Abstract
Despite numerous studies on late embryogenesis abundant (LEA) proteins, their functions, roles, and localizations during developmental stages in arthropods remain unknown. LEA proteins protect crucial proteins against osmotic stress during the development and growth of various organisms. Thus, in this study, fluorescence in situ hybridization was used to determine the crucial regions protected against osmotic stress as well as the distinctive localization of group 3 (G3) LEA+ cells during brine shrimp development. Several cell types were found to synthesize G3 LEA RNA, including neurons, muscular cells, APH-1+ cells, and renal cells. The G3 LEA+ neuronal cell bodies outside of the mushroom body projected their axonal bundles to the central body, but those inside the mushroom body projected their axonal bundles toward the deutocerebrum without innervating the central body. The cell bodies inside the mushroom body received axons of the G3 LEA+ sensory cells at the medial ventral cup of the nauplius eye. Several glands were found to synthesize G3 LEA RNA during the nauplius stages of brine shrimp, including the sinus, antennal I and II, salt, and three ectodermal glands. This study provides the first demonstration of the formation of G3 LEA+ sinus glands at the emergence stages of brine shrimp. These results suggest that G3 LEA protein is synthesized in several cell types. In particular, specific glands play crucial roles during the emergence and nauplius stages of brine shrimp.
| Original language | English |
|---|---|
| Pages (from-to) | 169-180 |
| Number of pages | 12 |
| Journal | Archives of Insect Biochemistry and Physiology |
| Volume | 89 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2015 Jul 1 |
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Keywords
- Artemia
- Embryonic development
- Epidermal gland
- POU homeobox
- Water stress
ASJC Scopus subject areas
- Physiology
- Biochemistry
- Insect Science
Cite this
Distinctive localization of group 3 late embryogenesis abundant synthesizing cells during brine shrimp development. / Kim, Bo Yong; Song, Hwa Young; Kim, Mi Young; Lee, Bong Hee; Kim, Kyung Joo; Jo, Kyung Jin; Kim, Suhng Wook; Lee, Seung Kwan; Lee, Boo Hyung.
In: Archives of Insect Biochemistry and Physiology, Vol. 89, No. 3, 01.07.2015, p. 169-180.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Distinctive localization of group 3 late embryogenesis abundant synthesizing cells during brine shrimp development
AU - Kim, Bo Yong
AU - Song, Hwa Young
AU - Kim, Mi Young
AU - Lee, Bong Hee
AU - Kim, Kyung Joo
AU - Jo, Kyung Jin
AU - Kim, Suhng Wook
AU - Lee, Seung Kwan
AU - Lee, Boo Hyung
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Despite numerous studies on late embryogenesis abundant (LEA) proteins, their functions, roles, and localizations during developmental stages in arthropods remain unknown. LEA proteins protect crucial proteins against osmotic stress during the development and growth of various organisms. Thus, in this study, fluorescence in situ hybridization was used to determine the crucial regions protected against osmotic stress as well as the distinctive localization of group 3 (G3) LEA+ cells during brine shrimp development. Several cell types were found to synthesize G3 LEA RNA, including neurons, muscular cells, APH-1+ cells, and renal cells. The G3 LEA+ neuronal cell bodies outside of the mushroom body projected their axonal bundles to the central body, but those inside the mushroom body projected their axonal bundles toward the deutocerebrum without innervating the central body. The cell bodies inside the mushroom body received axons of the G3 LEA+ sensory cells at the medial ventral cup of the nauplius eye. Several glands were found to synthesize G3 LEA RNA during the nauplius stages of brine shrimp, including the sinus, antennal I and II, salt, and three ectodermal glands. This study provides the first demonstration of the formation of G3 LEA+ sinus glands at the emergence stages of brine shrimp. These results suggest that G3 LEA protein is synthesized in several cell types. In particular, specific glands play crucial roles during the emergence and nauplius stages of brine shrimp.
AB - Despite numerous studies on late embryogenesis abundant (LEA) proteins, their functions, roles, and localizations during developmental stages in arthropods remain unknown. LEA proteins protect crucial proteins against osmotic stress during the development and growth of various organisms. Thus, in this study, fluorescence in situ hybridization was used to determine the crucial regions protected against osmotic stress as well as the distinctive localization of group 3 (G3) LEA+ cells during brine shrimp development. Several cell types were found to synthesize G3 LEA RNA, including neurons, muscular cells, APH-1+ cells, and renal cells. The G3 LEA+ neuronal cell bodies outside of the mushroom body projected their axonal bundles to the central body, but those inside the mushroom body projected their axonal bundles toward the deutocerebrum without innervating the central body. The cell bodies inside the mushroom body received axons of the G3 LEA+ sensory cells at the medial ventral cup of the nauplius eye. Several glands were found to synthesize G3 LEA RNA during the nauplius stages of brine shrimp, including the sinus, antennal I and II, salt, and three ectodermal glands. This study provides the first demonstration of the formation of G3 LEA+ sinus glands at the emergence stages of brine shrimp. These results suggest that G3 LEA protein is synthesized in several cell types. In particular, specific glands play crucial roles during the emergence and nauplius stages of brine shrimp.
KW - Artemia
KW - Embryonic development
KW - Epidermal gland
KW - POU homeobox
KW - Water stress
UR - http://www.scopus.com/inward/record.url?scp=84931828831&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84931828831&partnerID=8YFLogxK
U2 - 10.1002/arch.21234
DO - 10.1002/arch.21234
M3 - Article
C2 - 25781424
AN - SCOPUS:84931828831
VL - 89
SP - 169
EP - 180
JO - Archives of Insect Biochemistry and Physiology
JF - Archives of Insect Biochemistry and Physiology
SN - 0739-4462
IS - 3
ER -