Distinguishing Ophiostoma ips and Ophiostoma montium, two bark beetle-associated sapstain fungi

Jae Jin Kim; Seong Hwan Kim; Sangwon Lee; Colette Breuil

doi:10.1016/S0378-1097(03)00304-5

Distinguishing Ophiostoma ips and Ophiostoma montium, two bark beetle-associated sapstain fungi

Jae Jin Kim, Seong Hwan Kim, Sangwon Lee, Colette Breuil

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

Two synonymous sapstain species, Ophiostoma montium and Ophiostoma ips, which are vectored by Dendroctonus ponderosae and various bark beetles, respectively, were differentiated into separate species using growth and molecular characteristics. Analysis of 32 isolates of the two species from different countries showed that O. ips was able to grow at 35°C while O. montium was not. This growth-based differentiation was strongly supported by sequence data for the internal transcribed spacer (ITS), 5.8S and partial 28S rDNA, and the β-tubulin genes. The β-tubulin gene sequence data indicate that the two species can easily be differentiated with a single polymerase chain reaction (PCR) assay.

Original language	English
Pages (from-to)	187-192
Number of pages	6
Journal	FEMS microbiology letters
Volume	222
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(03)00304-5
Publication status	Published - 2003 May 28
Externally published	Yes

Keywords

Bark beetle
Ophiostoma ips
Ophiostoma montium
Sapstain
rDNA
β-tubulin gene

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/S0378-1097(03)00304-5

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title = "Distinguishing Ophiostoma ips and Ophiostoma montium, two bark beetle-associated sapstain fungi",

abstract = "Two synonymous sapstain species, Ophiostoma montium and Ophiostoma ips, which are vectored by Dendroctonus ponderosae and various bark beetles, respectively, were differentiated into separate species using growth and molecular characteristics. Analysis of 32 isolates of the two species from different countries showed that O. ips was able to grow at 35°C while O. montium was not. This growth-based differentiation was strongly supported by sequence data for the internal transcribed spacer (ITS), 5.8S and partial 28S rDNA, and the β-tubulin genes. The β-tubulin gene sequence data indicate that the two species can easily be differentiated with a single polymerase chain reaction (PCR) assay.",

keywords = "Bark beetle, Ophiostoma ips, Ophiostoma montium, Sapstain, rDNA, β-tubulin gene",

author = "Kim, {Jae Jin} and Kim, {Seong Hwan} and Sangwon Lee and Colette Breuil",

note = "Funding Information: This work was supported by the Natural Sciences and Engineering Research Council of Canada. J.-J.K. was supported by a Postdoctoral Fellowship Program from the Korea Science and Engineering Foundation. We thank Drs. H. Masuya, H. Solheim, and A. Uzunovic for providing O. ips and O. montium cultures. ",

year = "2003",

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doi = "10.1016/S0378-1097(03)00304-5",

language = "English",

volume = "222",

pages = "187--192",

journal = "FEMS Microbiology Letters",

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T1 - Distinguishing Ophiostoma ips and Ophiostoma montium, two bark beetle-associated sapstain fungi

AU - Kim, Jae Jin

AU - Kim, Seong Hwan

AU - Lee, Sangwon

AU - Breuil, Colette

N1 - Funding Information: This work was supported by the Natural Sciences and Engineering Research Council of Canada. J.-J.K. was supported by a Postdoctoral Fellowship Program from the Korea Science and Engineering Foundation. We thank Drs. H. Masuya, H. Solheim, and A. Uzunovic for providing O. ips and O. montium cultures.

PY - 2003/5/28

Y1 - 2003/5/28

N2 - Two synonymous sapstain species, Ophiostoma montium and Ophiostoma ips, which are vectored by Dendroctonus ponderosae and various bark beetles, respectively, were differentiated into separate species using growth and molecular characteristics. Analysis of 32 isolates of the two species from different countries showed that O. ips was able to grow at 35°C while O. montium was not. This growth-based differentiation was strongly supported by sequence data for the internal transcribed spacer (ITS), 5.8S and partial 28S rDNA, and the β-tubulin genes. The β-tubulin gene sequence data indicate that the two species can easily be differentiated with a single polymerase chain reaction (PCR) assay.

AB - Two synonymous sapstain species, Ophiostoma montium and Ophiostoma ips, which are vectored by Dendroctonus ponderosae and various bark beetles, respectively, were differentiated into separate species using growth and molecular characteristics. Analysis of 32 isolates of the two species from different countries showed that O. ips was able to grow at 35°C while O. montium was not. This growth-based differentiation was strongly supported by sequence data for the internal transcribed spacer (ITS), 5.8S and partial 28S rDNA, and the β-tubulin genes. The β-tubulin gene sequence data indicate that the two species can easily be differentiated with a single polymerase chain reaction (PCR) assay.

KW - Bark beetle

KW - Ophiostoma ips

KW - Ophiostoma montium

KW - Sapstain

KW - rDNA

KW - β-tubulin gene

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DO - 10.1016/S0378-1097(03)00304-5

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AN - SCOPUS:0037721535

SN - 0378-1097

VL - 222

SP - 187

EP - 192

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

Distinguishing Ophiostoma ips and Ophiostoma montium, two bark beetle-associated sapstain fungi

Abstract

Keywords

ASJC Scopus subject areas

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