Distinguishing Ophiostoma ips and Ophiostoma montium, two bark beetle-associated sapstain fungi

Jae Jin Kim, Seong Hwan Kim, Sangwon Lee, Colette Breuil

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Two synonymous sapstain species, Ophiostoma montium and Ophiostoma ips, which are vectored by Dendroctonus ponderosae and various bark beetles, respectively, were differentiated into separate species using growth and molecular characteristics. Analysis of 32 isolates of the two species from different countries showed that O. ips was able to grow at 35°C while O. montium was not. This growth-based differentiation was strongly supported by sequence data for the internal transcribed spacer (ITS), 5.8S and partial 28S rDNA, and the β-tubulin genes. The β-tubulin gene sequence data indicate that the two species can easily be differentiated with a single polymerase chain reaction (PCR) assay.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalFEMS microbiology letters
Volume222
Issue number2
DOIs
Publication statusPublished - 2003 May 28

Keywords

  • Bark beetle
  • Ophiostoma ips
  • Ophiostoma montium
  • Sapstain
  • rDNA
  • β-tubulin gene

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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