potential for use as a dermatological diagnostic tool without the need for statistical treatment of spectral data. It was also possible to precisely differentiate BCC tissue from surrounding noncancerous tissue using the confocal Roman depth profiling technique. We propose that confocal Roman microscopy provides a novel method for dermatological diagnosis since direct observations of spectral differences between normal and BCC tissues are possible.

KW - Basal cell carcinoma

KW - Confocal Raman microscopy

KW - Diagnosis

KW - Noninvasive

KW - Skin cancer

UR - http://www.scopus.com/inward/record.url?scp=17444383471&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17444383471&partnerID=8YFLogxK

U2 - 10.1002/bip.20236

DO - 10.1002/bip.20236

M3 - Article

C2 - 15657894

AN - SCOPUS:17444383471

VL - 77

SP - 264

EP - 272

JO - Biopolymers

JF - Biopolymers

SN - 0006-3525

IS - 5

ER -