TY - JOUR
T1 - Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells
AU - Bobba, Kondapa Naidu
AU - Binoy, Anupama
AU - Koo, Seyoung
AU - Nedungadi, Divya
AU - Podder, Arup
AU - Sharma, Amit
AU - Mishra, Nandita
AU - Kim, Jong Seung
AU - Bhuniya, Sankarprasad
N1 - Funding Information:
This work was supported by grants (DST-SERB, No. ECR/ 2015/00035, S. B.), (DST, SB/FT/LS-144/2012, N. M.) and (CRI, 2018R1A3B1052702, J. S. K.). A. B. is supported by a UGC-JRF/ SRF fellowship (F.2/2012(SA-I)) and D. N. is supported by an ICMR-JRF/SRF fellowship (20097).
Funding Information:
This work was supported by grants (DST-SERB, No. ECR/ 2015/00035, S. B.), (DST, SB/FT/LS-144/2012, N. M.) and (CRI, 2018R1A3B1052702, J. S. K.). A. B. is supported by a UGC-JRF/SRF fellowship (F.2/2012(SA-I)) and D. N. is supported by an ICMR-JRF/SRF fellowship (20097).
PY - 2019
Y1 - 2019
N2 - Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.
AB - Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.
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U2 - 10.1039/c9cc01483g
DO - 10.1039/c9cc01483g
M3 - Article
C2 - 31094377
AN - SCOPUS:85066451443
SN - 1359-7345
VL - 55
SP - 6429
EP - 6432
JO - Chemical Communications
JF - Chemical Communications
IS - 45
ER -
