Disposable amperometric biosensor based on nanostructured bacteriophages for glucose detection

Yu Ri Kang, Kyung Hoon Hwang, Ju Hwan Kim, Chang Hoon Nam, Soo Won Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The selection of electrode material profoundly influences biosensor science and engineering, as it heavily influences biosensor sensitivity. Here we propose a novel electrochemical detection method using a working electrode consisting of bio-nanowires from genetically modified filamentous phages and nanoparticles. fd-tet p8MMM filamentous phages displaying a three-methionine (MMM) peptide on the major coat protein pVIII (designated p8MMM phages) were immobilized on the active area of an electrochemical sensor through physical adsorption and chemical bonding. Bio-nanowires composed of p8MMM phages and silver nanoparticles facilitated sensitive, rapid and selective detection of particular molecules. We explored whether the composite electrode with bio-nanowires was an effective platform to detect the glucose oxidase. The current response of the bio-nanowire sensor was high at various glucose concentrations (0.1 μM-0.1 mM). This method provides a considerable advantage to demonstrate analyte detection over low concentration ranges. Especially, phage-enabled bio-nanowires can serve as receptors with high affinity and specificity for the detection of particular biomolecules and provide a convenient platform for designing site-directed multifunctional scaffolds based on bacteriophages and may serve as a simple method for label-free detection.

Original languageEnglish
Article number105804
JournalMeasurement Science and Technology
Volume21
Issue number10
DOIs
Publication statusPublished - 2010 Oct

Keywords

  • Bacteriophage
  • Biosensor application
  • Disposable analytical system
  • Label-free detection

ASJC Scopus subject areas

  • Instrumentation
  • Engineering (miscellaneous)
  • Applied Mathematics

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