Abstract
Many bacterial pathogens induce apoptosis in their host cells. We observed the cellular effect of ECV304 cells infected with Orientia tsutsugamushi. The infected cells became rounded and floated in culture supernatant. These floating cells as well as adherent cells exhibited typical features of apoptosis, such as DNA fragmentation and TUNEL staining. As many cells detached from growth substrate, we examined the focal adhesion using the immunofluorescence assay method and observed decreased focal adhesions in heavily infected cells. As endothelial cells could undergo apoptosis by the loss of focal adhesions, this change of focal adhesions may account for the Orientia-induced apoptosis.
| Original language | English |
|---|---|
| Pages (from-to) | 265-271 |
| Number of pages | 7 |
| Journal | Microbial Pathogenesis |
| Volume | 27 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1999 Nov 1 |
| Externally published | Yes |
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Keywords
- Apoptosis
- Focal adhesions
- Orientia tsutsugamushi
ASJC Scopus subject areas
- Microbiology
- Infectious Diseases
Cite this
Disassembly of focal adhesions during apoptosis of endothelial cell line ECV304 infected with Orientia tsutsugamushi. / Kee, Sun-Ho; Cho, Kyung A.; Kim, Mee Kyung; Lim, Byung Uk; Chang, Woo Hyun; Kang, Jae Seung.
In: Microbial Pathogenesis, Vol. 27, No. 5, 01.11.1999, p. 265-271.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Disassembly of focal adhesions during apoptosis of endothelial cell line ECV304 infected with Orientia tsutsugamushi
AU - Kee, Sun-Ho
AU - Cho, Kyung A.
AU - Kim, Mee Kyung
AU - Lim, Byung Uk
AU - Chang, Woo Hyun
AU - Kang, Jae Seung
PY - 1999/11/1
Y1 - 1999/11/1
N2 - Many bacterial pathogens induce apoptosis in their host cells. We observed the cellular effect of ECV304 cells infected with Orientia tsutsugamushi. The infected cells became rounded and floated in culture supernatant. These floating cells as well as adherent cells exhibited typical features of apoptosis, such as DNA fragmentation and TUNEL staining. As many cells detached from growth substrate, we examined the focal adhesion using the immunofluorescence assay method and observed decreased focal adhesions in heavily infected cells. As endothelial cells could undergo apoptosis by the loss of focal adhesions, this change of focal adhesions may account for the Orientia-induced apoptosis.
AB - Many bacterial pathogens induce apoptosis in their host cells. We observed the cellular effect of ECV304 cells infected with Orientia tsutsugamushi. The infected cells became rounded and floated in culture supernatant. These floating cells as well as adherent cells exhibited typical features of apoptosis, such as DNA fragmentation and TUNEL staining. As many cells detached from growth substrate, we examined the focal adhesion using the immunofluorescence assay method and observed decreased focal adhesions in heavily infected cells. As endothelial cells could undergo apoptosis by the loss of focal adhesions, this change of focal adhesions may account for the Orientia-induced apoptosis.
KW - Apoptosis
KW - Focal adhesions
KW - Orientia tsutsugamushi
UR - http://www.scopus.com/inward/record.url?scp=0032750460&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032750460&partnerID=8YFLogxK
U2 - 10.1006/mpat.1999.0304
DO - 10.1006/mpat.1999.0304
M3 - Article
C2 - 10545254
AN - SCOPUS:0032750460
VL - 27
SP - 265
EP - 271
JO - Microbial Pathogenesis
JF - Microbial Pathogenesis
SN - 0882-4010
IS - 5
ER -