Disposable biosensor for measuring red blood cell deformability using laser-diffraction technique

Sehyun Shin, Yunhee Ku, Myungsu Park, Jangsoo Suh

Research output: Contribution to journalConference articlepeer-review

1 Citation (Scopus)

Abstract

The laser-diffraction technique has been applied to design a microfluidic channel for measuring red blood cell deformability over a range of shear stress. A laser beam traverses a diluted blood suspension and is diffracted by RBCs in the volume. The diffraction patterns are captured by a CCD-video camera, linked to a frame grabber integrated with a computer. When deforming under decreasing shear stress in the microchannel, RBCs change gradually from the prolate ellipsoid towards a circular biconcave morphology. Both the laser-diffraction image and pressure were measured with respect to time, which enable to determine the elongation index (EI) and the shear stress. The range of shear stress is 0-20Pa and the measuring time is less than 2min. The elongation index (EI) is determined from an isointensity curve in the diffraction pattern using an ellipse-fitting program. The key advantage of this design is the incorporation of a disposable element that holds the blood sample, which enables the present system to be easily used in a clinical setting.

Original languageEnglish
Article number12
Pages (from-to)68-77
Number of pages10
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5651
DOIs
Publication statusPublished - 2005
Externally publishedYes
EventBiomedical Applications of Micro- and Nanoengineering II - Sydney, NSW, Australia
Duration: 2004 Dec 132004 Dec 15

Keywords

  • Deformability
  • Diffraction
  • Disposable
  • RBC
  • Shear stress

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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