Disassembly of focal adhesions during apoptosis of endothelial cell line ECV304 infected with Orientia tsutsugamushi

Sun Ho Kee, Kyung A. Cho, Mee Kyung Kim, Byung Uk Lim, Woo Hyun Chang, Jae Seung Kang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Many bacterial pathogens induce apoptosis in their host cells. We observed the cellular effect of ECV304 cells infected with Orientia tsutsugamushi. The infected cells became rounded and floated in culture supernatant. These floating cells as well as adherent cells exhibited typical features of apoptosis, such as DNA fragmentation and TUNEL staining. As many cells detached from growth substrate, we examined the focal adhesion using the immunofluorescence assay method and observed decreased focal adhesions in heavily infected cells. As endothelial cells could undergo apoptosis by the loss of focal adhesions, this change of focal adhesions may account for the Orientia-induced apoptosis.

Original languageEnglish
Pages (from-to)265-271
Number of pages7
JournalMicrobial Pathogenesis
Volume27
Issue number5
DOIs
Publication statusPublished - 1999 Nov

Keywords

  • Apoptosis
  • Focal adhesions
  • Orientia tsutsugamushi

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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