Distal coronary embolization following acute myocardial infarction increases early infarct size and late left ventricular wall thinning in a porcine model

Reuben M. Thomas, Sang Yeob Lim, Beiping Qiang, Azriel B. Osherov, Nilesh R. Ghugre, Hossein Noyan, Xiuling Qi, Rafael Wolff, Michelle Ladouceur-Wodzak, Thomas A. Berk, Jagdish Butany, Mansoor Husain, Graham A. Wright, Bradley H. Strauss

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Distal coronary embolization (DCE) of thrombotic material occurs frequently during percutaneous interventions for acute myocardial infarction and can alter coronary flow grades. The significance of DCE on infarct size and myocardial function remains unsettled. The aims of this study were to evaluate the effects of DCE sufficient to cause no-reflow on infarct size, cardiac function and ventricular remodeling in a porcine acute myocardial infarction model. Methods and results: Female Yorkshire pigs underwent 60 min balloon occlusion of the left anterior descending coronary artery followed by reperfusion and injection of either microthrombi (prepared from autologous porcine blood) sufficient to cause no-reflow (DCE), or saline (control). Animals were sacrificed at 3 h (n = 5), 3 days (n = 20) or 6 weeks (n = 20) post-AMI. Cardiovascular magnetic resonance (CMR), serum troponin-I, and cardiac gelatinase (MMP) and survival kinase (Akt) activities were assessed. At 3d, DCE increased infarct size (CMR: 18.8 % vs. 14.5 %, p = 0.04; serum troponin-I: 13.3 vs. 6.9 ng/uL, p < 0.05) and MMP-2 activity levels (0.81 vs. 0.49, p = 0.002), with reduced activation of Akt (0.06 versus 0.26, p = 0.02). At 6 weeks, there were no differences in infarct size, ventricular volume or ejection fraction between the two groups, although infarct transmurality (70 % vs. 57 %, p< 0.04) and ventricular thinning (percent change in mid anteroseptal wall thickness:-25.6 % vs. 0.7 %, p = 0.03) were significantly increased in the DCE group. Conclusions: DCE increased early infarct size, but without affecting later infarct size, cardiac function or ventricular volumes. The significance of the later remodelling changes (ventricular thinning and transmurality) following DCE, possibly due to changes in MMP-2 activity and Akt activation, merits further study.

Original languageEnglish
Article number106
JournalJournal of Cardiovascular Magnetic Resonance
Volume17
Issue number1
DOIs
Publication statusPublished - 2015 Dec 1

Fingerprint

Matrix Metalloproteinases
Ventricular Remodeling
Troponin I
Swine
Myocardial Infarction
Magnetic Resonance Spectroscopy
Balloon Occlusion
Gelatinases
Ventricular Function
Serum
Reperfusion
Coronary Vessels
Phosphotransferases
Injections

Keywords

  • Angioplasty
  • Cardiovascular magnetic resonance
  • Myocardial infarction
  • No reflow

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine
  • Family Practice

Cite this

Distal coronary embolization following acute myocardial infarction increases early infarct size and late left ventricular wall thinning in a porcine model. / Thomas, Reuben M.; Lim, Sang Yeob; Qiang, Beiping; Osherov, Azriel B.; Ghugre, Nilesh R.; Noyan, Hossein; Qi, Xiuling; Wolff, Rafael; Ladouceur-Wodzak, Michelle; Berk, Thomas A.; Butany, Jagdish; Husain, Mansoor; Wright, Graham A.; Strauss, Bradley H.

In: Journal of Cardiovascular Magnetic Resonance, Vol. 17, No. 1, 106, 01.12.2015.

Research output: Contribution to journalArticle

Thomas, RM, Lim, SY, Qiang, B, Osherov, AB, Ghugre, NR, Noyan, H, Qi, X, Wolff, R, Ladouceur-Wodzak, M, Berk, TA, Butany, J, Husain, M, Wright, GA & Strauss, BH 2015, 'Distal coronary embolization following acute myocardial infarction increases early infarct size and late left ventricular wall thinning in a porcine model', Journal of Cardiovascular Magnetic Resonance, vol. 17, no. 1, 106. https://doi.org/10.1186/s12968-015-0197-y
Thomas, Reuben M. ; Lim, Sang Yeob ; Qiang, Beiping ; Osherov, Azriel B. ; Ghugre, Nilesh R. ; Noyan, Hossein ; Qi, Xiuling ; Wolff, Rafael ; Ladouceur-Wodzak, Michelle ; Berk, Thomas A. ; Butany, Jagdish ; Husain, Mansoor ; Wright, Graham A. ; Strauss, Bradley H. / Distal coronary embolization following acute myocardial infarction increases early infarct size and late left ventricular wall thinning in a porcine model. In: Journal of Cardiovascular Magnetic Resonance. 2015 ; Vol. 17, No. 1.
@article{9450278e661a4200bd84fa271112e16e,
title = "Distal coronary embolization following acute myocardial infarction increases early infarct size and late left ventricular wall thinning in a porcine model",
abstract = "Background: Distal coronary embolization (DCE) of thrombotic material occurs frequently during percutaneous interventions for acute myocardial infarction and can alter coronary flow grades. The significance of DCE on infarct size and myocardial function remains unsettled. The aims of this study were to evaluate the effects of DCE sufficient to cause no-reflow on infarct size, cardiac function and ventricular remodeling in a porcine acute myocardial infarction model. Methods and results: Female Yorkshire pigs underwent 60 min balloon occlusion of the left anterior descending coronary artery followed by reperfusion and injection of either microthrombi (prepared from autologous porcine blood) sufficient to cause no-reflow (DCE), or saline (control). Animals were sacrificed at 3 h (n = 5), 3 days (n = 20) or 6 weeks (n = 20) post-AMI. Cardiovascular magnetic resonance (CMR), serum troponin-I, and cardiac gelatinase (MMP) and survival kinase (Akt) activities were assessed. At 3d, DCE increased infarct size (CMR: 18.8 {\%} vs. 14.5 {\%}, p = 0.04; serum troponin-I: 13.3 vs. 6.9 ng/uL, p < 0.05) and MMP-2 activity levels (0.81 vs. 0.49, p = 0.002), with reduced activation of Akt (0.06 versus 0.26, p = 0.02). At 6 weeks, there were no differences in infarct size, ventricular volume or ejection fraction between the two groups, although infarct transmurality (70 {\%} vs. 57 {\%}, p< 0.04) and ventricular thinning (percent change in mid anteroseptal wall thickness:-25.6 {\%} vs. 0.7 {\%}, p = 0.03) were significantly increased in the DCE group. Conclusions: DCE increased early infarct size, but without affecting later infarct size, cardiac function or ventricular volumes. The significance of the later remodelling changes (ventricular thinning and transmurality) following DCE, possibly due to changes in MMP-2 activity and Akt activation, merits further study.",
keywords = "Angioplasty, Cardiovascular magnetic resonance, Myocardial infarction, No reflow",
author = "Thomas, {Reuben M.} and Lim, {Sang Yeob} and Beiping Qiang and Osherov, {Azriel B.} and Ghugre, {Nilesh R.} and Hossein Noyan and Xiuling Qi and Rafael Wolff and Michelle Ladouceur-Wodzak and Berk, {Thomas A.} and Jagdish Butany and Mansoor Husain and Wright, {Graham A.} and Strauss, {Bradley H.}",
year = "2015",
month = "12",
day = "1",
doi = "10.1186/s12968-015-0197-y",
language = "English",
volume = "17",
journal = "Journal of Cardiovascular Magnetic Resonance",
issn = "1097-6647",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Distal coronary embolization following acute myocardial infarction increases early infarct size and late left ventricular wall thinning in a porcine model

AU - Thomas, Reuben M.

AU - Lim, Sang Yeob

AU - Qiang, Beiping

AU - Osherov, Azriel B.

AU - Ghugre, Nilesh R.

AU - Noyan, Hossein

AU - Qi, Xiuling

AU - Wolff, Rafael

AU - Ladouceur-Wodzak, Michelle

AU - Berk, Thomas A.

AU - Butany, Jagdish

AU - Husain, Mansoor

AU - Wright, Graham A.

AU - Strauss, Bradley H.

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Background: Distal coronary embolization (DCE) of thrombotic material occurs frequently during percutaneous interventions for acute myocardial infarction and can alter coronary flow grades. The significance of DCE on infarct size and myocardial function remains unsettled. The aims of this study were to evaluate the effects of DCE sufficient to cause no-reflow on infarct size, cardiac function and ventricular remodeling in a porcine acute myocardial infarction model. Methods and results: Female Yorkshire pigs underwent 60 min balloon occlusion of the left anterior descending coronary artery followed by reperfusion and injection of either microthrombi (prepared from autologous porcine blood) sufficient to cause no-reflow (DCE), or saline (control). Animals were sacrificed at 3 h (n = 5), 3 days (n = 20) or 6 weeks (n = 20) post-AMI. Cardiovascular magnetic resonance (CMR), serum troponin-I, and cardiac gelatinase (MMP) and survival kinase (Akt) activities were assessed. At 3d, DCE increased infarct size (CMR: 18.8 % vs. 14.5 %, p = 0.04; serum troponin-I: 13.3 vs. 6.9 ng/uL, p < 0.05) and MMP-2 activity levels (0.81 vs. 0.49, p = 0.002), with reduced activation of Akt (0.06 versus 0.26, p = 0.02). At 6 weeks, there were no differences in infarct size, ventricular volume or ejection fraction between the two groups, although infarct transmurality (70 % vs. 57 %, p< 0.04) and ventricular thinning (percent change in mid anteroseptal wall thickness:-25.6 % vs. 0.7 %, p = 0.03) were significantly increased in the DCE group. Conclusions: DCE increased early infarct size, but without affecting later infarct size, cardiac function or ventricular volumes. The significance of the later remodelling changes (ventricular thinning and transmurality) following DCE, possibly due to changes in MMP-2 activity and Akt activation, merits further study.

AB - Background: Distal coronary embolization (DCE) of thrombotic material occurs frequently during percutaneous interventions for acute myocardial infarction and can alter coronary flow grades. The significance of DCE on infarct size and myocardial function remains unsettled. The aims of this study were to evaluate the effects of DCE sufficient to cause no-reflow on infarct size, cardiac function and ventricular remodeling in a porcine acute myocardial infarction model. Methods and results: Female Yorkshire pigs underwent 60 min balloon occlusion of the left anterior descending coronary artery followed by reperfusion and injection of either microthrombi (prepared from autologous porcine blood) sufficient to cause no-reflow (DCE), or saline (control). Animals were sacrificed at 3 h (n = 5), 3 days (n = 20) or 6 weeks (n = 20) post-AMI. Cardiovascular magnetic resonance (CMR), serum troponin-I, and cardiac gelatinase (MMP) and survival kinase (Akt) activities were assessed. At 3d, DCE increased infarct size (CMR: 18.8 % vs. 14.5 %, p = 0.04; serum troponin-I: 13.3 vs. 6.9 ng/uL, p < 0.05) and MMP-2 activity levels (0.81 vs. 0.49, p = 0.002), with reduced activation of Akt (0.06 versus 0.26, p = 0.02). At 6 weeks, there were no differences in infarct size, ventricular volume or ejection fraction between the two groups, although infarct transmurality (70 % vs. 57 %, p< 0.04) and ventricular thinning (percent change in mid anteroseptal wall thickness:-25.6 % vs. 0.7 %, p = 0.03) were significantly increased in the DCE group. Conclusions: DCE increased early infarct size, but without affecting later infarct size, cardiac function or ventricular volumes. The significance of the later remodelling changes (ventricular thinning and transmurality) following DCE, possibly due to changes in MMP-2 activity and Akt activation, merits further study.

KW - Angioplasty

KW - Cardiovascular magnetic resonance

KW - Myocardial infarction

KW - No reflow

UR - http://www.scopus.com/inward/record.url?scp=84952864406&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84952864406&partnerID=8YFLogxK

U2 - 10.1186/s12968-015-0197-y

DO - 10.1186/s12968-015-0197-y

M3 - Article

C2 - 26620277

AN - SCOPUS:84952864406

VL - 17

JO - Journal of Cardiovascular Magnetic Resonance

JF - Journal of Cardiovascular Magnetic Resonance

SN - 1097-6647

IS - 1

M1 - 106

ER -