Disposable biosensor for measuring red blood cell deformability using laser-diffraction technique

Sehyun Shin, Yunhee Ku, Myungsu Park, Jangsoo Suh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The laser-diffraction technique has been applied to design a microfluidic channel for measuring red blood cell deformability over a range of shear stress. A laser beam traverses a diluted blood suspension and is diffracted by RBCs in the volume. The diffraction patterns are captured by a CCD-video camera, linked to a frame grabber integrated with a computer. When deforming under decreasing shear stress in the microchannel, RBCs change gradually from the prolate ellipsoid towards a circular biconcave morphology. Both the laser-diffraction image and pressure were measured with respect to time, which enable to determine the elongation index (EI) and the shear stress. The range of shear stress is 0-20Pa and the measuring time is less than 2min. The elongation index (EI) is determined from an isointensity curve in the diffraction pattern using an ellipse-fitting program. The key advantage of this design is the incorporation of a disposable element that holds the blood sample, which enables the present system to be easily used in a clinical setting.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsD.V. Nicolau
Pages68-77
Number of pages10
Volume5651
DOIs
Publication statusPublished - 2005
Externally publishedYes
EventBiomedical Applications of Micro- and Nanoengineering II - Sydney, NSW, Australia
Duration: 2004 Dec 132004 Dec 15

Other

OtherBiomedical Applications of Micro- and Nanoengineering II
CountryAustralia
CitySydney, NSW
Period04/12/1304/12/15

Keywords

  • Deformability
  • Diffraction
  • Disposable
  • RBC
  • Shear stress

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Shin, S., Ku, Y., Park, M., & Suh, J. (2005). Disposable biosensor for measuring red blood cell deformability using laser-diffraction technique. In D. V. Nicolau (Ed.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 5651, pp. 68-77). [12] https://doi.org/10.1117/12.581265