Direct regulatory role of NKT cells in allogeneic graft survival is dependent on the quantitative strength of antigenicity

Keunhee Oh, Sanghee Kim, Se-Ho Park, Hua Gu, Derry Roopenian, Hyun Chung Doo, Su Kim Yon, Dong Sup Lee

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The role of NKT cells during immune responses is diverse, ranging from antiviral and antitumor activity to the regulation of autoimmune diseases; however, the regulatory function of CD1d-dependent NKT cells in rejection responses against allogeneic graft is uncertain. In this study, we demonstrated the direct regulatory effects of CD1d-dependent NKT cells using an allogeneic skin transplantation model. H-Y-mismatched skin graft survival was shortened in CD1d-/- recipients compared with wild-type recipients. Adoptive transfer of syngeneic NKT cells via splenocytes or hepatic mononuclear cells into CD1d-/- recipients restored graft survival times to those of wild-type recipients. α-Galactosylceramide, a specific activator of NKT cells, further prolonged graft survival. Although CD1d-dependent NKT cells did not extend skin graft survival in either major or complete minor histocompatibility-mismatched models, these cells affected graft survival in minor Ag mismatch models according to the magnitude of the antigenic difference. The afferent arm of NKT cell activation during transplantation required CD1d molecules expressed on host APCs and the migration of CD1d-dependent NKT cells into grafts. Moreover, the regulatory effects of CD1d-dependent NKT cells against alloantigen were primarily IL-10 dependent. Taken together, we concluded that CD1d-dependent NKT cells may directly affect the ontcome of allogeneic skin graft through an IL-10-dependent regulatory mechanism.

Original languageEnglish
Pages (from-to)2030-2036
Number of pages7
JournalJournal of Immunology
Volume174
Issue number4
Publication statusPublished - 2005 Feb 15

Fingerprint

Natural Killer T-Cells
Graft Survival
Transplants
Interleukin-10
Skin
CD1d Antigen
Galactosylceramides
Skin Transplantation
Histocompatibility
Isoantigens
Adoptive Transfer
Homologous Transplantation
Autoimmune Diseases
Antiviral Agents
Hepatocytes
Transplantation

ASJC Scopus subject areas

  • Immunology

Cite this

Direct regulatory role of NKT cells in allogeneic graft survival is dependent on the quantitative strength of antigenicity. / Oh, Keunhee; Kim, Sanghee; Park, Se-Ho; Gu, Hua; Roopenian, Derry; Doo, Hyun Chung; Yon, Su Kim; Lee, Dong Sup.

In: Journal of Immunology, Vol. 174, No. 4, 15.02.2005, p. 2030-2036.

Research output: Contribution to journalArticle

Oh, K, Kim, S, Park, S-H, Gu, H, Roopenian, D, Doo, HC, Yon, SK & Lee, DS 2005, 'Direct regulatory role of NKT cells in allogeneic graft survival is dependent on the quantitative strength of antigenicity', Journal of Immunology, vol. 174, no. 4, pp. 2030-2036.
Oh, Keunhee ; Kim, Sanghee ; Park, Se-Ho ; Gu, Hua ; Roopenian, Derry ; Doo, Hyun Chung ; Yon, Su Kim ; Lee, Dong Sup. / Direct regulatory role of NKT cells in allogeneic graft survival is dependent on the quantitative strength of antigenicity. In: Journal of Immunology. 2005 ; Vol. 174, No. 4. pp. 2030-2036.
@article{b5e8c2c2f39943cfb54967ba157bf9d8,
title = "Direct regulatory role of NKT cells in allogeneic graft survival is dependent on the quantitative strength of antigenicity",
abstract = "The role of NKT cells during immune responses is diverse, ranging from antiviral and antitumor activity to the regulation of autoimmune diseases; however, the regulatory function of CD1d-dependent NKT cells in rejection responses against allogeneic graft is uncertain. In this study, we demonstrated the direct regulatory effects of CD1d-dependent NKT cells using an allogeneic skin transplantation model. H-Y-mismatched skin graft survival was shortened in CD1d-/- recipients compared with wild-type recipients. Adoptive transfer of syngeneic NKT cells via splenocytes or hepatic mononuclear cells into CD1d-/- recipients restored graft survival times to those of wild-type recipients. α-Galactosylceramide, a specific activator of NKT cells, further prolonged graft survival. Although CD1d-dependent NKT cells did not extend skin graft survival in either major or complete minor histocompatibility-mismatched models, these cells affected graft survival in minor Ag mismatch models according to the magnitude of the antigenic difference. The afferent arm of NKT cell activation during transplantation required CD1d molecules expressed on host APCs and the migration of CD1d-dependent NKT cells into grafts. Moreover, the regulatory effects of CD1d-dependent NKT cells against alloantigen were primarily IL-10 dependent. Taken together, we concluded that CD1d-dependent NKT cells may directly affect the ontcome of allogeneic skin graft through an IL-10-dependent regulatory mechanism.",
author = "Keunhee Oh and Sanghee Kim and Se-Ho Park and Hua Gu and Derry Roopenian and Doo, {Hyun Chung} and Yon, {Su Kim} and Lee, {Dong Sup}",
year = "2005",
month = "2",
day = "15",
language = "English",
volume = "174",
pages = "2030--2036",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

TY - JOUR

T1 - Direct regulatory role of NKT cells in allogeneic graft survival is dependent on the quantitative strength of antigenicity

AU - Oh, Keunhee

AU - Kim, Sanghee

AU - Park, Se-Ho

AU - Gu, Hua

AU - Roopenian, Derry

AU - Doo, Hyun Chung

AU - Yon, Su Kim

AU - Lee, Dong Sup

PY - 2005/2/15

Y1 - 2005/2/15

N2 - The role of NKT cells during immune responses is diverse, ranging from antiviral and antitumor activity to the regulation of autoimmune diseases; however, the regulatory function of CD1d-dependent NKT cells in rejection responses against allogeneic graft is uncertain. In this study, we demonstrated the direct regulatory effects of CD1d-dependent NKT cells using an allogeneic skin transplantation model. H-Y-mismatched skin graft survival was shortened in CD1d-/- recipients compared with wild-type recipients. Adoptive transfer of syngeneic NKT cells via splenocytes or hepatic mononuclear cells into CD1d-/- recipients restored graft survival times to those of wild-type recipients. α-Galactosylceramide, a specific activator of NKT cells, further prolonged graft survival. Although CD1d-dependent NKT cells did not extend skin graft survival in either major or complete minor histocompatibility-mismatched models, these cells affected graft survival in minor Ag mismatch models according to the magnitude of the antigenic difference. The afferent arm of NKT cell activation during transplantation required CD1d molecules expressed on host APCs and the migration of CD1d-dependent NKT cells into grafts. Moreover, the regulatory effects of CD1d-dependent NKT cells against alloantigen were primarily IL-10 dependent. Taken together, we concluded that CD1d-dependent NKT cells may directly affect the ontcome of allogeneic skin graft through an IL-10-dependent regulatory mechanism.

AB - The role of NKT cells during immune responses is diverse, ranging from antiviral and antitumor activity to the regulation of autoimmune diseases; however, the regulatory function of CD1d-dependent NKT cells in rejection responses against allogeneic graft is uncertain. In this study, we demonstrated the direct regulatory effects of CD1d-dependent NKT cells using an allogeneic skin transplantation model. H-Y-mismatched skin graft survival was shortened in CD1d-/- recipients compared with wild-type recipients. Adoptive transfer of syngeneic NKT cells via splenocytes or hepatic mononuclear cells into CD1d-/- recipients restored graft survival times to those of wild-type recipients. α-Galactosylceramide, a specific activator of NKT cells, further prolonged graft survival. Although CD1d-dependent NKT cells did not extend skin graft survival in either major or complete minor histocompatibility-mismatched models, these cells affected graft survival in minor Ag mismatch models according to the magnitude of the antigenic difference. The afferent arm of NKT cell activation during transplantation required CD1d molecules expressed on host APCs and the migration of CD1d-dependent NKT cells into grafts. Moreover, the regulatory effects of CD1d-dependent NKT cells against alloantigen were primarily IL-10 dependent. Taken together, we concluded that CD1d-dependent NKT cells may directly affect the ontcome of allogeneic skin graft through an IL-10-dependent regulatory mechanism.

UR - http://www.scopus.com/inward/record.url?scp=13544262688&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13544262688&partnerID=8YFLogxK

M3 - Article

VL - 174

SP - 2030

EP - 2036

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -