Directional migration of mesenchymal stem cells under an SDF-1α gradient on a microfluidic device

Siwan Park, Hwanseok Jang, Byung Soo Kim, Changmo Hwang, Gi Seok Jeong, Yongdoo Park

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Homing of peripheral stem cells is regulated by one of the most representative homing factors, stromal cell-derived factor 1 alpha (SDF-1α), which specifically binds to the plasma membrane receptor CXCR4 of mesenchymal stem cells (MSCs) in order to initiate the signaling pathways that lead to directional migration and homing of stem cells. This complex homing process and directional migration of stem cells have been mimicked on a microfluidic device that is capable of generating a chemokine gradient within the collagen matrix and embedding endothelial cell (EC) monolayers to mimic blood vessels. On the microfluidic device, stem cells showed directional migration toward the higher concentration of SDF-1α, whereas treatment with the CXCR4 antagonist AMD3100 caused loss of directionality of stem cells. Furthermore, inhibition of stem cell’s main migratory signaling pathways, Rho-ROCK and Rac pathways, caused blockage of actomyosin and lamellipodia formation, decreasing the migration distance but maintaining directionality. Stem cell homing regulated by SDF-1α caused directional migration of stem cells, while the migratory ability was affected by the activation of migration-related signaling pathways.

Original languageEnglish
Article numbere0184595
JournalPLoS One
Volume12
Issue number9
DOIs
Publication statusPublished - 2017 Sep 1

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Lab-On-A-Chip Devices
Chemokine CXCL12
Stem cells
Mesenchymal Stromal Cells
Microfluidics
stem cells
Stem Cells
CXCR4 Receptors
Actomyosin
Pseudopodia
pseudopodia
chemokine CXCL12
Chemokines
Blood Vessels
Endothelial cells
Blood vessels
chemokines
Cell membranes
Collagen
Endothelial Cells

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Directional migration of mesenchymal stem cells under an SDF-1α gradient on a microfluidic device. / Park, Siwan; Jang, Hwanseok; Kim, Byung Soo; Hwang, Changmo; Jeong, Gi Seok; Park, Yongdoo.

In: PLoS One, Vol. 12, No. 9, e0184595, 01.09.2017.

Research output: Contribution to journalArticle

Park, Siwan ; Jang, Hwanseok ; Kim, Byung Soo ; Hwang, Changmo ; Jeong, Gi Seok ; Park, Yongdoo. / Directional migration of mesenchymal stem cells under an SDF-1α gradient on a microfluidic device. In: PLoS One. 2017 ; Vol. 12, No. 9.
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