Disposable laser-diffraction ektacytometry to measure red blood cell deformability

Sehyun Shin, Yunhee Ku, Myungsu Park, Lijuan Zhang, Joohee Jang, Jangsoo Suh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The laser-diffraction technique has been applied to design a slit rheometer for measuring red blood cell deformability over a range of shear stress. A laser beam traverses a diluted blood suspension and is diffracted by RBCs in the volume. The diffraction patterns are captured by a CCD-video camera, linked to a frame grabber integrated with a computer. When deforming under decreasing shear stress, RBCs change gradually from the prolate ellipsoid towards a circular biconcave morphology. Both the laser-diffraction image and pressure were measured with respect to time. Which enable to determine the elongation index (El) and the shear stress. The range of shear stress is 0-35 Pa and the measuring time is less than 2 min. The elongation index(EI) is determined from an isointensity curve in the diffraction pattern using an ellipse-fitting program. The key advantage of this design is the incorporation of a disposable element that holds the blood sample, which enables the present system to be easily used in a clinical setting.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsB. Chance, M. Chen, A.E.T. Chiou, Q. Luo
Pages168-176
Number of pages9
Volume5630 I
DOIs
Publication statusPublished - 2005
Externally publishedYes
EventOptics in Health Care and Biomedical Optics: Diagnostics and Treatment II - Beijing, China
Duration: 2004 Nov 82004 Nov 12

Other

OtherOptics in Health Care and Biomedical Optics: Diagnostics and Treatment II
CountryChina
CityBeijing
Period04/11/804/11/12

Fingerprint

Formability
Shear stress
Blood
Diffraction
Cells
Lasers
Diffraction patterns
Elongation
Rheometers
Video cameras
CCD cameras
Laser beams

Keywords

  • Deformability
  • Diffraction
  • Disposable
  • RBC
  • Shear stress

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Shin, S., Ku, Y., Park, M., Zhang, L., Jang, J., & Suh, J. (2005). Disposable laser-diffraction ektacytometry to measure red blood cell deformability. In B. Chance, M. Chen, A. E. T. Chiou, & Q. Luo (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 5630 I, pp. 168-176). [30] https://doi.org/10.1117/12.575957

Disposable laser-diffraction ektacytometry to measure red blood cell deformability. / Shin, Sehyun; Ku, Yunhee; Park, Myungsu; Zhang, Lijuan; Jang, Joohee; Suh, Jangsoo.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. ed. / B. Chance; M. Chen; A.E.T. Chiou; Q. Luo. Vol. 5630 I 2005. p. 168-176 30.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shin, S, Ku, Y, Park, M, Zhang, L, Jang, J & Suh, J 2005, Disposable laser-diffraction ektacytometry to measure red blood cell deformability. in B Chance, M Chen, AET Chiou & Q Luo (eds), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 5630 I, 30, pp. 168-176, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, Beijing, China, 04/11/8. https://doi.org/10.1117/12.575957
Shin S, Ku Y, Park M, Zhang L, Jang J, Suh J. Disposable laser-diffraction ektacytometry to measure red blood cell deformability. In Chance B, Chen M, Chiou AET, Luo Q, editors, Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 5630 I. 2005. p. 168-176. 30 https://doi.org/10.1117/12.575957
Shin, Sehyun ; Ku, Yunhee ; Park, Myungsu ; Zhang, Lijuan ; Jang, Joohee ; Suh, Jangsoo. / Disposable laser-diffraction ektacytometry to measure red blood cell deformability. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. editor / B. Chance ; M. Chen ; A.E.T. Chiou ; Q. Luo. Vol. 5630 I 2005. pp. 168-176
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