Disassembly of focal adhesions during apoptosis of endothelial cell line ECV304 infected with Orientia tsutsugamushi

Sun Ho Kee; Kyung A. Cho; Mee Kyung Kim; Byung Uk Lim; Woo Hyun Chang; Jae Seung Kang

doi:10.1006/mpat.1999.0304

Disassembly of focal adhesions during apoptosis of endothelial cell line ECV304 infected with Orientia tsutsugamushi

Sun Ho Kee, Kyung A. Cho, Mee Kyung Kim, Byung Uk Lim, Woo Hyun Chang, Jae Seung Kang

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Many bacterial pathogens induce apoptosis in their host cells. We observed the cellular effect of ECV304 cells infected with Orientia tsutsugamushi. The infected cells became rounded and floated in culture supernatant. These floating cells as well as adherent cells exhibited typical features of apoptosis, such as DNA fragmentation and TUNEL staining. As many cells detached from growth substrate, we examined the focal adhesion using the immunofluorescence assay method and observed decreased focal adhesions in heavily infected cells. As endothelial cells could undergo apoptosis by the loss of focal adhesions, this change of focal adhesions may account for the Orientia-induced apoptosis.

Original language	English
Pages (from-to)	265-271
Number of pages	7
Journal	Microbial Pathogenesis
Volume	27
Issue number	5
DOIs	https://doi.org/10.1006/mpat.1999.0304
Publication status	Published - 1999 Nov

Keywords

Apoptosis
Focal adhesions
Orientia tsutsugamushi

ASJC Scopus subject areas

Microbiology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1006/mpat.1999.0304

Cite this

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title = "Disassembly of focal adhesions during apoptosis of endothelial cell line ECV304 infected with Orientia tsutsugamushi",

abstract = "Many bacterial pathogens induce apoptosis in their host cells. We observed the cellular effect of ECV304 cells infected with Orientia tsutsugamushi. The infected cells became rounded and floated in culture supernatant. These floating cells as well as adherent cells exhibited typical features of apoptosis, such as DNA fragmentation and TUNEL staining. As many cells detached from growth substrate, we examined the focal adhesion using the immunofluorescence assay method and observed decreased focal adhesions in heavily infected cells. As endothelial cells could undergo apoptosis by the loss of focal adhesions, this change of focal adhesions may account for the Orientia-induced apoptosis.",

keywords = "Apoptosis, Focal adhesions, Orientia tsutsugamushi",

author = "Kee, {Sun Ho} and Cho, {Kyung A.} and Kim, {Mee Kyung} and Lim, {Byung Uk} and Chang, {Woo Hyun} and Kang, {Jae Seung}",

note = "Funding Information: This study was supported by a Basic Medical Research Fund from the Ministry of Education of the Republic of Korea, 1997.",

year = "1999",

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doi = "10.1006/mpat.1999.0304",

language = "English",

volume = "27",

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T1 - Disassembly of focal adhesions during apoptosis of endothelial cell line ECV304 infected with Orientia tsutsugamushi

AU - Kee, Sun Ho

AU - Cho, Kyung A.

AU - Kim, Mee Kyung

AU - Lim, Byung Uk

AU - Chang, Woo Hyun

AU - Kang, Jae Seung

N1 - Funding Information: This study was supported by a Basic Medical Research Fund from the Ministry of Education of the Republic of Korea, 1997.

PY - 1999/11

Y1 - 1999/11

N2 - Many bacterial pathogens induce apoptosis in their host cells. We observed the cellular effect of ECV304 cells infected with Orientia tsutsugamushi. The infected cells became rounded and floated in culture supernatant. These floating cells as well as adherent cells exhibited typical features of apoptosis, such as DNA fragmentation and TUNEL staining. As many cells detached from growth substrate, we examined the focal adhesion using the immunofluorescence assay method and observed decreased focal adhesions in heavily infected cells. As endothelial cells could undergo apoptosis by the loss of focal adhesions, this change of focal adhesions may account for the Orientia-induced apoptosis.

AB - Many bacterial pathogens induce apoptosis in their host cells. We observed the cellular effect of ECV304 cells infected with Orientia tsutsugamushi. The infected cells became rounded and floated in culture supernatant. These floating cells as well as adherent cells exhibited typical features of apoptosis, such as DNA fragmentation and TUNEL staining. As many cells detached from growth substrate, we examined the focal adhesion using the immunofluorescence assay method and observed decreased focal adhesions in heavily infected cells. As endothelial cells could undergo apoptosis by the loss of focal adhesions, this change of focal adhesions may account for the Orientia-induced apoptosis.

KW - Apoptosis

KW - Focal adhesions

KW - Orientia tsutsugamushi

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U2 - 10.1006/mpat.1999.0304

DO - 10.1006/mpat.1999.0304

M3 - Article

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AN - SCOPUS:0032750460

VL - 27

SP - 265

EP - 271

JO - Microbial Pathogenesis

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ER -

Disassembly of focal adhesions during apoptosis of endothelial cell line ECV304 infected with Orientia tsutsugamushi

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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