Abstract
In this report we investigated the effects of various biological and chemical factors (DNA sequence, pH, ions, and molecularity) on the formation of DNA triplexes through single-molecule FRET technique. Using this method, we determined how the third strand bound to a DNA duplex and how stable the triplex structure was under various conditions. From this study, we not only verified a variety of well-known features of DNA triplex but also discovered or experimentally supported several interesting behaviors: at neutral pH, a pyrimidine-motif triplex can be formed; the parallel arrangement was not only possible but also dominant over the antiparallel arrangement for a purine-motif triplex. We demonstrated that our method is a versatile analytical tool in studying structural aspects of nucleic acids, particularly non-classical DNA structures, and provides insights into physical mechanism of such structures.
Original language | English |
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Pages (from-to) | 1027-1032 |
Number of pages | 6 |
Journal | Current Applied Physics |
Volume | 12 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2012 Jul |
Keywords
- DNA triplex
- H-DNA
- Single-molecule FRET
- Strand alignment
- pH
ASJC Scopus subject areas
- Materials Science(all)
- Physics and Astronomy(all)