Disaggregating shear stress: The roles of cell deformability and fibrinogen concentration

Shubin Xue, Byoung Kwon Lee, Sehyun Shin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Red blood cell (RBC) aggregation is greatly affected by cell deformability and reduced deformability and increased RBC aggregation are frequently observed in hypertension, diabetes mellitus, and sepsis, thus measurement of both these parameters is essential. In this study, we investigated the effects of cell deformability and fibrinogen concentration on disaggregating shear stress (DSS). The DSS was measured with varying cell deformability and geometry. The deformability of cells was gradually decreased with increasing concentration of glutaraldehyde (0.001~0.005%) or heat treatment at 49.0°C for increasing time intervals (0~7 min), which resulted in a progressive increase in the DSS. However, RBC rigidification by either glutaraldehyde or heat treatment did not cause the same effect on RBC aggregation as deformability did. The effect of cell deformability on DSS was significantly increased with an increase in fibrinogen concentration (2~6 g/L). These results imply that reduced cell deformability and increased fibrinogen levels play a synergistic role in increasing DSS, which could be used as a novel independent hemorheological index to characterize microcirculatory diseases, such as diabetic complications with high sensitivity.

Original languageEnglish
Pages (from-to)231-240
Number of pages10
JournalClinical Hemorheology and Microcirculation
Volume55
Issue number2
DOIs
Publication statusPublished - 2013 Dec 12

Fingerprint

Fibrinogen
Cell Aggregation
Erythrocytes
Glutaral
Hot Temperature
Diabetes Complications
Sepsis
Diabetes Mellitus
Hypertension

Keywords

  • Aggregation
  • Deformability
  • Disaggregating shear stress
  • Fibrinogen
  • RBC

ASJC Scopus subject areas

  • Hematology
  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Disaggregating shear stress : The roles of cell deformability and fibrinogen concentration. / Xue, Shubin; Lee, Byoung Kwon; Shin, Sehyun.

In: Clinical Hemorheology and Microcirculation, Vol. 55, No. 2, 12.12.2013, p. 231-240.

Research output: Contribution to journalArticle

@article{bf8bc54a420d4582825edf10bab956dd,
title = "Disaggregating shear stress: The roles of cell deformability and fibrinogen concentration",
abstract = "Red blood cell (RBC) aggregation is greatly affected by cell deformability and reduced deformability and increased RBC aggregation are frequently observed in hypertension, diabetes mellitus, and sepsis, thus measurement of both these parameters is essential. In this study, we investigated the effects of cell deformability and fibrinogen concentration on disaggregating shear stress (DSS). The DSS was measured with varying cell deformability and geometry. The deformability of cells was gradually decreased with increasing concentration of glutaraldehyde (0.001~0.005{\%}) or heat treatment at 49.0°C for increasing time intervals (0~7 min), which resulted in a progressive increase in the DSS. However, RBC rigidification by either glutaraldehyde or heat treatment did not cause the same effect on RBC aggregation as deformability did. The effect of cell deformability on DSS was significantly increased with an increase in fibrinogen concentration (2~6 g/L). These results imply that reduced cell deformability and increased fibrinogen levels play a synergistic role in increasing DSS, which could be used as a novel independent hemorheological index to characterize microcirculatory diseases, such as diabetic complications with high sensitivity.",
keywords = "Aggregation, Deformability, Disaggregating shear stress, Fibrinogen, RBC",
author = "Shubin Xue and Lee, {Byoung Kwon} and Sehyun Shin",
year = "2013",
month = "12",
day = "12",
doi = "10.3233/CH-2012-1627",
language = "English",
volume = "55",
pages = "231--240",
journal = "Clinical Hemorheology and Microcirculation",
issn = "1386-0291",
publisher = "IOS Press",
number = "2",

}

TY - JOUR

T1 - Disaggregating shear stress

T2 - The roles of cell deformability and fibrinogen concentration

AU - Xue, Shubin

AU - Lee, Byoung Kwon

AU - Shin, Sehyun

PY - 2013/12/12

Y1 - 2013/12/12

N2 - Red blood cell (RBC) aggregation is greatly affected by cell deformability and reduced deformability and increased RBC aggregation are frequently observed in hypertension, diabetes mellitus, and sepsis, thus measurement of both these parameters is essential. In this study, we investigated the effects of cell deformability and fibrinogen concentration on disaggregating shear stress (DSS). The DSS was measured with varying cell deformability and geometry. The deformability of cells was gradually decreased with increasing concentration of glutaraldehyde (0.001~0.005%) or heat treatment at 49.0°C for increasing time intervals (0~7 min), which resulted in a progressive increase in the DSS. However, RBC rigidification by either glutaraldehyde or heat treatment did not cause the same effect on RBC aggregation as deformability did. The effect of cell deformability on DSS was significantly increased with an increase in fibrinogen concentration (2~6 g/L). These results imply that reduced cell deformability and increased fibrinogen levels play a synergistic role in increasing DSS, which could be used as a novel independent hemorheological index to characterize microcirculatory diseases, such as diabetic complications with high sensitivity.

AB - Red blood cell (RBC) aggregation is greatly affected by cell deformability and reduced deformability and increased RBC aggregation are frequently observed in hypertension, diabetes mellitus, and sepsis, thus measurement of both these parameters is essential. In this study, we investigated the effects of cell deformability and fibrinogen concentration on disaggregating shear stress (DSS). The DSS was measured with varying cell deformability and geometry. The deformability of cells was gradually decreased with increasing concentration of glutaraldehyde (0.001~0.005%) or heat treatment at 49.0°C for increasing time intervals (0~7 min), which resulted in a progressive increase in the DSS. However, RBC rigidification by either glutaraldehyde or heat treatment did not cause the same effect on RBC aggregation as deformability did. The effect of cell deformability on DSS was significantly increased with an increase in fibrinogen concentration (2~6 g/L). These results imply that reduced cell deformability and increased fibrinogen levels play a synergistic role in increasing DSS, which could be used as a novel independent hemorheological index to characterize microcirculatory diseases, such as diabetic complications with high sensitivity.

KW - Aggregation

KW - Deformability

KW - Disaggregating shear stress

KW - Fibrinogen

KW - RBC

UR - http://www.scopus.com/inward/record.url?scp=84889688160&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84889688160&partnerID=8YFLogxK

U2 - 10.3233/CH-2012-1627

DO - 10.3233/CH-2012-1627

M3 - Article

C2 - 23089881

AN - SCOPUS:84889688160

VL - 55

SP - 231

EP - 240

JO - Clinical Hemorheology and Microcirculation

JF - Clinical Hemorheology and Microcirculation

SN - 1386-0291

IS - 2

ER -