Distinct responses of a recA::luxCDABE Escherichia coli strain to direct and indirect DNA damaging agents

Jiho Min, Eun Jin Kim, Robert A. Larossa, Man Bock Gu

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

The recombinant Escherichia coli strain DPD2794 containing a recA::luxCDABE fusion is used to detect genotoxicity of various chemicals. Genotoxic agents were previously categorized into two groups, Direct DNA Damaging (DDD) agents and Indirect DNA Damaging (IDD) agents; these two groups have been distinguished with this strain. Minimum detectable concentrations of the DDD agents were about one to five orders of magnitude lower than those of the IDD agents. The response patterns of this strain to DDD agents differed from those to IDD agents in terms of kinetics and the forms of the dose-dependent response. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)61-68
Number of pages8
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume442
Issue number2
DOIs
Publication statusPublished - 1999 Jun 25
Externally publishedYes

Keywords

  • Direct and indirect DNA damage
  • Genotoxicity
  • recA promoter

ASJC Scopus subject areas

  • Genetics
  • Health, Toxicology and Mutagenesis

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