Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells

Kondapa Naidu Bobba, Anupama Binoy, Seyoung Koo, Divya Nedungadi, Arup Podder, Amit Sharma, Nandita Mishra, Jong Seung Kim, Sankarprasad Bhuniya

Research output: Contribution to journalArticle

Abstract

Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.

Original languageEnglish
Pages (from-to)6429-6432
Number of pages4
JournalChemical Communications
Volume55
Issue number45
DOIs
Publication statusPublished - 2019 Jan 1

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ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Bobba, K. N., Binoy, A., Koo, S., Nedungadi, D., Podder, A., Sharma, A., Mishra, N., Kim, J. S., & Bhuniya, S. (2019). Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells. Chemical Communications, 55(45), 6429-6432. https://doi.org/10.1039/c9cc01483g