Distinctive localization of group 3 late embryogenesis abundant synthesizing cells during brine shrimp development

Bo Yong Kim, Hwa Young Song, Mi Young Kim, Bong Hee Lee, Kyung Joo Kim, Kyung Jin Jo, Suhng Wook Kim, Seung Kwan Lee, Boo Hyung Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Despite numerous studies on late embryogenesis abundant (LEA) proteins, their functions, roles, and localizations during developmental stages in arthropods remain unknown. LEA proteins protect crucial proteins against osmotic stress during the development and growth of various organisms. Thus, in this study, fluorescence in situ hybridization was used to determine the crucial regions protected against osmotic stress as well as the distinctive localization of group 3 (G3) LEA+ cells during brine shrimp development. Several cell types were found to synthesize G3 LEA RNA, including neurons, muscular cells, APH-1+ cells, and renal cells. The G3 LEA+ neuronal cell bodies outside of the mushroom body projected their axonal bundles to the central body, but those inside the mushroom body projected their axonal bundles toward the deutocerebrum without innervating the central body. The cell bodies inside the mushroom body received axons of the G3 LEA+ sensory cells at the medial ventral cup of the nauplius eye. Several glands were found to synthesize G3 LEA RNA during the nauplius stages of brine shrimp, including the sinus, antennal I and II, salt, and three ectodermal glands. This study provides the first demonstration of the formation of G3 LEA+ sinus glands at the emergence stages of brine shrimp. These results suggest that G3 LEA protein is synthesized in several cell types. In particular, specific glands play crucial roles during the emergence and nauplius stages of brine shrimp.

Original languageEnglish
Pages (from-to)169-180
Number of pages12
JournalArchives of Insect Biochemistry and Physiology
Volume89
Issue number3
DOIs
Publication statusPublished - 2015 Jul 1

Fingerprint

Artemia
Embryonic Development
embryogenesis
Mushroom Bodies
mushroom bodies
Proteins
Cells
cells
RNA
nauplii
Osmotic Pressure
Neurons
sinuses
osmotic stress
Demonstrations
Salts
Fluorescence
proteins
neurons
brine

Keywords

  • Artemia
  • Embryonic development
  • Epidermal gland
  • POU homeobox
  • Water stress

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Insect Science

Cite this

Distinctive localization of group 3 late embryogenesis abundant synthesizing cells during brine shrimp development. / Kim, Bo Yong; Song, Hwa Young; Kim, Mi Young; Lee, Bong Hee; Kim, Kyung Joo; Jo, Kyung Jin; Kim, Suhng Wook; Lee, Seung Kwan; Lee, Boo Hyung.

In: Archives of Insect Biochemistry and Physiology, Vol. 89, No. 3, 01.07.2015, p. 169-180.

Research output: Contribution to journalArticle

Kim, Bo Yong ; Song, Hwa Young ; Kim, Mi Young ; Lee, Bong Hee ; Kim, Kyung Joo ; Jo, Kyung Jin ; Kim, Suhng Wook ; Lee, Seung Kwan ; Lee, Boo Hyung. / Distinctive localization of group 3 late embryogenesis abundant synthesizing cells during brine shrimp development. In: Archives of Insect Biochemistry and Physiology. 2015 ; Vol. 89, No. 3. pp. 169-180.
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