Abstract
The laser-diffraction technique has been applied to design a microfluidic channel for measuring red blood cell deformability over a range of shear stress. A laser beam traverses a diluted blood suspension and is diffracted by RBCs in the volume. The diffraction patterns are captured by a CCD-video camera, linked to a frame grabber integrated with a computer. When deforming under decreasing shear stress in the microchannel, RBCs change gradually from the prolate ellipsoid towards a circular biconcave morphology. Both the laser-diffraction image and pressure were measured with respect to time, which enable to determine the elongation index (EI) and the shear stress. The range of shear stress is 0-20Pa and the measuring time is less than 2min. The elongation index (EI) is determined from an isointensity curve in the diffraction pattern using an ellipse-fitting program. The key advantage of this design is the incorporation of a disposable element that holds the blood sample, which enables the present system to be easily used in a clinical setting.
| Original language | English |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Editors | D.V. Nicolau |
| Pages | 68-77 |
| Number of pages | 10 |
| Volume | 5651 |
| DOIs | |
| Publication status | Published - 2005 |
| Externally published | Yes |
| Event | Biomedical Applications of Micro- and Nanoengineering II - Sydney, NSW, Australia Duration: 2004 Dec 13 → 2004 Dec 15 |
Other
| Other | Biomedical Applications of Micro- and Nanoengineering II |
|---|---|
| Country | Australia |
| City | Sydney, NSW |
| Period | 04/12/13 → 04/12/15 |
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Keywords
- Deformability
- Diffraction
- Disposable
- RBC
- Shear stress
ASJC Scopus subject areas
- Engineering(all)
Cite this
Disposable biosensor for measuring red blood cell deformability using laser-diffraction technique. / Shin, Sehyun; Ku, Yunhee; Park, Myungsu; Suh, Jangsoo.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. ed. / D.V. Nicolau. Vol. 5651 2005. p. 68-77 12.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Disposable biosensor for measuring red blood cell deformability using laser-diffraction technique
AU - Shin, Sehyun
AU - Ku, Yunhee
AU - Park, Myungsu
AU - Suh, Jangsoo
PY - 2005
Y1 - 2005
N2 - The laser-diffraction technique has been applied to design a microfluidic channel for measuring red blood cell deformability over a range of shear stress. A laser beam traverses a diluted blood suspension and is diffracted by RBCs in the volume. The diffraction patterns are captured by a CCD-video camera, linked to a frame grabber integrated with a computer. When deforming under decreasing shear stress in the microchannel, RBCs change gradually from the prolate ellipsoid towards a circular biconcave morphology. Both the laser-diffraction image and pressure were measured with respect to time, which enable to determine the elongation index (EI) and the shear stress. The range of shear stress is 0-20Pa and the measuring time is less than 2min. The elongation index (EI) is determined from an isointensity curve in the diffraction pattern using an ellipse-fitting program. The key advantage of this design is the incorporation of a disposable element that holds the blood sample, which enables the present system to be easily used in a clinical setting.
AB - The laser-diffraction technique has been applied to design a microfluidic channel for measuring red blood cell deformability over a range of shear stress. A laser beam traverses a diluted blood suspension and is diffracted by RBCs in the volume. The diffraction patterns are captured by a CCD-video camera, linked to a frame grabber integrated with a computer. When deforming under decreasing shear stress in the microchannel, RBCs change gradually from the prolate ellipsoid towards a circular biconcave morphology. Both the laser-diffraction image and pressure were measured with respect to time, which enable to determine the elongation index (EI) and the shear stress. The range of shear stress is 0-20Pa and the measuring time is less than 2min. The elongation index (EI) is determined from an isointensity curve in the diffraction pattern using an ellipse-fitting program. The key advantage of this design is the incorporation of a disposable element that holds the blood sample, which enables the present system to be easily used in a clinical setting.
KW - Deformability
KW - Diffraction
KW - Disposable
KW - RBC
KW - Shear stress
UR - http://www.scopus.com/inward/record.url?scp=21844460795&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21844460795&partnerID=8YFLogxK
U2 - 10.1117/12.581265
DO - 10.1117/12.581265
M3 - Conference contribution
AN - SCOPUS:21844460795
VL - 5651
SP - 68
EP - 77
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
A2 - Nicolau, D.V.
ER -