Distinct Expression Pattern and Post-Transcriptional Regulation of Cell Cycle Genes in the Glandular Epithelia of Avian Ovarian Carcinomas

Jin Young Lee, Wooyoung Jeong, Ji Hye Kim, Jinyoung Kim, Fuller W. Bazer, Jae Yong Han, Gwonhwa Song

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The cell cycle system is controlled in a timely manner by three groups of cyclins, cyclin dependent kinases and cyclin dependent kinase inhibitors. Abnormal alterations of cell cycle regulatory mechanisms are a common feature of many diseases including numerous tumor types such as ovarian cancer. Although a variety of cell cycle regulatory genes are well known in mammalian species including human and mice, they are not well studied in avian species, especially in laying hens which are recognized as an excellent animal model for research relevant to human ovarian carcinogenesis. Therefore, in the present study, we focused on comparative expression and regulation of expression of candidate genes which might be involved in the cell cycle program in surface epithelial ovarian cancer in laying hens. Our current results indicate that expression levels of cell cycle gene transcripts are greater in cancerous as compared to normal ovaries. In particular, cyclin A2 (CCNA2), CCND1, CCND2, CCND3, CCNE2, cyclin dependent kinase 1 (CDK1), CDK3, CDK5, cyclin dependent kinases inhibitor 1A (CDKN1A) and CDKN1B were upregulated predominantly in the glandular epithelia of cancerous ovaries from laying hens. Further, several microRNAs (miRs), specifically miR-1798, miR-1699, miR-223 and miR-1744 were discovered to influence expression of CCND1, CCNE2, CDK1, and CDK3 mRNAs, respectively, via their 3′-UTR which suggests that post-transcriptional regulation of gene expression influences their expression in laying hens. Moreover, miR-1626 influenced CDKN1A expression and miR-222, miR-1787 and miR-1812 regulated CDKN1B expression via their 3′-UTR regions. Collectively, results of the present study demonstrate increased expression of cell cycle-related genes in cancerous ovaries of laying hens and indicate that expression of these genes is post-transcriptionally regulated by specific microRNAs.

Original languageEnglish
Article numbere51592
JournalPLoS One
Volume7
Issue number12
DOIs
Publication statusPublished - 2012 Dec 7
Externally publishedYes

Fingerprint

cdc Genes
cyclin-dependent kinase
Cyclin-Dependent Kinases
carcinoma
cell cycle
epithelium
laying hens
Epithelium
Genes
Cells
CDC2 Protein Kinase
Carcinoma
Ovary
Cell Cycle
Gene Expression Regulation
3' Untranslated Regions
MicroRNAs
Cyclin A2
ovarian neoplasms
genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Distinct Expression Pattern and Post-Transcriptional Regulation of Cell Cycle Genes in the Glandular Epithelia of Avian Ovarian Carcinomas. / Lee, Jin Young; Jeong, Wooyoung; Kim, Ji Hye; Kim, Jinyoung; Bazer, Fuller W.; Han, Jae Yong; Song, Gwonhwa.

In: PLoS One, Vol. 7, No. 12, e51592, 07.12.2012.

Research output: Contribution to journalArticle

Lee, Jin Young ; Jeong, Wooyoung ; Kim, Ji Hye ; Kim, Jinyoung ; Bazer, Fuller W. ; Han, Jae Yong ; Song, Gwonhwa. / Distinct Expression Pattern and Post-Transcriptional Regulation of Cell Cycle Genes in the Glandular Epithelia of Avian Ovarian Carcinomas. In: PLoS One. 2012 ; Vol. 7, No. 12.
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