Abstract
The laser-diffraction technique has been applied to design a microfluidic channel for measuring red blood cell deformability over a range of shear stress. A laser beam traverses a diluted blood suspension and is diffracted by RBCs in the volume. The diffraction patterns are captured by a CCD-video camera, linked to a frame grabber integrated with a computer. When deforming under decreasing shear stress in the microchannel, RBCs change gradually from the prolate ellipsoid towards a circular biconcave morphology. Both the laser-diffraction image and pressure were measured with respect to time, which enable to determine the elongation index (EI) and the shear stress. The range of shear stress is 0-20Pa and the measuring time is less than 2min. The elongation index (EI) is determined from an isointensity curve in the diffraction pattern using an ellipse-fitting program. The key advantage of this design is the incorporation of a disposable element that holds the blood sample, which enables the present system to be easily used in a clinical setting.
Original language | English |
---|---|
Article number | 12 |
Pages (from-to) | 68-77 |
Number of pages | 10 |
Journal | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
Volume | 5651 |
DOIs | |
Publication status | Published - 2005 |
Externally published | Yes |
Event | Biomedical Applications of Micro- and Nanoengineering II - Sydney, NSW, Australia Duration: 2004 Dec 13 → 2004 Dec 15 |
Keywords
- Deformability
- Diffraction
- Disposable
- RBC
- Shear stress
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging