Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells

Kondapa Naidu Bobba, Anupama Binoy, Seyoung Koo, Divya Nedungadi, Arup Podder, Amit Sharma, Nandita Mishra, Jong Seung Kim, Sankarprasad Bhuniya

Research output: Contribution to journalArticle

Abstract

Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.

Original languageEnglish
Pages (from-to)6429-6432
Number of pages4
JournalChemical Communications
Volume55
Issue number45
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Mitochondria
Cells
2,4-Dinitrophenol
Adenosinetriphosphate
Fluorescent Dyes
Neoplasms
Adenosine Triphosphate
Mitochondrial Membrane Potential
Fluorescence
Chemical activation
Membranes
Monitoring
Therapeutics

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Bobba, K. N., Binoy, A., Koo, S., Nedungadi, D., Podder, A., Sharma, A., ... Bhuniya, S. (2019). Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells. Chemical Communications, 55(45), 6429-6432. https://doi.org/10.1039/c9cc01483g

Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells. / Bobba, Kondapa Naidu; Binoy, Anupama; Koo, Seyoung; Nedungadi, Divya; Podder, Arup; Sharma, Amit; Mishra, Nandita; Kim, Jong Seung; Bhuniya, Sankarprasad.

In: Chemical Communications, Vol. 55, No. 45, 01.01.2019, p. 6429-6432.

Research output: Contribution to journalArticle

Bobba, KN, Binoy, A, Koo, S, Nedungadi, D, Podder, A, Sharma, A, Mishra, N, Kim, JS & Bhuniya, S 2019, 'Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells', Chemical Communications, vol. 55, no. 45, pp. 6429-6432. https://doi.org/10.1039/c9cc01483g
Bobba, Kondapa Naidu ; Binoy, Anupama ; Koo, Seyoung ; Nedungadi, Divya ; Podder, Arup ; Sharma, Amit ; Mishra, Nandita ; Kim, Jong Seung ; Bhuniya, Sankarprasad. / Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells. In: Chemical Communications. 2019 ; Vol. 55, No. 45. pp. 6429-6432.
@article{f62606b7dbb04b0f8343519696fdb567,
title = "Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells",
abstract = "Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.",
author = "Bobba, {Kondapa Naidu} and Anupama Binoy and Seyoung Koo and Divya Nedungadi and Arup Podder and Amit Sharma and Nandita Mishra and Kim, {Jong Seung} and Sankarprasad Bhuniya",
year = "2019",
month = "1",
day = "1",
doi = "10.1039/c9cc01483g",
language = "English",
volume = "55",
pages = "6429--6432",
journal = "Chemical Communications",
issn = "1359-7345",
publisher = "Royal Society of Chemistry",
number = "45",

}

TY - JOUR

T1 - Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells

AU - Bobba, Kondapa Naidu

AU - Binoy, Anupama

AU - Koo, Seyoung

AU - Nedungadi, Divya

AU - Podder, Arup

AU - Sharma, Amit

AU - Mishra, Nandita

AU - Kim, Jong Seung

AU - Bhuniya, Sankarprasad

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.

AB - Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.

UR - http://www.scopus.com/inward/record.url?scp=85066451443&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85066451443&partnerID=8YFLogxK

U2 - 10.1039/c9cc01483g

DO - 10.1039/c9cc01483g

M3 - Article

VL - 55

SP - 6429

EP - 6432

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 45

ER -