Disposable laser-diffraction ektacytometry to measure red blood cell deformability

Sehyun Shin, Yunhee Ku, Myungsu Park, Lijuan Zhang, Joohee Jang, Jangsoo Suh

Research output: Contribution to journalConference article

Abstract

The laser-diffraction technique has been applied to design a slit rheometer for measuring red blood cell deformability over a range of shear stress. A laser beam traverses a diluted blood suspension and is diffracted by RBCs in the volume. The diffraction patterns are captured by a CCD-video camera, linked to a frame grabber integrated with a computer. When deforming under decreasing shear stress, RBCs change gradually from the prolate ellipsoid towards a circular biconcave morphology. Both the laser-diffraction image and pressure were measured with respect to time. Which enable to determine the elongation index (El) and the shear stress. The range of shear stress is 0-35 Pa and the measuring time is less than 2 min. The elongation index(EI) is determined from an isointensity curve in the diffraction pattern using an ellipse-fitting program. The key advantage of this design is the incorporation of a disposable element that holds the blood sample, which enables the present system to be easily used in a clinical setting.

Original languageEnglish
Article number30
Pages (from-to)168-176
Number of pages9
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5630 I
DOIs
Publication statusPublished - 2005
Externally publishedYes
EventOptics in Health Care and Biomedical Optics: Diagnostics and Treatment II - Beijing, China
Duration: 2004 Nov 82004 Nov 12

Keywords

  • Deformability
  • Diffraction
  • Disposable
  • RBC
  • Shear stress

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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