Direct observation of the formation of DNA triplexes by single-molecule FRET measurements

Il Buem Lee, Ja Yil Lee, Nam Kyung Lee, Seok Cheol Hong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this report we investigated the effects of various biological and chemical factors (DNA sequence, pH, ions, and molecularity) on the formation of DNA triplexes through single-molecule FRET technique. Using this method, we determined how the third strand bound to a DNA duplex and how stable the triplex structure was under various conditions. From this study, we not only verified a variety of well-known features of DNA triplex but also discovered or experimentally supported several interesting behaviors: at neutral pH, a pyrimidine-motif triplex can be formed; the parallel arrangement was not only possible but also dominant over the antiparallel arrangement for a purine-motif triplex. We demonstrated that our method is a versatile analytical tool in studying structural aspects of nucleic acids, particularly non-classical DNA structures, and provides insights into physical mechanism of such structures.

Original languageEnglish
Pages (from-to)1027-1032
Number of pages6
JournalCurrent Applied Physics
Volume12
Issue number4
DOIs
Publication statusPublished - 2012 Jul

Keywords

  • DNA triplex
  • H-DNA
  • Single-molecule FRET
  • Strand alignment
  • pH

ASJC Scopus subject areas

  • Materials Science(all)
  • Physics and Astronomy(all)

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