Discovery of candidate genes and pathways regulating oviduct development in chickens

Gwonhwa Song, Hee Won Seo, Jin Won Choi, Deivendran Rengaraj, Tae Min Kim, Bo Ram Lee, Young Min Kim, Tae Won Yun, Jae Wook Jeong, Jae Yong Han

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Estrogen is a key regulator in the development of the female reproductive system. It also stimulates oviduct development in immature chicks. We identified candidate genes and pathways associated with the development of chicken oviducts. A pellet containing the synthetic estrogen analog diethylstilbestrol (DES) was implanted subcutaneously in 1-wk-old female chicks for 10 days. The pellet was removed from half the group for 10 days, and an additional dose was given for a further 10 days. Total RNA was extracted from the oviducts of DES-treated and untreated chicks and subjected to an Affymetrix chicken GeneChip analysis. We found differential expression of 2290 and 1745 transcripts from the oviducts that were treated with DES once and twice, respectively. We also found a twofold or greater change in the expression of 77 and 390 transcripts between the two control and DES-treated time points, respectively, while we found a change in the expression of 10 transcripts that were common to all groups. Analyses of real-time PCR and in situ hybridization of selected genes confirmed the validity of the gene expression patterns observed in the microarray analysis. In particular, CCRN4L, FAM26F, HAS2, NELF, and NTM were upregulated in the DES-treated chicken oviducts. High-throughput analysis revealed that the differentially expressed genes were related to tubular formation, epithelial differentiation, hormone interactions, nerve development, and tissue remodeling in the chicken oviduct. This study provides novel insights into candidate genes regulating oviduct development and differentiation via estrogen. The identified genes may serve as biomarkers of reproductive tract development in chicks.

Original languageEnglish
Pages (from-to)306-314
Number of pages9
JournalBiology of Reproduction
Volume85
Issue number2
DOIs
Publication statusPublished - 2011 Aug 1
Externally publishedYes

Fingerprint

Oviducts
Genetic Association Studies
Diethylstilbestrol
Chickens
Estradiol Congeners
Genes
Estrogens
Nerve Tissue
Microarray Analysis
In Situ Hybridization
Real-Time Polymerase Chain Reaction
Biomarkers
Hormones
RNA
Gene Expression

Keywords

  • Chicken
  • Diethylstilbestrol
  • Estradiol/estradiol receptor
  • Estrogen
  • Female reproductive tract
  • Microarray analysis
  • Oviduct
  • Uterus

ASJC Scopus subject areas

  • Cell Biology

Cite this

Song, G., Seo, H. W., Choi, J. W., Rengaraj, D., Kim, T. M., Lee, B. R., ... Han, J. Y. (2011). Discovery of candidate genes and pathways regulating oviduct development in chickens. Biology of Reproduction, 85(2), 306-314. https://doi.org/10.1095/biolreprod.110.089227

Discovery of candidate genes and pathways regulating oviduct development in chickens. / Song, Gwonhwa; Seo, Hee Won; Choi, Jin Won; Rengaraj, Deivendran; Kim, Tae Min; Lee, Bo Ram; Kim, Young Min; Yun, Tae Won; Jeong, Jae Wook; Han, Jae Yong.

In: Biology of Reproduction, Vol. 85, No. 2, 01.08.2011, p. 306-314.

Research output: Contribution to journalArticle

Song, G, Seo, HW, Choi, JW, Rengaraj, D, Kim, TM, Lee, BR, Kim, YM, Yun, TW, Jeong, JW & Han, JY 2011, 'Discovery of candidate genes and pathways regulating oviduct development in chickens', Biology of Reproduction, vol. 85, no. 2, pp. 306-314. https://doi.org/10.1095/biolreprod.110.089227
Song, Gwonhwa ; Seo, Hee Won ; Choi, Jin Won ; Rengaraj, Deivendran ; Kim, Tae Min ; Lee, Bo Ram ; Kim, Young Min ; Yun, Tae Won ; Jeong, Jae Wook ; Han, Jae Yong. / Discovery of candidate genes and pathways regulating oviduct development in chickens. In: Biology of Reproduction. 2011 ; Vol. 85, No. 2. pp. 306-314.
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